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Microfluidic array chip for cell capture

An array chip, microfluidic technology, applied in enzymology/microbiology devices, biochemical instruments, biochemical equipment and methods, etc., to achieve the effect of ensuring sealing, simple operation, simple and efficient use process

Inactive Publication Date: 2013-11-13
PEOPLES LIBERATION ARMY ORDNANCE ENG COLLEGE
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] Domestic research in this field is still in its infancy

Method used

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  • Microfluidic array chip for cell capture
  • Microfluidic array chip for cell capture
  • Microfluidic array chip for cell capture

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Experimental program
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Embodiment Construction

[0019] The present invention is described in detail as follows in conjunction with accompanying drawing:

[0020] The overall structure of the chip is as figure 1 As shown, it includes a silicon material microfluidic tube substrate (1), a glass material upper cover (2), cell capture micropillar arrays (3) evenly arranged at a certain distance, and a 4-60 μm deep microfluidic tube on a substrate (4 ), 120 μm deep upper cover microfluidic pipeline (5), drainage tube inlet and outlet (6), drainage rubber tube (7).

[0021] The silicon material microfluidic tube substrate (1) has a structure such as figure 2 As shown, it is divided into silicon wafers, and its size is about 31mm×24mm×0.3mm.

[0022] The structure of the soda-lime glass upper cover (2) is as follows image 3 As shown, it is made of ordinary soda-lime glass, and its size is about 31mm×24mm×1.2mm.

[0023] The cell-capturing micropillar array (3) arranged uniformly at a certain interval has a structure such as ...

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Abstract

The invention relates to a microarray-type structure chip adopting micro-flow pipes and taking advantage of diameter difference of different types of cells, and micro column arrays with different intervals and different sizes are etched in the micro-flow pipes. The chip structure is formed by up-down bonding of a silicon Si material negative-film substrate and a soda-lime glass upper cover. According to the different intervals of the column micro structure, the microarray-type structure chip can be divided into two series: variable interval and fixed interval; and according to the different sizes of the column micro structure, the microarray-type structure chip can be divided into a plurality of types: ranging from 2-30 [mu] m. The microarray-type structure chip is characterized in that: when a liquid fluid containing the different types of cells flows through the chip micro structure, and passes through the column microarrays with different intervals and diameters, different types (different sizes) of cells can be captured by different micro column array structures, and retained on the different micro column arrays with different micro structures in the pipes, and the captured results can be observed and analyzed by used of a microscope. The microarray-type structure chip has the characteristics of micro volume, flexible and customizable structure, no moving parts and the like, and can be widely applied to capture and analysis and other fields of various cancer cells in the blood, and comprehensive analysis and measuring and calculating determine of the cell type and quantity in the blood of patients can be conducted through use of different kinds of chips.

Description

technical field [0001] The invention belongs to the field of biological cell detection in a fluid, in particular to a design of a cell capture chip utilizing a micro-column array structure distributed in a micron-scale micro-flow tube. Background technique [0002] The microfluidic chip originally originated in the field of analytical chemistry. It is a kind of micro-cavity, micro-pipeline network structure and other functional units made on a substrate of several square centimeters by using fine processing technology to realize the preparation and injection of micro-sample. , reaction, separation and detection in one of the rapid, efficient, low-cost micro-analysis experimental device. [0003] The use of microfluidic chips for blood sample analysis is a scientific research hotspot that has emerged in recent years. For example, a team led by Mehmet Toner, a professor of biomedical engineering at Harvard Medical School, designed a small device in 2011 that could detect canc...

Claims

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Application Information

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IPC IPC(8): C12M1/34
Inventor 王广龙李其昌高凤岐卢江雷高敏陈建辉乔中涛张延生杨叶舟魏忠林井建辉
Owner PEOPLES LIBERATION ARMY ORDNANCE ENG COLLEGE
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