Quantitative character gene site locating method based genomic exon chip

A technology of gene loci and quantitative traits, applied in the fields of biotechnology and genetic engineering, can solve the problems of sequence information (probe sequence application value cannot be fully utilized, etc.), and achieve the effect of wide application value

Inactive Publication Date: 2003-10-15
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the characteristics of these data, the application value of its sequence information (probe sequence) cannot be fully utilized

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Embodiment 1, the present invention is realized through the following steps

[0022] Step 1: Construct genetic populations Construct plant genetic populations (such as DH populations, F2 populations, near-isogenic lines, recombinant inbred lines, etc.) or animal families;

[0023] Step 2: Build a molecular marker map Collect and determine all known and annotated gene sequence data and position data on chromosomes of related species through public molecular databases (GenBank, etc.), and establish a molecular marker map of the so-called population;

[0024] Step 3: Make an exon sequence chip Make a genome-wide or specific chromosome exon sequence chip

[0025] Step 4: Collect chip data and conduct DNA sampling and chip hybridization experiments on individuals in the research group within a certain period of time to obtain chip signal data;

[0026] Step 5: Microarray data processing Analyze the expression difference and significance of the difference on the microarray d...

Embodiment 2

[0031] Example 2: Mapping and cloning of genes controlling plant height in rice

[0032] Step 1: Construct a Genetic Population

[0033] A rice DH population with 96 individuals in total. The purpose of establishing this group is to map the QTL of rice plant height traits

[0034] Step 2: Construction of Molecular Marker Atlas

[0035] Collect and determine all known and annotated gene sequence data (currently known 50,000-60,000) of rice through public molecular databases (GenBank, etc.) and the position data of gene exons on the chromosome to establish molecular markers of rice Atlas.

[0036] In this example, there are 21 exons (Marker#), and their physical positions in the three chromosomes (ch1 / ch2 / ch3) (the total number of bases from the first base at the 5' end of the exon to the starting base of the chromosome, The data in this example have been divided by 100000) see the table below. Due to multiple copies and other reasons, some exons are distributed on multiple...

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PUM

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Abstract

The new quantitative character gene site locating method based on genome exon chip is to utilize exon sequence as one kind of molecular mark and utilize the detected data of human, rice and other allgenome or exon sequence chip of partial chromosome as colony locating molecular mark data for gene location via statistic location method. The present invention can obtain located functional gene sequence directly, and is suitable for all the quantitative character and complicated character of life. The research colonies include plant DH colony, F2 colony, near isogenic line, near isogenic introduction line, recombinant idiogamy line, animal family, etc.

Description

technical field [0001] The invention belongs to the field of biotechnology and genetic engineering, and relates to a new method for positioning and cloning quantitative / complex trait gene loci (QTL) based on gene exon chip data of genome or partial chromosomes, which is applicable to all biological quantitative traits or Complex trait (disease) gene mapping. technical background [0002] The use of DNA molecular markers and specific population data to map quantitative trait genes (Quantitative trait) or complex trait (Complex trait) loci (QTLs) is an important development in genetics in recent years, and a number of molecular marker-based mapping methods have been proposed. (Lander and Botstein, 1989, Genetics, 121: 185-199; Zeng, 1994, Genetics, 136: 1457-1468; Lander and Kruglyak, 1995, Nat. Genet. 11: 241-247; Zhu Jun, 1998, China Agricultural Science and Technology Press, 11-20; Wang et al., 1999, Theor.Appl.Genet., 99:1255-1264; Almasy and Brangero, 1998, Am.J.Hum.Gene...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68
Inventor 樊龙江
Owner ZHEJIANG UNIV
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