SNP molecular marker related to skin color character of black-bone chicken and application

Abstract

The invention provides application of an SNP molecular marker in detecting the skin color character of a black-bone chicken. The nucleotide sequence of the molecular marker is shown as SEQ ID No.1, and the base mutation type at the 171st bp is A / G. The invention further provides a primer sequence for detecting an SNP locus and a PCR-RFLP method. The genotype of the locus can be distinguished based on the difference of an electrophoretic band after DraI enzyme digestion, and then the homozygosis and heterozygosis of a sample Fm gene can be judged. If the 342 bp band appears, the sample Fm gene is a GG homozygosis-based gene; if the two bands of 171 bp and 342 bp appear, the sample Fm gene is an AG heterozygosis-based gene; if the 171 bp band appears, the sample Fm gene is an AA recessive-homozygosis-based gene. By means of the SNP molecular marker and the detection method, the individual genotype of the black-bone chicken can be judged in the chickling stage, early selection and early elimination of the skin color character can be achieved, and thus significant practical value can be achieved on improving selection efficiency and saving breeding cost.

Description

technical field [0001] The invention relates to a SNP marker used for detecting homozygous and heterozygous Fm genes related to skin color traits of black-bone chickens, and belongs to the field of agricultural biotechnology. Background technique [0002] Black-bone chicken has a very long history of breeding in my country. It usually has the characteristics of black skin, black bones, and black meat. It is a traditional and rare chicken species for both medicine and food in my country. It is mild, non-toxic, tonic for deficiency and fatigue, cures thirst, benefits puerpera, treats women's collapse and leukorrhea, and all diseases of deficiency and loss", so it is deeply favored by consumers. Silkie breeds mainly include Taihe Silky Feather Silky, Dongxiang Green-shell Layer, Jiangshan Silky, etc., all of which are commonly used as excellent materials in silky breeding. However, with the introduction of foreign chicken breeds and mixed blood, There are cases of incomplete bl...

AI Technical Summary

Problems solved by technology

Therefore, it is impossible to judge whether the genotype is homozygous based on the phenotype of the individual. Although it can be verified by test crossing with white-skinned chicken breeds, the test cross cycle is long and expensive, and it is difficult to implement it in the whole group

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