Method for culturing chlamys farreri trochophore cell line

A technology for Chlamys farreri and trochophore larvae, applied to animal cells, etc., can solve problems such as inability to subculture shellfish cells

Inactive Publication Date: 2013-03-13
OCEAN UNIV OF CHINA
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Problems solved by technology

[0014] The invention provides a method for cultivating the trochophore cell line of Chlamys farreri, aiming to solve the problem that the subculture of shellfish cells cannot be realized in the prior art

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Embodiment Construction

[0029] In order to make the purpose, technical solution and advantages of the present invention more clear, the present invention will be further described in detail below in conjunction with the accompanying drawings and embodiments. It should be understood that the specific embodiments described here are only used to explain the present invention, not to limit the invention.

[0030] figure 1 It shows the implementation flow of the method for cultivating the Chlamys farreri troponin larvae cell line provided by the embodiment of the present invention.

[0031] This culture method comprises the following steps:

[0032] Step S101, washing the sterile seawater filtered by a positive pressure filter with running water on a 500-mesh sieve silk for more than half an hour to remove microorganisms on the surface;

[0033] Step S102, using calcium-free seawater isotonic buffer specially used for Chlamys farreri troponiform larvae cells to soak for more than 30 minutes, and mechani...

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Abstract

The invention discloses a method for culturing a chlamys farreri trochophore cell line. A small amount of nutrient factors for keeping cell metabolism are added in a special cell culture medium for chlamys farreri trochophore, so that the good growth state of chlamys farreri trochophore larva in vitro culture cells is realized, and the stable subcultring of the cells can be realized along with cell division. The method is simple to operate and low in cost; the complete dissociation of the cells is realized by use of the combination of soaking in a special calcium-free sea-water iso-osmia buffer solution for the chlamys farreri trochophore and a mechanical method, without any digestion by enzymes; the method is little in damage of the chlamys farreri trochophore larva in vitro culture cells, low in cost, high in efficiency and repeatable; a stable marine invertebrate cell in vitro culture method is established and can realize the in vitro multiplication and the subcultring of the cell, the chlamys farreri trochophore cell line is the first continuous cell line of marine shells, and the method has an important guiding significance for cell line establishment of other shells except the chlamys farreri.

Description

technical field [0001] The invention belongs to the technical field of cell culture, and in particular relates to a method for cultivating a Chlamys farreri troponin larvae cell line. Background technique [0002] So far, no one at home and abroad has established an in vitro culture system for marine bivalve cells, extending the scope to molluscs. At present, only one cell line has been successfully established, which comes from a freshwater snail. [0003] Cell materials: Cells cultured in vitro are mostly obtained from animal embryos, adult animal tissues, and living materials for human surgery. Larval cells are cells with high division ability that have both the characteristics of embryonic cells and larval cells. They are a type of cells with in vitro culture prospects source of cells. [0004] In vitro culture of scallop trombus larvae cells. Reference: Primary cell culture fromembryos of the Japanese scallop Mizuchopecten yessoensis(Bivalvia)(1991Cytotechnology) [...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/07
Inventor 张志峰晏萌季爱昌毕颖
Owner OCEAN UNIV OF CHINA
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