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Circulating tumor cell detection kit and application thereof

A detection kit and tumor cell technology, applied in the field of in vitro diagnosis, can solve the problems of low activity of CTCs cells, inability to separate cells, low capture efficiency of CTCs, etc., and achieve the effect of high-efficiency enrichment and detection

Inactive Publication Date: 2016-07-20
杭州华得森生物技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This method has a low capture efficiency for CTCs, and the captured CTCs have low cell activity, which cannot be used for later cell culture and cell separation, and can be used for downstream technology research
[0006] Flow cytometry (FCM) detection method is relatively simple, with high accuracy and sensitivity, and can quantitatively detect CTCs in the peripheral blood of cancer patients. In terms of the cell morphology of tumor cells, specific immune cell staining under the microscope is still needed to make up for the shortcomings of the method

Method used

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  • Circulating tumor cell detection kit and application thereof
  • Circulating tumor cell detection kit and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0064] Example 1: Detection of circulating tumor cells in cholangiocarcinoma

[0065] 1 Circulating Tumor Cell Isolation

[0066] 1.1 Equilibrate the density gradient centrifugate to room temperature.

[0067] 1.2 Add 3mL of density gradient centrifugation solution into each density gradient centrifuge tube.

[0068] 1.3 Place the tube containing 3mL density gradient centrifugation solution in a centrifuge, centrifuge at 1000×g for 30s at room temperature, and centrifuge the density gradient centrifugation solution below the porous barrier.

[0069] 1.4 Dilute whole blood samples of cholangiocarcinoma patients with 1×PBS at a ratio of 1:1.

[0070] 1.5 Carefully inject 4mL of the diluted whole blood sample into the density gradient centrifuge tube (containing the density gradient centrifuge).

[0071] 1.6 Place the density gradient centrifuge tube (including the density gradient centrifugate) containing the whole blood dilution sample in the swinging bucket rotor of the cen...

Embodiment 2

[0100] Example 2: Detection of circulating tumor cells and circulating tumor stem cells in non-small cell lung cancer

[0101] 1 Isolation of circulating tumor cells

[0102] 1.1 Equilibrate the density gradient centrifugate to room temperature.

[0103] 1.2 Add 3mL of density gradient centrifugation solution into each density gradient centrifuge tube.

[0104] 1.3 Place the tube containing 3mL density gradient centrifugation solution in a centrifuge, centrifuge at 1000×g for 30s at room temperature, and centrifuge the density gradient centrifugation solution below the porous barrier.

[0105] 1.4 Dilute whole blood samples of cholangiocarcinoma patients with 1×PBS at a ratio of 1:1.

[0106] 1.5 Carefully inject 4mL of the diluted whole blood sample into the density gradient centrifuge tube (containing the density gradient centrifuge).

[0107] 1.6 Place the density gradient centrifuge tube (including the density gradient centrifugate) containing the whole blood dilution sam...

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Abstract

The invention relates to a circulating tumor cell detection kit and application thereof. The circulating tumor cell detection kit comprises a chip, sample diluent, a cell separation fluid, a cell trapping agent, a cell cleaning solution, a cell fixing agent, antibody diluent, a cell penetration agent and a fluorescence dye. The application method of the circulating tumor cell detection kit mainly comprises the several steps of separation of circulating tumor cells, trapped antibody coating, trapping of the circulating tumor cells and immuno-fluorescent staining of the circulating tumor cells. The circulating tumor cell detection kit is mainly used for tumor prognosis, reappear, metastasis, curative effect monitoring, early diagnosis and early warning, monitoring of curative effect and tumor progression situation, and utilizes the molecular subtyping, screening specificity and other characteristics of the circulating tumor cells to conduct targeted research on circulating tumor cell sensitive drug so as to supplement tumor metastasis, relapse and drug resistance mechanisms and guide individualized medical treatment.

Description

technical field [0001] The invention relates to a detection kit for circulating tumor cells and its application, which is used for clinical auxiliary diagnosis of tumor prognosis, recurrence, metastasis, curative effect monitoring, etc., and belongs to the technical field of in vitro diagnosis. Background technique [0002] Circulating Tumor Cells (CTCs) refer to tumor cells that are released into the peripheral blood circulation from solid tumors or metastases spontaneously or due to diagnostic and therapeutic procedures, and are a general term for various types of tumor cells that exist in peripheral blood. [0003] Malignant tumors will spread to other organs of the body through blood transmission, and tumor metastasis is the main cause of death of tumor patients. Tumor cells invade the surrounding tissues of primary tumor cells, enter the blood and lymphatic vasculature, form circulating tumor cells (CTCs), and transport to distant tissues, exudate, adapt to the new micr...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/574G01N33/533
CPCG01N33/574G01N33/533G01N33/56961
Inventor 张开山苏广宇宁宁
Owner 杭州华得森生物技术有限公司
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