Method for testing toxicity of high algae-laden aquatic organisms

Abstract

The invention discloses a method for testing the toxicity of high algae-laden aquatic organisms. The method comprises the steps that saccharomyces cerevisiae is cultured in a solid medium containing 5% of YPD and 1.5% of agar firstly, after clone is grown out, a YPD fluid medium of 5% is inoculated with monoclone, and overnight shake cultivation is conducted based on 180-250 rpm and 25-58 DEG C; phosphate buffer is added to a culture solution with the OD value of 1.5-1.8 to obtain a blank sample A, a high algae-laden water sample is added to the culture solution with the OD value of 1.5-1.8 to obtain a detection sample B, and the two samples are placed on a mixer for oscillation lasting 2 h; cell washing is conducted on the sample A and the sample B twice by means of PBS, and cell suspension with the concentration of 1*105-1*106 cel ls / mL is prepared; the sample A and the sample B are both added to Annexin V-FITC of 5 mug / mL to be evenly mixed, and then propidium iodide of 5 mug / mL is added to be evenly mixed; reaction is conducted at the room temperature away from light for 5-15 min; flow cytometry detection is conducted within 1 h; the proportion of dead cells, the proportion of living cells and the proportion of apoptotic cells are detected by means of flow cytometry; the apoptosis rate and the death rate of the sample B are compared with those of the blank sample A, so that water toxicity is judged. Early warning technical support is provided for high algae-laden water pollution, so that high water supply quality is guaranteed.

Description

technical field [0001] The present invention relates to the detection method of environmental water body, especially relates to a kind of method for testing the biotoxicity of high algae water, this method uses Saccharomyces cerevisiae to detect, establishes the method for accurately detecting the biotoxicity of high algae water sample, this method finally The result is accurate, stable and reproducible, which is an effective improvement to the existing method. Background technique [0002] At present, the increasingly serious water eutrophication has become one of the global environmental problems. When in summer and autumn, lakes, reservoirs, ponds, etc. sometimes form water blooms due to the large number of blue-green algae, which brings great harm to humans. High-algae water mainly contains blue-green algae. Among the known blue-green algae, more than 40 species can produce toxins, and the same algae strain can also produce many different toxins. Such toxins are water-...

AI Technical Summary

Problems solved by technology

[0004] The purpose of the present invention is to provide a method for testing the biotoxicity of high algae water in order to solve the problems of rough results, low sensitivity, large toxin consumption, and the need for a large number of animal test products in the existing biotoxicity detection method.

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