Chlamys farreri gene chip for detecting toxic effect of tetrabisphenol A

A technology of Chlamys farreri and tetrabromobisphenol, applied in the field of gene chips, can solve the problems of accurate detection of tetrabromobisphenol A's toxic effect on Chlamys farreri, expensive instruments, and high detection cost, and achieve fast detection speed , low detection cost, high detection sensitivity and specificity

Active Publication Date: 2015-12-02
OCEAN UNIV OF CHINA
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Problems solved by technology

Compared with fluorescent quantitative PCR technology, high-throughput sequencing technology is less sensitive and requires expensive instruments, high

Method used

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  • Chlamys farreri gene chip for detecting toxic effect of tetrabisphenol A
  • Chlamys farreri gene chip for detecting toxic effect of tetrabisphenol A
  • Chlamys farreri gene chip for detecting toxic effect of tetrabisphenol A

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Embodiment Construction

[0017] Further illustrate the present invention below by embodiment

[0018] 1. Main instruments:

[0019] Table 1. Main instruments:

[0020]

[0021] Second, the main reagents:

[0022] Table 2. Main reagents:

[0023]

[0024] Three, the concrete steps of the preparation method of gene chip of the present invention are as follows:

[0025] 1. Design specific probes for detection: select 8 specific gene sequences from GenBank and known transcriptome libraries, and use the bioinformatics software PrimerExpress2.0 to design specific probes;

[0026] 2. The probes designed above are synthesized and fixed on the solid-phase carrier nylon membrane according to the designed array layout to form the gene chip of the present invention.

[0027] 4. The method for detecting the toxic effect of tetrabromobisphenol A on Chlamys farreri by using the above-mentioned gene chip, comprising the following steps:

[0028] (a) Preparation of cDNA samples of Chlamys farreri, specifica...

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Abstract

The invention discloses a chlamys farreri gene chip for detecting the toxic effect of tetrabisphenol A. The chip comprises eight types of oligonucleotide probes fixed onto a solid phase carrier, the eight types of oligonucleotide probes are selected from eight gene sequences specific to chlamys farreri, and the eight types of oligonucleotide probes are nucleotide sequences indicated by SEQ ID No. 1-8. The chip can be used for detecting gene differential expression relevant information of eight relevant genes of detoxification metabolism of the chlamys farreri under the coercion of the tetrabisphenol A, and a detection method of the chip comprises the steps of preparing a cDNA sample of the chlamys farreri; conducting fluorescence labeling on the cDNA sample; making the cDNA sample obtained after the fluorescence labeling hybridize with the gene chip; scanning hybridization signals for detecting the gene chip, and obtaining the result. By the application of the chip, the high-throughput detection of the gene expression information can be achieved, the chip further has the advantages of being simple and convenient to operate, efficient and reliable in detection result and capable of saving plenty of manpower, material resources and financial resources and the like, and the wide application prospect is achieved.

Description

technical field [0001] The invention relates to a gene chip for detecting the toxic effect of tetrabromobisphenol A on Chlamys farreri, which is used for detecting changes in expression of related genes caused by tetrabromobisphenol A, and belongs to the technical field of gene chip detection. Background technique [0002] Tetrabromobisphenol A (tetrabisphenolA) is currently the world's largest output and usage of a brominated flame retardant, this flame retardant can be added as a reactive flame retardant (more than 90%) to printed circuit boards and Incorporated into plastic compositions as an additive flame retardant. Due to its widespread use and ability to be released into the environment from products, it is now prevalent in environmental media worldwide. At present, TBBPA has been detected in the atmosphere, water, soil, and organisms. Studies have shown that TBBPA is a potential environmental endocrine disruptor, which has potential persistence, cytotoxicity, immun...

Claims

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Application Information

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IPC IPC(8): C40B40/06C12Q1/68
Inventor 潘鲁青苗晶晶蔡月凤王虹丹
Owner OCEAN UNIV OF CHINA
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