Microfluidic chip for cell culture, and dynamic culture device and method

A microfluidic chip and cell culture technology, applied in the field of biomedical applications, can solve the problems of low survival rate of embryonic cells, inability to provide a simulated intrauterine environment, and inability to achieve efficient culture of embryonic cells, and achieve the effect of easy diffusion

Active Publication Date: 2015-02-25
SHENZHEN GRADUATE SCHOOL TSINGHUA UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

The technical effect that this patented technology solves by providing stable supply of these substances for both developing fetuses during pregnancy or growing them into babies' bodies after childbirth without causing any harm from excessive exposure to other chemicals used later on.

Problems solved by technology

The technical problem addressed in this patented method relates to improving the efficiency at developing an early embryonal cell (ESC) into adulthood due to its unique properties such as being able to grow quickly without losing their ability to develop new organs or differentiate them correctly when implanted in humans.

Method used

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  • Microfluidic chip for cell culture, and dynamic culture device and method
  • Microfluidic chip for cell culture, and dynamic culture device and method
  • Microfluidic chip for cell culture, and dynamic culture device and method

Examples

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Embodiment 1

[0029] Please see figure 1 It is a schematic diagram of the overall connection relationship of each part of the first embodiment of the present invention, a microfluidic chip-based embryonic cell in vitro dynamic culture device, including a microfluidic chip 1, a nutrient pool 2, an outlet storage pool 3 and a test bench 4. The microfluidic chip 1 is a self-designed embryonic cell culture structure that provides the space, nutrients, and mechanical stimulation required for embryonic cell culture. Nutrient pool 2 is the inlet reservoir for the culture medium on the microfluidic chip, and the outlet reservoir 3 is the outlet reservoir for the embryonic cell culture fluid. The driving force of the liquid flow on the microfluidic chip 1 comes from the nutrient pool 2 And the liquid level difference of outlet reservoir 3. Experiment bench 4 comprises base 12, object stage 13, support column 16 and height adjustment device 14, wherein, object stage 13 and support column 16 are con...

Embodiment 2

[0037] The present invention also proposes a cultivation method utilizing the above cultivation device, comprising the steps of:

[0038] S1. Add cell solution into the nutrient pool 2, leave the outlet reservoir 3 empty, the liquid level difference between the nutrient pool 2 and the outlet reservoir 3 drives the solution to flow in the microfluidic chip 1, and the cell capture unit 9 captures a single cell ;

[0039] S2, add culture medium in the outlet liquid storage pool 3 and adjust the height adjustment device 14, make the liquid level in the nutrient pool 2 and the outlet liquid storage pool 3 equal, transfer the embryonic cell in vitro dynamic culture device to the incubator and set the culture time;

[0040] S3. Remove the culture solution in the nutrient pool 2 and the outlet storage tank 3, empty the outlet storage tank 3, and re-add a set amount of culture solution in the nutrition pool 2;

[0041] S4. Adjust the liquid level difference between the nutrient pool ...

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Abstract

The invention discloses a microfluidic chip for cell culture, and a dynamic culture device and method. The microfluidic chip comprises a chip inlet, a chip outlet, a channel and a cell trapping structure, wherein the cell trapping structure comprises a plurality of cell trapping units; the channel comprises a first channel and a second channel which are mutually communicated; the first channel is communicated with the second channel respectively through a first connected region and a second connected region; each cell trapping unit comprises a cell trapping unit inlet and a cell trapping unit outlet; the cell trapping unit inlet is positioned on the side of the first channel; the cell trapping unit outlet is positioned on the side of the second channel; and the cell trapping unit outlet forms the second connected region. The embryo cell in-vitro dynamic culture device comprises the microfluidic chip, and can be used for performing simple and efficient in-vitro culture on the embryo cells.

Description

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Claims

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Application Information

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Owner SHENZHEN GRADUATE SCHOOL TSINGHUA UNIV
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