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Molecular markers in close linkage with large grain gene GS2 of rice and application thereof

A molecular marker and rice technology, applied in the field of agricultural biology, can solve the problem of unreached genetic distance

Active Publication Date: 2013-11-27
HUNAN HYBRID RICE RES CENT
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  • Abstract
  • Description
  • Claims
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Problems solved by technology

However, in this study, the qGS2 gene was located in a relatively large interval, and the genetic distance was far from meeting the requirements of practical molecular markers

Method used

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  • Molecular markers in close linkage with large grain gene GS2 of rice and application thereof
  • Molecular markers in close linkage with large grain gene GS2 of rice and application thereof
  • Molecular markers in close linkage with large grain gene GS2 of rice and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0102] Example 1 Acquisition and Application of Molecular Markers Tightly Linked to Rice Large Grain Gene GS2

[0103] (1) Positioning population and phenotypic identification

[0104] During the 2011-2012 Hainan Southern breeding period, in the breeding materials [F 6 , R1126 (the length of rice grains is close to 10mm) / Chuandali (the length of rice grains is about 13mm, provided by the Rice and Sorghum Research Institute of Sichuan Academy of Agricultural Sciences), from F 2 It was found that the agronomic traits of some lines were basically stable except for the segregation of grain shape. Genetic analysis showed that the grain shape showed the characteristics of single-gene segregation. Through the investigation of two seasons in Hainan and one season in Changsha, it was found that the material The segregation of grain shape conformed to the characteristics of single gene segregation, and the difference of grain shape was obvious, and the large grain showed incomplete dom...

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Abstract

The invention provides molecular markers in close linkage with a large grain gene GS2 of rice and an application of the molecular markers. Through a recombinant inbred line (RIL) advanced grain shape segregation population, the grain shape major gene GS2 is finely located on a second chromosome and within a range of 33.2kb from GS2-6 to GS2-8, so as to obtain a series of molecular markers in close linkage with GS2. The molecular markers include a common data base marker RM3212 and 22 self-developed InDel markers (from GS2-1 to GS2-22). The molecular markers can be used for effectively detecting the GS2 genetic locus and further improving the breeding efficiency of large grain varieties (or parents).

Description

technical field [0001] The invention belongs to the field of agricultural biotechnology, and in particular relates to a molecular marker closely linked with rice large-grain gene GS2 and its application. Background technique [0002] More than half of the world's population uses rice as a staple food, and rice is one of the most important food crops. Rice grain shape not only affects 1000-grain weight and then indirectly affects yield, but also has an important impact on rice quality. Many consumers at home and abroad have a preference for indica rice varieties with slender grains. In the "National Standard of the People's Republic of China: High-quality Rice GB / T17891-1999", it is clearly stipulated that the rice aspect ratio of high-quality rice should not be lower than 2.8. Therefore, cultivating long-grained rice varieties is of great significance for improving rice yield and rice quality. [0003] The genetic basis of grain shape in rice is complex and is a typical qu...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/11C12Q1/68
Inventor 邓华凤张武汉孙平勇何强舒服邢俊杰王杰
Owner HUNAN HYBRID RICE RES CENT
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