Method for detecting influence on secretory volume of cell inflammation effectors caused by oral tobacco products

Abstract

The invention discloses a method for detecting influence on secretory volume of cell inflammation effectors caused by oral tobacco products. The method comprises the following steps: (1) pretreating test objects; (2) preparing a human oral cavity keratinocyte single-celled suspension; (3) calculating cell concentration of the human oral cavity keratinocyte single-celled suspension; (4) carrying out cell inoculation on the human oral cavity keratinocyte single-celled suspension; (5) grouping the test objects; (6) setting detection dosage of the test objects; (7) preparing a culture product for the test objects; (8) carrying out incubation on the test objects; (9) collecting incubation products of the test objects; (10) preparing a standard solution; (11) adding samples to be detected; (12) adding antibodies; (13) adding a binding solution; (14) carrying out color development; (15) measuring a light absorption value; (16) determining secretory volume of cell factors. The method disclosed by the invention can be used for accurately and effectively detecting the influence on the secretion of the cell inflammation effectors caused by total granule phase substances of cigarette smoke.

Description

technical field [0001] The invention belongs to the technical field of biological effect evaluation of tobacco products, in particular to a method for detecting the influence of snus products on the secretion of cell inflammatory effect factors. Background technique [0002] As people pay more and more attention to health, higher requirements are put forward for tobacco products, not only to have a better taste, but also to reduce the adverse feelings of the human body as much as possible. Snus products avoid the harm caused by the complex mixture produced by the combustion of traditional cigarette products. At this stage, it has become one of the new directions for foreign tobacco companies to turn from traditional cigarette products. According to the components and morphological characteristics of the products, snus products are divided into traditional Snus-type snus products containing tobacco leaf raw materials and new snus products without tobacco leaf raw materials (s...

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Problems solved by technology

However, there are a large number of primary formulas, and sensory evaluation of all of them is time-consuming and labor-intensive, and the sensory evaluation focuses on screening the style and taste of the product. How to use bio...

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