Microfluidic cell-sorting chip integrated with single-cell capture

Abstract

The invention discloses a microfluidic cell-sorting chip integrated with single-cell capture, belongs to the field of microfluidic chips and provides a microfluidic chip capable of being used for detection and diagnosis of leukemia without damaging the activity of leukemia cells. The microfluidic cell-sorting chip is characterized in that the microfluidic cell-sorting chip comprises a substrate and a cell capturing device, wherein an electrode pair is sputtered on the substrate; the cell capturing device is provided with a sample inlet and two sample outlets; the cell capturing device comprises an upper capturing layer and a lower capturing layer which are aligned and bonded to form a cell capturing array and a microfluidic channel; the cell capturing array comprises a plurality of isomorphous single-cell capturing structures; each single-cell capturing structure comprises a U-shaped column and a cylinder; the sample inlet is connected with the head end of the microfluidic channel after penetrating through the upper capturing layer; the microfluidic channel is divided into two paths, and each path is connected with the front end of one cell capturing array; the microfluidic channelconnected with the rear end of the cell capturing array is connected with the sample outlets penetrating through the upper capturing layer. The microfluidic cell-sorting chip can be used for the field of cell sorting.

Description

technical field [0001] The invention discloses a microfluidic cell sorting chip integrated with single cell capture, belonging to the technical field of microfluidic chips. Background technique [0002] Cell separation is an important part of modern biology and one of the key steps in cell research, which is very important in disease diagnosis and single cell analysis. At present, particle separation methods are divided into active and passive separation. The current separation technologies include magnetic excitation cell separation technology, fluorescence excitation cell separation technology, centrifugal separation technology and dielectrophoresis separation technology. In these methods, the cells separated by dielectrophoresis without labeling can be further cultured for subsequent biological analysis, and it also has the advantages of low cost, high efficiency, and non-invasiveness, and can separate different types of particles, such as DNA, protein, virus and bacteria...

AI Technical Summary

Problems solved by technology

[0005] The microfluidic cell sorting chip integrated with single cell capture of the present invention overcomes the shortcomings of high cost and low yield of the prior art, and provides a microfluidic chip that can be used in the detection and diagnosis of leukemia without destroying the activity of leukemia cells

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