Method for detecting influence of oral tobacco product on Porphyromonas gingivalis
A technology for porphyrin gingivalis and tobacco products, which is applied in the field of detection of the influence of oral tobacco products on Porphyromonas gingivalis, can solve the problems of less research on the influence of human oral microorganisms, etc. Detect comprehensive and accurate effects
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0046] A method for detecting the impact of oral tobacco products on Porphyromonas gingivalis, comprising the steps of:
[0047] Step (1), preparing artificial saliva, and adding Porphyromonas gingivalis to the artificial saliva, so that the amount of Porphyromonas gingivalis in the artificial saliva is 1*10 4 CFU / mL, to obtain artificial saliva containing Porphyromonas gingivalis;
[0048] In the step (2), the artificial saliva containing Porphyromonas gingivalis obtained in the step (1) is used to extract the snus tobacco products, extracting multiple times, and collecting the extracts respectively; the specific method is:
[0049] Inject the artificial saliva containing Porphyromonas gingivalis in step (1) into 0.8 g of snus tobacco products, extract at a constant temperature of 37° C., and extract twice at a shaking speed of 150 rpm. The artificial saliva of Porphyromonas was 9mL, and the extraction time was 4min each time, and the extracts were collected separately;
[...
Embodiment 2
[0069] A method for detecting the impact of oral tobacco products on Porphyromonas gingivalis, comprising the steps of:
[0070] Step (1), preparing artificial saliva, and adding Porphyromonas gingivalis to the artificial saliva, so that the amount of Porphyromonas gingivalis in the artificial saliva is 1*10 6 CFU / mL, to obtain artificial saliva containing Porphyromonas gingivalis;
[0071] In the step (2), the artificial saliva containing Porphyromonas gingivalis obtained in the step (1) is used to extract the snus tobacco products, extracting multiple times, and collecting the extracts respectively; the specific method is:
[0072] Inject the artificial saliva containing Porphyromonas gingivalis in step (1) into 1.2 g of snus tobacco products, and extract at a constant temperature of 37° C. at a shaking speed of 300 rpm, and extract 3 times. The artificial saliva of Porphyromonas was 10mL, and the extraction time was 5min each time, and the extracts were collected separatel...
Embodiment 3
[0093] A method for detecting the impact of oral tobacco products on Porphyromonas gingivalis, comprising the steps of:
[0094] Step (1), preparing artificial saliva, and adding Porphyromonas gingivalis to the artificial saliva, so that the amount of Porphyromonas gingivalis in the artificial saliva is 1*10 5 CFU / mL, the artificial saliva containing Porphyromonas gingivalis was obtained, and the number was 1# liquid;
[0095] In the step (2), the artificial saliva containing Porphyromonas gingivalis obtained in the step (1) is used to extract the snus tobacco products, extracting multiple times, and collecting the extracts respectively; the specific method is:
[0096] Inject the artificial saliva containing Porphyromonas gingivalis in step (1) into 1 g of snus tobacco products, and extract by shaking at a constant temperature at 37°C. The shaking speed is 200rpm, and extract 3 times. The artificial saliva of Porphyromonas is 10mL, and the extraction time is 5min each time, ...
PUM
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com