Method for detecting influence of oral tobacco product on Porphyromonas gingivalis

A technology for porphyrin gingivalis and tobacco products, which is applied in the field of detection of the influence of oral tobacco products on Porphyromonas gingivalis, can solve the problems of less research on the influence of human oral microorganisms, etc. Detect comprehensive and accurate effects

Inactive Publication Date: 2018-01-26
CHINA TOBACCO YUNNAN IND
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The current tobacco products only detect some physical and chemical indicators for the product itself, but there are few studies on the impact on the microorganisms in the human oral cavity, especially the research on Porphyromonas gingivalis in the oral cavity has not been involved

Method used

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  • Method for detecting influence of oral tobacco product on Porphyromonas gingivalis
  • Method for detecting influence of oral tobacco product on Porphyromonas gingivalis
  • Method for detecting influence of oral tobacco product on Porphyromonas gingivalis

Examples

Experimental program
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Effect test

Embodiment 1

[0046] A method for detecting the impact of oral tobacco products on Porphyromonas gingivalis, comprising the steps of:

[0047] Step (1), preparing artificial saliva, and adding Porphyromonas gingivalis to the artificial saliva, so that the amount of Porphyromonas gingivalis in the artificial saliva is 1*10 4 CFU / mL, to obtain artificial saliva containing Porphyromonas gingivalis;

[0048] In the step (2), the artificial saliva containing Porphyromonas gingivalis obtained in the step (1) is used to extract the snus tobacco products, extracting multiple times, and collecting the extracts respectively; the specific method is:

[0049] Inject the artificial saliva containing Porphyromonas gingivalis in step (1) into 0.8 g of snus tobacco products, extract at a constant temperature of 37° C., and extract twice at a shaking speed of 150 rpm. The artificial saliva of Porphyromonas was 9mL, and the extraction time was 4min each time, and the extracts were collected separately;

[...

Embodiment 2

[0069] A method for detecting the impact of oral tobacco products on Porphyromonas gingivalis, comprising the steps of:

[0070] Step (1), preparing artificial saliva, and adding Porphyromonas gingivalis to the artificial saliva, so that the amount of Porphyromonas gingivalis in the artificial saliva is 1*10 6 CFU / mL, to obtain artificial saliva containing Porphyromonas gingivalis;

[0071] In the step (2), the artificial saliva containing Porphyromonas gingivalis obtained in the step (1) is used to extract the snus tobacco products, extracting multiple times, and collecting the extracts respectively; the specific method is:

[0072] Inject the artificial saliva containing Porphyromonas gingivalis in step (1) into 1.2 g of snus tobacco products, and extract at a constant temperature of 37° C. at a shaking speed of 300 rpm, and extract 3 times. The artificial saliva of Porphyromonas was 10mL, and the extraction time was 5min each time, and the extracts were collected separatel...

Embodiment 3

[0093] A method for detecting the impact of oral tobacco products on Porphyromonas gingivalis, comprising the steps of:

[0094] Step (1), preparing artificial saliva, and adding Porphyromonas gingivalis to the artificial saliva, so that the amount of Porphyromonas gingivalis in the artificial saliva is 1*10 5 CFU / mL, the artificial saliva containing Porphyromonas gingivalis was obtained, and the number was 1# liquid;

[0095] In the step (2), the artificial saliva containing Porphyromonas gingivalis obtained in the step (1) is used to extract the snus tobacco products, extracting multiple times, and collecting the extracts respectively; the specific method is:

[0096] Inject the artificial saliva containing Porphyromonas gingivalis in step (1) into 1 g of snus tobacco products, and extract by shaking at a constant temperature at 37°C. The shaking speed is 200rpm, and extract 3 times. The artificial saliva of Porphyromonas is 10mL, and the extraction time is 5min each time, ...

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Abstract

The invention relates to a method for detecting influence of an oral tobacco product on Porphyromonas gingivalis and belongs to the technical field of biological detection. According to the method, firstly, artificial saliva containing the Porphyromonas gingivalis is prepared, aiming at use characteristics of oral products, the artificial saliva is adopted to simulate a use process of the oral tobacco product and fluorescent quantitative PCR analysis is performed on content of the Porphyromonas gingivalis in the saliva, so that content change of the Porphyromonas gingivalis in the saliva before and after use of the oral tobacco product is investigated, and reference is provided for mouth health of the oral tobacco product. Actual conditions of different content of the Porphyromonas gingivalis in oral cavities of different people can be simulated by adding different content of the Porphyromonas gingivalis, and omnibearing guidance is provided.

Description

technical field [0001] The invention belongs to the technical field of biological detection, and in particular relates to a detection method for the influence of oral tobacco products on Porphyromonas gingivalis. Background technique [0002] According to the results of the third national oral health epidemiological survey, the prevalence of chronic periodontitis (CP) has reached over 80%, and it is the main cause of tooth loss in people over 35 years old. Modern medical research shows that chronic periodontitis is mostly caused by the progressive destruction of periodontal tissue caused by factors such as poor oral hygiene and occlusal trauma. In the early stage, it usually manifests as bad breath, red and swollen gums, and bleeding. If left untreated for a long time, it will lead to loose teeth , Alveolar bone destruction, or even tooth extraction, in addition it is also related to the functions of important organs such as the heart, lungs, and kidneys. Periodontal tissue...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6851C12Q1/06C12R1/01
Inventor 高茜段沅杏赵伟杨继田永峰管莹米其利朱洲海夭建华
Owner CHINA TOBACCO YUNNAN IND
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