Dynamic load and recirculating perfusion bioreactor

Abstract

The invention belongs to the field of bone tissue engineering and cell mechanics, relating to a dynamic load and recirculating perfusion bioreactor, which comprises a main body, a sliding sleeve module and a three-dimensional culture cabin module, wherein the main body comprises a base, a side support plate and an upper support plate; the side support plate is provided with a concave sliding groove; the sliding sleeve module comprises a sliding sleeve, a piezoelectric ceramic, an up / down adjusting screw and a fastening screw; the sliding sleeve can upward and downward slide in the concave sliding groove stably; the piezoelectric ceramic passes through the sliding sleeve; the three-dimensional culture cabin module comprises a culture cabin main body of which the upper surface is provided with a through hole; the piezoelectric ceramic penetrates into the three-dimensional culture cabin through the through hole, the moving end of the piezoelectric ceramic is connected with a loading rod; and the culture cabin main body is also provided with a liquid inlet and a liquid outlet which are externally connected with a peristaltic pump. The invention has the characteristics of simple operation, high practicability, good aseptic sealing performance, high loading stress output accuracy, and timely and accurate data tracking records.

Description

technical field [0001] The invention belongs to the field of bone tissue engineering and cell biomechanics, and specifically relates to a method for dynamic culture of tissue engineering bone and research on the effect of dynamic compressive strain on mesenchymal stem cells, osteoblasts, osteoclasts, chondrocytes, etc. under three-dimensional culture conditions. Bioreactor for cell growth and differentiation effects. Background technique [0002] In recent years, studies on the stress-growth relationship of biological tissues have shown that life movements at all levels, from organs and tissues to cells and sub-cells, are carried out in a certain mechanical environment. The relationship between stress and growth is mainly manifested in two aspects at the cellular level: the biological behavior of a single cell, such as the influence of proliferation, morphology, and secretory function; Enhancement of function, interaction of osteocytes, osteoblasts, and osteoclasts in the p...

AI Technical Summary

Problems solved by technology

[0004] Regarding the stress-loaded bioreactors used for bone tissue and tissue engineering cultures, there are only a small number of documents and patent reports at home and abroad; the power system for dynamic stress or strain loading mainly adopts stepper motors, and there are many bioreactors. Insufficient, such as stress or strain loading and data tracking records are not accurate enough; it is impossible to perform real and effective long-term perfusion culture of tissue culture under sterile conditions at the same time; poor practical performance, low experimental efficiency, etc.

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