Method used for extracting antioxidative active polypeptides from squid nidamental glands

A technology of antioxidant activity and squid, which is applied in the field of extracting antioxidant active polypeptides from squid ovary glands, can solve the problems of unreasonable and high-value development and people's neglect, and achieve good lipid antioxidant capacity and easy Digestion and absorption, simple effect of extraction

Inactive Publication Date: 2018-07-13
磐安富晟食品科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

According to relevant research reports, most of the gonads of marine organisms are rich in nutrients and often contain a variety of active substances, but this part of the research is often ignored by people, and has not been developed reasonably and with high value, and it is urgent to obtain reasonable and sufficient utilization

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0013] The method for extracting antioxidant active polypeptides from squid ovary glands includes pretreatment, double enzyme hydrolysis, and separation and purification. The specific steps are:

[0014] 1) Pretreatment: Wash squid ovary glands with clean water, drain, chop them up and then mash them into pieces, add them into NaOH solution with a concentration of 1.3‰ according to the ratio of material to liquid at 1:7, and the NaOH solution contains 0.4% microwave Modified activated carbon was ultrasonically soaked at 200W ultrasonic power and 2°C for 4h, washed with water until neutral, and finally added 12% isopropanol solution to the squid eggs at a material-to-liquid ratio of 1:20. Soak and degrease at 2°C for 15 hours, wash and drain with distilled water, and set aside. Squid ovary glands contain a lot of miscellaneous proteins and fats, so pre-treatment is required to remove these miscellaneous proteins, fats and pigments, etc., which can reduce this The effect of simi...

Embodiment 2

[0019] The method for extracting antioxidant active polypeptides from squid ovary glands includes pretreatment, double enzyme hydrolysis, and separation and purification. The specific steps are:

[0020] 1) Pretreatment: Wash squid ovale glands with water, drain, chop and then mash the tissues, and add it to the NaOH aqueous solution with a concentration of 0.8‰ according to the ratio of material to liquid at 1:9, and the NaOH aqueous solution contains 0.6% microwave Modified activated carbon was ultrasonically soaked for 2 hours at an ultrasonic power of 150W and a temperature of 5°C, washed with water until it was neutral, and finally, an 8% isopropanol solution was added to squid eggs at a material-to-liquid ratio of 1:24. Soak and degrease at a temperature of 5°C for 10 hours, wash and drain with distilled water, and set aside. The activated carbon used in the above pretreatment is microwave modified activated carbon. Keep it for 40 minutes, filter out the water, rinse rep...

Embodiment 3

[0024] The method for extracting antioxidant active polypeptides from squid ovary glands includes pretreatment, double enzyme hydrolysis, and separation and purification. The specific steps are:

[0025] 1) Pretreatment: Wash the squid ovary glands with water, drain, chop them up and then mash them into pieces, and add them to the NaOH solution with a concentration of 1.0‰ according to the ratio of material to liquid at 1:8, and the NaOH solution contains 0.5% microwave Modified activated carbon was ultrasonically soaked for 3 hours at an ultrasonic power of 180W and a temperature of 4°C, washed with water until it was neutral, and finally a 10% isopropanol solution was added to the squid eggs at a material-to-liquid ratio of 1:22. Soak and degrease at 4°C for 12 hours, wash and drain with distilled water, and set aside. The activated carbon used in the above pretreatment is microwave modified activated carbon. The preparation method is: the activated carbon is boiled in a 5% h...

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PUM

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Abstract

The invention discloses a method used for extracting antioxidative active polypeptides from squid nidamental glands. The method comprises following steps: squid nidamental glands are subjected to supersonic wave immersion in a NaOH aqueous solution, water washing is carried out until pH value of an obtained product is 7, an isopropanol solution is adopted for immersion degreasing, and washing anddraining are carried out; a glycine-NaOH buffer is added into the squid nidamental glands obtained via the above pretreatment, an obtained mixture is mixed to be unidorm, and alkali protease and neutral protease are adopted for hydrolysis so as to obtain an enzymatic hydrolysate; the enzymatic hydrolysate is subjected to grading using an ultrafilter membrane so as to obtain an ultra-filtered enzymatic hydrolysate, and the ultra-filtered enzymatic hydrolysate is subjected to gel column chromatography, ion exchange resin chromatography, and reversed-phase high-performance liquid chromatography purification so as to obtain the antioxidative active polypeptides. The beneficial effects of the method are that: the extraction rate is high; the method is simple; industrialized production is convenient to realize; safety is higher; the added value of squid nidamental glands is increased; the obtained antioxidative active polypeptides is white in color, possesses no fishiness, and is high in heat stability.

Description

technical field [0001] The invention relates to the technical field of deep processing of fish products, in particular to a method for extracting antioxidant active polypeptides from squid ovary glands. Background technique [0002] Squid, although it is customary to call them fish, is actually not a fish, but a mollusk that lives in the ocean. Squid belongs to Molluscs, Cephalopoda, Calamariidae, commonly known as calamari. The body color is pale, the body is conical, the head is big, with light brown spots, and there are 10 tentacles in front. They often swim in groups in the ocean about 20 meters deep. It is often active in the middle and upper layers of shallow seas, with a vertical movement range of more than 100 meters. The meat is tender and the flavor is similar to abalone, but the price is very low, so it is called "poor man's abalone". Westerners call squid "devil fish" because of its dark and volatile skin; Spaniards eat more squid, and they process squid into ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P21/06C07K1/36C07K1/34C07K1/20C07K1/18C07K1/16
CPCC07K1/16C07K1/18C07K1/20C07K1/34C07K1/36C12P21/06
Inventor 谢剑兵杨艳
Owner 磐安富晟食品科技有限公司
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