Cell culture device for simulating cell mechanical microenvironment

Abstract

The invention discloses a cell culture device for simulating a cell mechanical microenvironment. The cell culture device comprises a gas driving device, a culture tank and a bulk acoustic wave generator, wherein a soft pneumatic cavity is arranged in the culture tank, a culture solution is inversely arranged on the surface of the soft pneumatic cavity, the soft pneumatic cavity is inflated and deflated through the gas driving device, the top of the soft pneumatic cavity is deformed, the deformation force of the soft pneumatic cavity is used as a loading method for macroscopic level mechanical stimulation of tissues, meanwhile, bulk acoustic waves are applied to cells in the culture solution through the bulk acoustic wave generator, the bulk acoustic waves are used as a loading method for microcosmic level mechanical stimulation of the cells, the bulk acoustic waves can act on all types of cells, accurate control over cells is achieved, meanwhile, sound wave control cannot cause damage to the cells, the sound flow control technology and the soft driving technology are combined, the cell culture device can give consideration to macroscopic and micromechanical stimulation on the cells, and simulation of the cell mechanical microenvironment in the in-vitro micro tissue culture process is achieved.

Description

technical field [0001] The invention belongs to the field of biotechnology, in particular to a cell culture device for simulating complex structures and mechanical microenvironments of tissue cells in vitro based on acoustic fluidic and soft-base drive technology. Background technique [0002] Organoids refer to the construction of microtissues or microorgans with three-dimensional multicellular complex structures and tissue functions in a microfluidic system, which are used to simulate the activities, mechanical environments and physiological responses of whole organs and multi-organ systems in vitro. The research on organoid chips has great application potential in the fields of life sciences, new drug development, personalized medicine, toxicity prediction and biodefense. In 2019, "Science" published a special issue on the theme of organoid chips, summarizing the application prospects of organoid chips and the problems faced by the current development, and pointing out th...

AI Technical Summary

Problems solved by technology

The existing micro- and macro-mechanical loading methods have disadvantages such as high price, complex equipment, interference to the micro-environment, and difficulty in achieving both macro- and micro-mechanical stimuli.

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