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Microfluidic chip for enriched capturing of target cells with different sizes

A microfluidic chip and cell technology, applied in enzymology/microbiology devices, special-purpose bioreactors/fermenters, biochemical instruments, etc., can solve the conditions that cannot be achieved for the treatment, separation and purification of circulating tumor cells of different sizes Single, circulating tumor cell loss and other problems, to achieve the effect of improving sample injection fluency and throughput, high capture efficiency, and large structure size

Active Publication Date: 2019-08-06
XI AN JIAOTONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the separation and purification conditions in this chip are single, and the processing of circulating tumor cells of different sizes cannot be realized; at the same time, the separation and purification conditions in the chip are mainly set for a certain size, which will easily lead to the loss of circulating tumor cells of other sizes, affecting the separation and purification. efficiency

Method used

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  • Microfluidic chip for enriched capturing of target cells with different sizes
  • Microfluidic chip for enriched capturing of target cells with different sizes
  • Microfluidic chip for enriched capturing of target cells with different sizes

Examples

Experimental program
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Effect test

Embodiment 1

[0051] Example 1, such as figure 1 As shown, the expression capture area is used, a microfluidic chip that enriches and captures target cells of different specifications and sizes, and is composed of a chip body connected to a negative film. The chip body is provided with a chip chamber, and the chip chamber is provided with a sample inlet 100, Injection separation column 200, enrichment screening area, expression capture area, non-target cell waste liquid outlet 610 and target cell waste liquid outlet 620;

[0052] The blood sample to be processed enters the chip chamber from the sample inlet 100, and the blood sample is divided into two by the sample injection isolation column 200 and enters the enrichment and screening area. The cell clusters, cell clusters and single cells of the target cells with the largest diameter are enriched and screened in turn to become the target cell liquid, and the remaining blood cell liquid becomes non-target cell waste liquid, which is discha...

Embodiment 2

[0069] Example 2, such as figure 2 As shown, the size capture area is used, a microfluidic chip for enriching and capturing target cells of different specifications and sizes, which is composed of a chip body connected to a negative film. A chip chamber is provided in the chip body, and a sample inlet 100, Injection isolation column 200, enrichment screening area, size capture area, non-target cell waste liquid outlet 610 and target cell waste liquid outlet 620;

[0070] The blood sample to be processed enters the chip chamber from the sample inlet 100, and the blood sample is divided into two by the sample injection isolation column 200 and enters the enrichment and screening area. The cell clusters, cell clusters and single cells of the target cells with the largest diameter are enriched and screened in turn to become the target cell liquid, and the remaining blood cell liquid becomes non-target cell waste liquid, which is discharged from the chip chamber through the non-ta...

experiment example 1

[0087] Experimental Example 1: The microfluidic chip of Example 2 was used to detect the influence of sample injection flux on the capture efficiency of the microfluidic chip.

[0088] 5ml of blood from healthy volunteers was added to 20, 30 and 100 cell clusters, cell clusters and single cells of human breast cancer cell MCF-7 (HTB-22TM, human breast cancer cell line, ATCC, U.S.), respectively, with PBS buffer Diluted at a volume ratio of 1:10 (blood: PBS buffer solution), the diluted solution is the blood sample.

[0089] In this experiment example, a total of 5 groups of experiments were carried out, and each group of experiments was repeated 3 times to ensure the reliability of the experimental results. Experimental groups 1-5 respectively correspond to fluxes of 0.6ml / h, 1.0ml / h, 3.0ml / h, and 5.0ml / h and 10.0ml / h.

[0090] Use the syringe pump LSP01-1A to pump the blood sample into the chip chamber, and obtain target cells of different sizes through the preliminary enri...

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Abstract

The invention relates to a microfluidic chip for enriched capturing of target cells with different sizes, which is composed of a chip body and a negative film. A chip chamber in the chip body is provided with a sample inlet, an injection isolation column, an enrichment screening area, a capture area, and a non-target cell waste liquid outlet and a target cell waste liquid outlet; a blood sample to-be-treated enters the chip chamber from the sample inlet, and the blood sample is divided into two into the enrichment screening area through the injection isolation column, and the enrichment screening area sequentially enriches and screens the cell mass of the target cell with a diameter larger than the critical separation diameter, cell cluster and single cells, the target cell liquid is obtained, and the remaining blood cell liquid is discharged out of the chip chamber from the non-target cell waste liquid outlet; the target cell liquid enters the capture area, the capture area captures the target cell, and the remaining cell liquid is discharged out of the chip chamber from the target cell waste liquid outlet. The microfluidic chip is suitable for practical applications, can achievehigh capture rate, high purity and high throughput, and improves performance of existing microfluidic chips.

Description

technical field [0001] The invention relates to the technical field of biomedical microfluidic chips, in particular to a microfluidic chip for enriching and capturing target cells of different sizes. Background technique [0002] The concept of circulating tumor cells was proposed by Australian scholar Ashworth as early as 1869. They are tumor cells free in the blood circulation system. important factor in the lethal mechanism. Therefore, early detection of circulating tumor cells has important clinical significance. [0003] According to reports, in the mouse tumor model of metastatic breast cancer, the adhesion group of circulating tumor cells is less (less than 5%), but has a high proportion of metastatic ability. Patients in whom circulating tumor cell adherent groups were found in peripheral blood tended to have shorter progression-free survival. Therefore, it is particularly important to study the adhesion groups of circulating tumor cells. [0004] However, in the...

Claims

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Application Information

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IPC IPC(8): C12M1/00
CPCC12M23/16
Inventor 彭年才闵帅超胡飞张朋周利
Owner XI AN JIAOTONG UNIV
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