Rapid breeding method of imidazolinone herbicide resistant rice germplasm

An imidazolinone- and herbicide-resistant technology is applied in the field of rapid breeding of rice germplasm resistant to imidazolinone-type herbicides. It can solve the problems of manual removal, time-consuming and labor-intensive offspring materials, and few offspring groups, so as to improve efficiency and Accuracy, save manpower time, improve the effect of selection efficiency

Pending Publication Date: 2020-12-22
COASTAL AGRI RES INST HEBEI ACAD OF AGRI & FORESTRY SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, it has the following defects: (1) There are few offspring groups covered by detection, and it is easy to lose offspring resources
(2) The late selection is time-consuming and labor-intensive
(3) It is impossible to test the true and false hybrids after hybridization
(4) Inaccurate detection occurs
The main reasons are: (1) It takes time and effort to detect a large number of progeny materials by using molecular markers, and if the detection is not comprehensive, it is easy to lose valuable progeny resources
(2) The experiment can only be carried out after the F1 generation grows out. After the identification, it is necessary to manually remove the non-resistant individual plants in the field, and then select the herbicide-resistant golden rice germplasm
(3) The detection of the band type can only reveal the presence or absence of the resistance gene, and cannot detect true and false hybrids in the F1 generation
(4) Because of the use of linkage markers, there will be cases where genes break the linkage, so there will be inaccurate detection

Method used

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  • Rapid breeding method of imidazolinone herbicide resistant rice germplasm

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Experimental program
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Effect test

Embodiment 1

[0023]The invention provides a rapid breeding method for rice germplasm resistant to imidazolinone herbicides. The offspring is separated by crossing the herbicide-resistant material and the non-resistant material by using a specific concentration of herbicide to soak seeds and spray during the seedling period, which is fast and accurate Excluding non-resistant offspring materials, the surviving plants are herbicide-resistant and can grow normally, which is convenient for selection. The invention includes the use of specific concentration and herbicide to soak seeds, the specific concentration of herbicide is sprayed in the seedling stage, the concentration of soaking seeds is 0.03%, the time is 72 hours, and the spray concentration is 0.1%. Specifically include the following:

[0024]a. Use the selected imidazolinone-resistant herbicide varieties Jinjing 818 or Jindao 372 as the resistant gene donor parent, and the other parent is selected according to the breeding requirements, and t...

Embodiment 2

[0031]Use herbicides of different concentrations to soak the seeds to kill the non-herbicide resistant materials, and set the herbicide concentration: 0, 0.0005%, 0.001%, 0.003%, 0.005%, 0.007%, 0.01%, 0.03%, 0.05%, 0.07%, 0.1%, 0.3%, 0.5%, 0.7%. The results are shown in Tables 1 and 2. 0-13 means 0-0.7% from left to right.

[0032]Table 1 Survival of materials under 14 concentration gradients of 0-0.7% (sow 50 seeds)

[0033] concentration 012345678910111213 Kenyu 60 50504037282251000000 Tsuda 372 5050505050505050403530211010

[0034]Table 2 Survival amount of material for 3 seed soaking time under 4 concentration gradients of 0.01%-0.07% (sowing 50 seeds)

[0035]

[0036]The experimental results show that the use of 0.03% herbicide, soaking seeds for 72 hours, can effectively kill non-herbicide-resistant materials with the best effect.

Embodiment 3

[0038]Planting seedling trays to simulate rice seedlings by spraying herbicides to remove non-resistant materials. Set herbicide concentrations: 0, 0.04%, 0.06%, 0.08%, 0.1%, 0.12%, and spray weeds when the rice grows to two leaves and one heart. Spray twice in a row with an interval of 3 days. The results are shown in Table 3.

[0039] Table 3 Seedling survival rate after spraying herbicide in seedling tray

[0040] Variety / concentration00.04％0.06％0.08％0.10％0.12％ Kenyu 60 100％78％43％5％0％0％ Tsuda 372 100％100％100％100％100％95％

[0041] The experimental results show that the herbicide with a concentration of 0.1% can be used during the seedling stage to kill materials that are not resistant to herbicides.

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Abstract

The invention discloses a rapid breeding method of imidazolinone herbicide resistant rice germplasm. According to the method, herbicide with a specific concentration is used for performing seed soaking and spraying in the seedling stage on hybridization and separation progeny of herbicide-resistant materials and non-resistant materials so as to rapidly and accurately remove non-resistant progeny materials, survived plants are herbicide-resistant and can normally grow, and seed selection is facilitated. The method comprises the steps that the seeds are soaked with the herbicide with the specific concentration, the herbicide with the specific concentration is sprayed in the seedling stage, the seed soaking concentration is 0.03%, and the seed soaking time is 72 h. The spraying concentrationis 0.1%, and the spraying amount is 30ml / m<2>. The method can improve the efficiency and accuracy of the herbicide-resistant germplasm breeding process.

Description

Technical field[0001]The invention belongs to the technical field of resistant rice breeding, and specifically relates to a rapid breeding method for imidazolinone herbicide-resistant rice germplasm.Background technique[0002]Rice is the main food crop in my country and the world, and the main source of heat for nearly half of the world's population. A large number of weeds have always occurred in the rice fields of all rice-growing areas in my country. According to statistics, there are more than 200 species of weeds in rice fields in the country, of which about 40 species are common, serious and the most common weeds (10-20 species distributed in various rice areas). In my country, the control of weeds and weedy rice in paddy fields mainly involves manual weeding, weeding of farm tools and chemical weeding. Manual weeding and weeding of farm tools are labor-intensive and low-efficiency, and cannot be controlled in a large area in time. The current chemical methods cannot remove wee...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H1/02A01H1/04
CPCA01H1/02A01H1/04
Inventor 邹拓耿雷跃张薇杜琪左永梅张启星孟令启李建广
Owner COASTAL AGRI RES INST HEBEI ACAD OF AGRI & FORESTRY SCI
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