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Protein, gene and function fragment involved in palmitic acid synthesis and application thereof

A palmitic acid and gene technology, applied in the field of proteins involved in palmitic acid synthesis, can solve the problems of slow development of high-oil corn, long innovation time of germplasm resources, slow progress, etc.

Inactive Publication Date: 2011-01-19
CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The market prospect of high-oil corn is very broad, but the development of high-oil corn is very slow due to various reasons such as narrow germplasm resources, long innovation time of germplasm resources, slow progress, and high cost.

Method used

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  • Protein, gene and function fragment involved in palmitic acid synthesis and application thereof
  • Protein, gene and function fragment involved in palmitic acid synthesis and application thereof
  • Protein, gene and function fragment involved in palmitic acid synthesis and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] Embodiment 1, the discovery of gene and functional fragment

[0042] 1. QTL-Pa19 fine mapping and cloning

[0043] 1. Construction and evaluation of QTL-Pa19 near isogenic line

[0044] A near-isogenic line for the target gene of QTL-Pa19 was constructed using a high-generation backcross strategy, such as figure 1 As shown, RIL129 from the recombinant inbred line population obtained from the crossing of B73 and By804 was selected as the donor parent. About 44.5% of the genetic background of this line came from B73, which carried the By804 fragment in the target genome region and had relatively High absolute palmitic acid content and high proportion of palmitic acid; B73, as a recurrent parent, has significant differences in target traits from By804 and RIL129. Each generation uses molecular markers to control the genetic background, and uses association analysis and linkage population progeny verification to evaluate the target traits of the population with favorable re...

Embodiment 2

[0086] Example 2, Functional verification of ZmFatB (prokaryotic expression)

[0087] 1. Basic principles

[0088] The in vivo expression results confirmed that there was no difference in the expression of ZmFatB alleles, so the indel at the 3' end 11bp is very likely to lead to differences in protein activity, resulting in phenotypic variation. There are two alternative outcomes for plastid fatty acid synthesis in plants. Part of the formed acyl carrier protein is directly converted into glycerol lipids in plastids, and palmitic acid in the form of C16:0-ACP can be directly converted into plastids Prokaryotic lipid metabolism is catalyzed by acyltransferase, while the other part is hydrolyzed into free fatty acids and acyl carrier protein (ACP) by acyl-ACP thioesterases (FAT). Free fatty acids enter the cytoplasm and further participate in the "eukaryotic pathway" of lipid metabolism - the Kennedy pathway (Kennedy pathway). Therefore, the FATB gene acts on the plastid and p...

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Abstract

The invention discloses a protein, a gene and a function fragment involved in palmitic acid synthesis and application thereof. The invention provides a protein composed of amino acid sequences shown by a sequence 6, a sequence 4 or a sequence 2 in a sequence table, a DNA (Deoxyribonucleic Acid) molecule shown by a sequence 5, a sequence 3 or a sequence 1 in the sequence table, and a DNA fragment shown by a sequence 7. In the invention, a recombinant inbred strain colony is constructed by utilizing a common material B73 and a high oil system By 804, a major QTL-Pa 19 for controlling corn oil palmitic acid is positioned on a ninth chromosome 9.02 Bin, and the LOD (Level of Detail) of the major QTL-Pa can reach 27, and can be used for interpreting 42% of genetic variation. By means of the method of based cloning, the invention accurately positions the major QTL-Pa for controlling the palmitic acid content of corn kernels and the proportion of kernel saturated / unsaturated fatty acid in Beijing high oil, clones the function gene of QTL-Pa 19, probes the functional sites thereof, develops the functional markers, and has great value for improving the quality of corn kernels.

Description

technical field [0001] The invention relates to proteins, genes and functional fragments involved in palmitic acid synthesis and their application. Background technique [0002] Corn is one of the most important energy sources for human beings, and its grain fatty acid content and component ratio directly affect human health. The World Health Organization proposes that corn oil is the most health-care healthy vegetable oil. It is rich in vitamin E and unsaturated fatty acids necessary for the human body. Its oleic acid content is 24.4%, while linoleic acid can exceed 58%. Long-term consumption can prevent obesity. Atherosclerosis and cholesterol accumulation, lower blood pressure. The market prospect of high-oil corn is very broad, but the development of high-oil corn is very slow due to various reasons such as limited germplasm resources, long innovation time of germplasm resources, slow progress, and high cost. With the development of molecular biology and its applicatio...

Claims

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Application Information

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IPC IPC(8): C07K14/415C12N15/29C12N15/63C12N5/10C12N15/11C12N1/21C12R1/19
Inventor 李建生严建兵李林李慧郑德波张义荣郑艳萍
Owner CHINA AGRI UNIV
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