Quantitative character gene site locating method based genomic exon chip
A technology of gene loci and quantitative traits, applied in the fields of biotechnology and genetic engineering, can solve the problems of sequence information (probe sequence application value cannot be fully utilized, etc.), and achieve the effect of wide application value
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Embodiment 1
[0021] Embodiment 1, the present invention is realized through the following steps
[0022] Step 1: Construct genetic populations Construct plant genetic populations (such as DH populations, F2 populations, near-isogenic lines, recombinant inbred lines, etc.) or animal families;
[0023] Step 2: Build a molecular marker map Collect and determine all known and annotated gene sequence data and position data on chromosomes of related species through public molecular databases (GenBank, etc.), and establish a molecular marker map of the so-called population;
[0024] Step 3: Make an exon sequence chip Make a genome-wide or specific chromosome exon sequence chip
[0025] Step 4: Collect chip data and conduct DNA sampling and chip hybridization experiments on individuals in the research group within a certain period of time to obtain chip signal data;
[0026] Step 5: Microarray data processing Analyze the expression difference and significance of the difference on the microarray d...
Embodiment 2
[0031] Example 2: Mapping and cloning of genes controlling plant height in rice
[0032] Step 1: Construct a Genetic Population
[0033] A rice DH population with 96 individuals in total. The purpose of establishing this group is to map the QTL of rice plant height traits
[0034] Step 2: Construction of Molecular Marker Atlas
[0035] Collect and determine all known and annotated gene sequence data (currently known 50,000-60,000) of rice through public molecular databases (GenBank, etc.) and the position data of gene exons on the chromosome to establish molecular markers of rice Atlas.
[0036] In this example, there are 21 exons (Marker#), and their physical positions in the three chromosomes (ch1 / ch2 / ch3) (the total number of bases from the first base at the 5' end of the exon to the starting base of the chromosome, The data in this example have been divided by 100000) see the table below. Due to multiple copies and other reasons, some exons are distributed on multiple...
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