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Detection method of influence on streptococcus mutans by oral tobacco products

A technology for Streptococcus mutans and tobacco products, which is applied in the directions of microorganism-based methods, microbial determination/inspection, biochemical equipment and methods, etc., which can solve the problems of less research on the impact of human oral microorganisms, and ensure accuracy and high efficiency. Sex, detection comprehensive and accurate effect

Inactive Publication Date: 2017-12-29
CHINA TOBACCO YUNNAN IND
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The current tobacco products only detect some physical and chemical indicators for the product itself, but there are few studies on the impact of microorganisms in the human oral cavity, especially the research on Streptococcus mutans in the oral cavity has not been involved

Method used

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  • Detection method of influence on streptococcus mutans by oral tobacco products
  • Detection method of influence on streptococcus mutans by oral tobacco products
  • Detection method of influence on streptococcus mutans by oral tobacco products

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0047] A method for detecting the impact of oral tobacco products on Streptococcus mutans, comprising the steps of:

[0048] Step (1), preparing artificial saliva, and adding Streptococcus mutans to artificial saliva, so that the amount of Streptococcus mutans in artificial saliva is 1*10 4 CFU / mL, to obtain artificial saliva containing Streptococcus mutans;

[0049] In step (2), the artificial saliva containing Streptococcus mutans obtained in step (1) is used to extract the snus tobacco product, extracting multiple times, and collecting the extract respectively; the specific method is:

[0050] Inject the artificial saliva containing Streptococcus mutans in step (1) into 0.8 g of the snus tobacco product, and extract by shaking at a constant temperature at 37°C. The shaking speed is 150rpm, and extract twice. Artificial saliva 9mL, each extraction time is 4min, and the extracts are collected separately;

[0051] In step (3), put the extracts collected in step (2) together ...

Embodiment 2

[0070] A method for detecting the impact of oral tobacco products on Streptococcus mutans, comprising the steps of:

[0071] Step (1), preparing artificial saliva, and adding Streptococcus mutans to artificial saliva, so that the amount of Streptococcus mutans in artificial saliva is 1*10 6 CFU / mL, to obtain artificial saliva containing Streptococcus mutans;

[0072] In step (2), the artificial saliva containing Streptococcus mutans obtained in step (1) is used to extract the snus tobacco product, extracting multiple times, and collecting the extract respectively; the specific method is:

[0073] Inject the artificial saliva containing Streptococcus mutans in step (1) into 1.2 g of snus tobacco products, and extract at a constant temperature of 37° C. at a constant temperature of 300 rpm, and extract 3 times. Artificial saliva 10mL, each extraction time is 5min, and the extracts are collected separately;

[0074] In step (3), put the extracts collected in step (2) together w...

Embodiment 3

[0095] A method for detecting the impact of oral tobacco products on Streptococcus mutans, comprising the steps of:

[0096] Step (1), preparing artificial saliva, and adding Streptococcus mutans to artificial saliva, so that the amount of Streptococcus mutans in artificial saliva is 1*10 5 CFU / mL, to obtain artificial saliva containing Streptococcus mutans, numbered as 1# liquid;

[0097] In step (2), the artificial saliva containing Streptococcus mutans obtained in step (1) is used to extract the snus tobacco product, extracting multiple times, and collecting the extract respectively; the specific method is:

[0098] Inject the artificial saliva containing Streptococcus mutans of step (1) into 1 g of snus tobacco products, and extract by shaking at a constant temperature at 37°C. The shaking speed is 200rpm, and extract 3 times. 10mL of saliva, each extraction time is 5min, collect the extract respectively, numbered as 2#, 3#, 4# liquid;

[0099] In step (3), put the extra...

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Abstract

The invention relates to a detection method of influence on streptococcus mutans by oral tobacco products and belongs to the technical field of biological detection. The detection method includes: firstly, preparing artificial saliva containing the streptococcus mutans; then simulating the use process of the oral tobacco products by adopting the artificial saliva according to use characteristics of the oral tobacco products; performing fluorescent quantitation PCR analysis on the streptococcus mutans of the saliva to study change of content of the streptococcus mutans in the saliva before and after the oral tobacco products are used to provide preference to oral health of the oral tobacco products. The streptococcus mutans of different contents can be added to simulate actual situations with the streptococcus mutans of different contents in the oral cavity of different people, and comprehensive guidance is achieved.

Description

technical field [0001] The invention belongs to the technical field of biological detection, and in particular relates to a detection method for the influence of oral tobacco products on Streptococcus mutans. Background technique [0002] Caries is listed by the World Health Organization (WHO) as one of the epidemic infectious diseases that seriously endanger human health. It is a chronic, progressive, and destructive disease that occurs in dental hard tissues under the combined action of bacteria and other factors. Factors that cause caries include: bacteria, food, host, and action time. There are many types of bacteria in the oral cavity, mainly anaerobic bacteria and facultative anaerobic bacteria, including Streptococcus, Lactobacillus and Actinomycetes. Streptococcus mutans has a high detection rate in the oral cavity, close to 90%, and is the main cariogenic bacteria. It destroys the hard tissue of the tooth through adhesion, acid production and acid resistance, thus ...

Claims

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Application Information

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IPC IPC(8): C12Q1/68C12Q1/06C12R1/46
CPCC12Q1/686C12Q1/689C12Q2563/107C12Q2545/114C12Q2561/113
Inventor 高茜管莹杨柳米其利朱洲海徐玉琼陆舍铭夭建华
Owner CHINA TOBACCO YUNNAN IND
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