Molecular marker closely linked with bacterial wilt resistance of cultivated peanuts and application of molecular marker

A peanut bacterial wilt and molecular marker technology, applied in the field of molecular biology, can solve the problem of inability to obtain molecular markers for bacterial wilt resistance molecular markers and the like

Active Publication Date: 2021-10-29
HENAN ACAD OF AGRI SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Although the QTL mapping research of bacterial wilt resistance has been carried out in peanuts in recent years, and some resistance markers have been obtained, which provide a theoretic

Method used

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  • Molecular marker closely linked with bacterial wilt resistance of cultivated peanuts and application of molecular marker
  • Molecular marker closely linked with bacterial wilt resistance of cultivated peanuts and application of molecular marker
  • Molecular marker closely linked with bacterial wilt resistance of cultivated peanuts and application of molecular marker

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Experimental program
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Embodiment 1

[0027] Example 1 Acquisition of main QTL for resistance to bacterial wilt of cultivar Peanut Yuanza 9102

[0028] (1) The recombinant inbred line populations of Yuanza 9102 and wt09-0023 (highly susceptible to bacterial wilt) were sequenced using simplified genome sequencing technology. The sequencing depth of the two parents was greater than 20×, and the sequencing depth of the progeny material was 5 ×. The genotyping data of the parents and offspring were obtained based on the genome information of cultivated peanut species (Arachis hypogaea.cv.Tifrunner V1.0). A total of more than 90,000 polymorphic SNPs with homozygous genotypes were obtained from the two parents.

[0029] According to the whole genome resequencing results of Yuanza 9102 and wt09-0023, KASP primers for 17 SNP sites were designed, and the genotyping data of the parents and offspring of the population were obtained through the genotyping platform. Filter out the SNP markers with a deletion rate of more tha...

Embodiment 2

[0032] Example 2 Identification of Molecular Markers Tightly Linked to the Major QTL for Bacterial Wilt Resistance in Yuanza 9102 Derivative Lines and Natural Populations

[0033](1) According to the genome sequence information of the SNP positions on the left and right flanks of the main QTL (qBWR_A12), 8 sets of KASP primers were developed (see Table 1), and these SNP sites were verified by the SNPLine genotyping platform (LGC) in Yuanza 9102 and wt09-0023 in recombinant inbred lines whose genotyping results were consistent with the sequencing results.

[0034] Table 1 KASP primer sequence information

[0035]

[0036] (2) Select 47 Yuanza 9102 derived line materials (see Table 2) and 317 natural population materials (see Table 3) to carry out resistance phenotype identification in the bacterial wilt disease nursery of Hezhou Peanut Comprehensive Experimental Station, Guangxi Province . The 47 materials derived from Yuanza 9102 are intermediate breeding materials from d...

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Abstract

The invention relates to a molecular marker closely linked with bacterial wilt resistance of cultivated peanuts and application of the molecular marker. A recombinant inbred line group taking far-hybrid 9102 and wt09-0023 as parents is utilized to construct a high-density linkage map, and a resistance major QTL (qBWR-A12) which can be repeated in a plurality of growth periods and for many years is positioned. KASP primers are developed according to SNP information of two wings of the major QTL, an SNP molecular marker capable of being applied to far-hybrid 9102 blood-related materials and non-blood-related materials is obtained, the SNP marker is located at the 4097252bp position of the 12th chromosome, and linkage between the marker developed based on the QTL and bacterial wilt resistance is tighter. According to the molecular marker, the step of bacterial wilt inoculation identification or disease nursery identification is omitted, the bacterial wilt resistance information of the material can be rapidly and accurately obtained only by detecting the SNP genotype, and the molecular marker can be applied to molecular breeding of peanut bacterial wilt resistance.

Description

technical field [0001] The invention relates to a molecular marker closely linked with the resistance of cultivated peanut bacterial wilt and its application, which belongs to the field of molecular biology. Background technique [0002] Ralstonia solanacearum is a bacterial disease caused by Ralstonia solanacearum, which is distributed all over the world. Bacterial wilt is a soil-borne disease. The pathogen infects plants mainly through wounds in the roots of plants, and spreads rapidly in the plant vascular system, causing the aboveground parts to wilt due to lack of water. Rattlesia can infect more than 450 species of plants, including Solanaceae, Cruciferae, Gramineae, etc. More than 90 species of plants are infected in China, among which peanuts, tomatoes, tobacco and other crops are affected in more than ten provinces. The arable land affected by bacterial wilt has reached 800,000 hectares, accounting for nearly 16% of the national planting area. In some diseased are...

Claims

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Application Information

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IPC IPC(8): C12N15/11C12Q1/6895
CPCC12Q1/6895C12Q2600/13C12Q2600/156
Inventor 张新友齐飞艳孙子淇刘华郑峥秦利黄冰艳田梦迪石磊苗利娟董文召
Owner HENAN ACAD OF AGRI SCI
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