Urine exfoliated tumor cell micro-fluidic chip detection technology aiming at urothelium carcinoma

Abstract

The invention belongs to the field of biological fluid detection in vitro, and concretely relates to a urine exfoliated tumor cell micro-fluidic chip detection technology aiming at urothelium carcinoma. The micro-fluidic chip comprises a sample inlet, a cell capturing area and a sample outlet which are connected sequentially, wherein the cell capturing area is provided with a plurality of cell sorting devices; each cell sorting device is formed by three columnar bulges arranged in an arc shape; a gap exists between every two columnar bulges; an arc-shaped opening is used as a liquid flow inlet; gaps at two sides of each middle columnar bulge are used as liquid flow outlets which are symmetrically distributed. According to the urine exfoliated tumor cell micro-fluidic chip detection technology provided by the invention, various cells in urine are separated and captured, multiple downstream cytology staining methods can be compatible for realizing cell recognition, the detection accuracy of urine exfoliated tumor cells in human urine is improved, the dependency on subjective experiences of pathology doctors in a conventional method is avoided, and the whole process is noninvasive. In addition, a lossless cell capturing and recovering manner lays a foundation on downstream molecular biology analysis.

Description

technical field [0001] The invention belongs to the field of extracorporeal detection of biological bodies, and in particular relates to a microfluidic chip detection technology for urinary exfoliation tumor cells for urothelial cancer. Background technique [0002] 1. Active clinical technology-urinary exfoliation cytology examination: Urinary exfoliation cytology examination (Cytology) is currently a commonly used clinical method for the detection of urothelial carcinoma. In order to find tumor cells, this method relies on the subjective observation of pathologists on smears (judging cells based on factors such as cell nuclei, chromatin, and cytoplasm), which mainly has the following disadvantages: [0003] (1) The detection sensitivity is low, generally only 30-50%. [0004] (2) The film reading depends entirely on the subjective experience of pathologists, and the judgment results of different pathologists may be inconsistent. [0005] (3) The dyeing process is cumbers...

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Problems solved by technology

[0005] (3) The dyeing process is cumbersome, with high manual participation and high work intensity

[0008] (1) It is an invasive examination, and the patient suffers a lot;

[0009] (2) The tumor can only be observed when it develops to the naked eye, which is not conducive to early diagnosis.

[0013] (2) The pore diameter of the filter membrane is single, 7.5um; however, the cell composition in the urine of patients with urothelial carcinoma is complex, in addition to tumor cells, there are also normal exfoliated urothelial cells, white blood cells, red blood cells, etc., these The cell sizes are inconsistent. If a single pore size is used, the cell components in the field of view will still be confused, which will interfere with subsequent judgment and analysis;

[0014] (3) It is impossible to perform real-time imaging, monitoring, and regulation of the cell capture process, for example: (a) in the case of clogging of the filter membrane caused by excess cells, the process cannot be terminated in time; (b) the cell damage is seriously damaged when the flow rate is too high In the case of , it is impossible to control the flow rate in real time;

[0015] (4) Limited by the maximum load capacity, when the number of cells is too high, all the filter holes are occupied, and the filter membrane will fail immediately; the subsequent cells will all block the filter membrane, resulting in confusion of the field of view, making it difficult to proceed to the next step. Interpretation of levels

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