Method for rapidly detecting photosynthetic rate of phytoplankton based on chlorophyll fluorescence

Abstract

The invention discloses a method for rapidly detecting a photosynthetic rate of phytoplankton based on chlorophyll fluorescence. According to the invention, from the view of energy flow angle of photosynthesis, the chlorophyll fluorescence serves as a probe of photosynthesis, a variable light pulse induction method for phytoplankton chlorophyll fluorescence is provided, the complex photosynthetic energy flow process is segmented, and photosynthetic parameters for leading the photosynthetic energy flow efficiency are obtained in a sectional mode by analyzing the chlorophyll fluorescence kinetic curves under different induction modes. On the basis, according to the biological film energy flow process, a quantitative analysis method for the photosynthetic rate of phytoplankton based on chlorophyll fluorescence is established, the state and growth potential of the photosynthesis of the phytoplankton are rapidly detected in real time, and a method basis is provided for developing an in-situ field measurement technology.

Description

technical field [0001] The invention belongs to the field of environmental science, and in particular relates to a method for rapidly detecting the photosynthetic rate of phytoplankton based on chlorophyll fluorescence. Background technique [0002] Phytoplankton is the primary producer of natural water bodies. Although its photosynthetic tissue accounts for less than 1% of the global total vegetation biomass, its productivity accounts for about 45% of the total plant productivity, and its photosynthetic carbon sequestration accounts for 40% of the global total carbon sequestration. [1] , is essential to maintain the normal functioning of aquatic ecosystems. In recent years, as the degree of eutrophication in my country's water quality has intensified, phytoplankton overgrowth, algal blooms and red tide disasters have occurred frequently, especially since the 1970s, the frequency of algal blooms and red tides has increased by three times every decade The continuous rise has ...

AI Technical Summary

Problems solved by technology

However, most of these traditional photosynthetic rate analysis methods use on-site sampling and off-line analysis, which has long measurement period, low efficiency, poor timeliness and cumbersome procedures

More importantly, the off-line analysis process of the traditional method has changed the growth environment of phytoplankton and affected the physiological state of phytoplankton. It can only obtain the photosynthetic rate in the experimental simulation environment, and it is difficult to quickly and accurately measure the photosynthetic rate of phytoplankton under natural conditions.

Images