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Application of fructus mume extract in preparation of medicines or health care products for preventing and treating oral epithelial cell death caused by oral tobacco

A technology of oral epithelial cells and extracts, applied in the field of new tobacco products

Active Publication Date: 2019-08-16
CHINA TOBACCO YUNNAN IND
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The present invention protects its application in the death of oral epithelial cells caused by snus, and no relevant reports have been seen so far

Method used

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  • Application of fructus mume extract in preparation of medicines or health care products for preventing and treating oral epithelial cell death caused by oral tobacco
  • Application of fructus mume extract in preparation of medicines or health care products for preventing and treating oral epithelial cell death caused by oral tobacco
  • Application of fructus mume extract in preparation of medicines or health care products for preventing and treating oral epithelial cell death caused by oral tobacco

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] Example 1: Salivary osmotic pressure of human after ingesting gum-based snus for 1 minute

[0034] (1) 25 healthy volunteers were randomly recruited. The basic information of the volunteers is as follows: Age: 22-24 years old; Gender: 12 males and 13 females;

[0035] (2) Each volunteer collected his saliva in a resting state, centrifuged at 16000rpm for 5min, and the supernatant was used for osmotic pressure measurement.

[0036] (3) After an interval of 1 hour between collecting saliva in a resting state, volunteers used snus (Snus Frost, American Camel product) according to the instructions.

[0037] (4) Collect all the saliva in the mouth of the volunteers within 1 minute after use (do not swallow or spit out saliva during this period).

[0038] (5) Measure the osmotic pressure of saliva, and evaluate the influence of smokeless tobacco use on osmotic pressure of saliva. Measurement results such as figure 1 As shown, the average saliva osmolality of the volunteers...

Embodiment 2

[0040] Example 2: Detection of the effect of hyperosmotic pressure on cell death

[0041] (1) Cell culture and passage: Immortalized human oral epithelial cells hTERT-OME were purchased from Abmgood Company and cultured in MCDB151 medium (Sigma-Aldrich product) containing 1% fetal bovine serum (Hyclone product) by volume. 25cm 2 in a culture bottle. After the cells grow to 80% confluence, discard the culture medium, wash once with PBS, add 0.5-1mL 0.25% trypsin-0.53mM EDTA digestion solution (Sigma-Aldrich product), and digest at 37°C for 1-5 minutes. After the cells become round, discard the digestion solution. Add MCDB151 medium containing 10% fetal bovine serum by volume, blow and resuspend the cells, centrifuge at 600g for 5 minutes, discard the supernatant, and resuspend the cells in 15 ml of the aforementioned MCDB151 medium containing 1% fetal bovine serum by volume , according to the ratio of 1:3 passage. The medium was changed every 2-3 days, and half of the mediu...

Embodiment 3

[0049] The preparation of embodiment 3 ebony extract

[0050] (1) Weigh 100g of ebony plum (dry product, including the core), crush it, and extract it with 500 ml, 300 ml, and 200 ml of ultrapure water, respectively, for 1 hour each time.

[0051] (2) Combine the extracts, refrigerate and centrifuge at 16,000 g at a high speed (4°C, 16,000 g, 30 minutes), keep the supernatant, and discard the precipitate.

[0052] (3) The supernatant was freeze-dried and stored for later use to obtain the ebony extract. The freeze-drying condition was: -30°C, 24 hours.

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Abstract

The invention relates to application of a fructus mume extract in preparation of medicines or health care products for preventing and treating oral epithelial cell death caused by oral tobacco, and belongs to the technical field of new tobacco product research. The invention finds that when a person smokes the oral tobacco, the osmotic pressure of the oral epithelial cell microenvironment is increased, and oral cells are exposed to the hypertonic environment, which leads to cell death; by adding the fructus mume extract to a hypertonic solution, the cell death caused by the hypertonic solutioncan be alleviated. The application of the invention develops a new use for the fructus mume extract, and lays a foundation for the development of protective substances for oral epithelial cell deathcaused by hyperosmosis in the future.

Description

technical field [0001] The invention belongs to the technical field of research on new tobacco products, and in particular relates to the application of an ebony extract in the preparation of medicines or health care products for preventing and treating oral epithelial cell death caused by snus. Background technique [0002] The mouth is the first organ in the human body to come into contact with food and start digestion. In most parts of the oral epithelium, there is no cornified layer (compared to the skin), and no mucus layer formed by mucus (compared to the gastrointestinal tract), so the oral epithelial cells are directly exposed to the external environment. When some of the substances in the body cause death to the epithelial cells, oral discomfort occurs. [0003] Studies have shown that one of the reasons for the death of oral cells is that the osmotic pressure of oral saliva has changed due to external influences. The osmotic pressure of saliva in the resting stat...

Claims

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Application Information

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IPC IPC(8): A61K36/736A61P1/02A23L33/105
CPCA61K36/736A61P1/02A23L33/105A23V2002/00A23V2250/21A23V2200/30
Inventor 杨继向阳高茜米其利管莹杨柳向海英曾婉俐杨光宇李雪梅
Owner CHINA TOBACCO YUNNAN IND
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