The invention discloses a three-axis micro-gyroscope based on a tuning fork driving effect, which comprise an upper-layer gyroscope structure and a lower-layer glass substrate, the upper-layer gyroscope structure comprises a left mechanical structure, a right mechanical structure, a middle mechanical structure, an upper crossbeam, a lower crossbeam, short beams, and anchor points, a turning mechanism based on tuning fork driving is designed in the middle mechanical structure, X-axis simple harmonic driving and Y-axis simple harmonic driving of sensitive weights of the middle mechanical structure are realized by the X-axis unidirectional driving of the left mechanical structure and the right mechanical structure, and thereby the consistency of driving frequencies between different axes is ensured. When angular velocity is inputted from outside, the sensitive weights receives Coriolis force to shift. By adopting a double-sensitive weight design and variable-interval capacitance differential detection, the three-axis micro-gyroscope can inhibit the common-mode interference of temperature, structural stress, acceleration and the like; the structure of the three-axis micro-gyroscope issymmetrically arranged, the stability is good, and the anti-interference capability is high.