The invention discloses a method of detecting effect of oral tobacco products upon early apoptosis. The method includes: pretreating a sample, preparing single-cell suspension, calculating cell concentration of the single-cell suspension, inoculating cells, setting groups, setting dosage, adding a test sample, incubating the test sample, collecting cells, washing the cells, adding resuspended cells, mixing Annexin V-FITC and PI (propidium iodide) dye, allowing reaction, adding binding buffer, detecting the test sample after mixing, and performing detecting with a flow cytometer in one hour to obtain an early apoptosis influence rate. Through effective treatment of the sample, optimal setting of detection dosage and correct selection of target cells, the invention allows the effect of the oral tobacco products upon early apoptosis to be accurately and effectively detected.