Cell mechanical property tester based on STM32

Abstract

The invention discloses a tester for cell mechanical properties based on STM32, which comprises a quartz crystal receiving device, a frequency generating part, a frequency collecting part and a microcomputer processing part. The quartz crystal receiving device includes a quartz crystal and a membrane electrode; the frequency generating part The part is composed of an oscillating circuit, a filter circuit and an AGC control circuit, the oscillating circuit, the filter circuit and the AGC control circuit are connected in sequence, the quartz crystal receiving device is connected with the oscillating circuit through an electric wire, and the frequency acquisition part includes a frequency / voltage The conversion circuit, the microcomputer processing part includes an STM32 chip and its external interface, and the frequency / voltage conversion circuit is connected with the STM32 chip through an electric wire. The invention adopts a non-invasive and label-free testing method to realize real-time, continuous and long-term testing, can be suitable for cell groups, and realizes the research and quantitative testing of cell functions.

Description

technical field [0001] The invention relates to a tester for cell mechanical properties, in particular to a tester for cell mechanical properties based on STM32. Background technique [0002] Quartz crystal microbalance (QCM) technology is a sensor measurement technology developed in the 1960s, which is widely used in molecular biology, microchemistry, environmental science and other detection fields. It uses the piezoelectric effect of the crystal to convert the tiny mass change on the quartz crystal electrode into a change in the vibration frequency that can be measured. Relevant studies have shown that at any time during the cell adhesion process, the surface stress or cell traction ΔS (unit: dyn / cm) generated or imposed on the crystal will cause the quartz crystal to produce a frequency shift Δf. This provides a relevant theoretical basis for testing the mechanical properties of cells with quartz crystals. [0003] The mechanical properties of cells are important param...

AI Technical Summary

Problems solved by technology

There are certain limitations in traditional animal cell mechanics methods: ① most of them are non-destructive and need to exert a certain force on the cells to realize the measurement, and the external force will lead to complex cell responses; ② most of them can only measure the mechanical properties of cells, Not suitable for cell populations; ③It is difficult to distinguish the mechanical properties of different cell structures; ④It cannot realize the non-destructive and long-term monitoring of cell mechanical parameters and realize the research and quantitative testing of cell functions

Images