9 results about "Cell deformation" patented technology

The invention discloses a microfluidic image flow cytometry identification system and a microfluidic image flow cytometry identification method based on multi-modal phase imaging and deep learning, relates to a microfluidic image flow cytometry detection technology, and belongs to the crossing field of microfluidics, optical imaging and artificial intelligence. The cell deformation chip is used for inducing a cell sample to generate controllable deformation through the cell deformation chip and discharging deformed cells; the optical imaging unit is used for scanning the deformed cells and collecting multi-modal phase images of the deformed cells; the data processing and analysis unit is used for preprocessing the multi-modal phase image and performing cell intelligent identification analysis on the preprocessed multi-modal phase image by using a deep learning feature extraction network, so that accurate identification and classification of cells are realized, and a leukocyte subpopulation identification model is constructed; and generating a cell imaging result and a cell mechanical parameter thermodynamic diagram. According to the invention, label-free identification of leukocyte subgroups is realized, and the problems of tedious operation and cell damage of traditional fluorescence labeling are solved.

The utility model discloses a plate structure, a battery device and a power utilization device, the plate structure comprises a first layer plate, a second layer plate and a third layer plate which are sequentially stacked along a first direction, and the second layer plate is a nylon particle foam plate. According to the plate structure, the nylon particle foam plate is clamped between the two layers of plates, the first layer of plate can disperse impact force, so that the foam plate is evenly stressed, the energy absorption characteristic is fully played, local failure is avoided, the internal foam structure of the nylon particle foam plate can effectively cope with impact, and the nylon particle foam plate is excellent in mechanical property, high in strength and high in toughness; when the foam board is impacted, energy is absorbed through foam hole deformation and nylon particle effect, the structure is kept stable, the third layer board provides supporting and restraining, excessive deformation of the foam board is prevented, and the integrity of the whole structure is guaranteed. The characteristics of the three layers of materials are complementary, an energy transfer path is optimized, the compression deformation capacity and the impact resistance capacity of the whole plate structure are greatly improved, structural safety and stability can be effectively guaranteed, the service life is prolonged, and the maintenance cost is reduced.

The application relates to the field of artificial intelligence medical technology and discloses an early esophageal cancer screening method and system based on artificial intelligence, which comprises the following steps: preparing an obtained esophageal epithelial cell sample of a subject into a single-layer cytological smear, generating an original digital pathology image through optical scanning; extracting independent cells therefrom, calculating local spatial moment parameters of the independent cells; determining the geometric principal axis direction and stretching degree of the independent cells based on the local spatial moment parameters; judging whether the stretching degree exceeds the length-width ratio benchmark constant of normal esophageal squamous epithelial cells, if yes, applying a local two-dimensional affine transformation to the independent cells along the geometric principal axis direction to offset the mechanical stretching deformation caused by sampling, and generating calibrated cell image data; inputting the calibrated cell image data into an artificial intelligence classification module, extracting cell pathological features based on a deep convolutional neural network, and outputting a screening diagnosis result. The application offsets cell deformation interference and improves screening accuracy.

This utility model discloses an external frame-type bulge monitoring power battery pack, including a cell module, a housing, a cover, a battery control system, and a cell bulge detection mechanism. The cell module is composed of multiple square cells arranged linearly and closely together, covered by an insulating plate and fixed by an external frame. The cell module is located inside the housing, and the cover covers the battery control system and closes to the housing to encapsulate the cell module inside the battery box. The cell bulge detection mechanism is set on the external frame and is used to detect the shape changes of the square cells caused by bulging in real time. This utility model achieves stable cell assembly and cell deformation monitoring through the cell bulge detection mechanism integrated on the external frame, improving the early safety warning capability of the battery pack.

The present application relates to a kind of real-time monitoring method for realizing signal molecule release in single cell deformation process by optical tweezers manipulation combined microelectrode sensing, comprising the following steps: obtaining ITO film microelectrode of electrically active area that can only accommodate one cell size;Preparation of working electrode that can specifically respond to signal molecule concentration change;With working electrode as bottom, build electrochemical chamber of three electrode system;With biomolecule modification microsphere carrier, and place it in electrochemical chamber;Two microsphere carriers are captured respectively by double optical trap optical tweezers and contacted with cell, and cell is stretched or extruded in working electrode area to deform, and the force spectrum information and deformation variable in cell deformation process are determined;Signal molecule concentration change released in cell deformation process is monitored in real time using electrochemical workstation.The present application can be used to study the relationship between the change of single cell release chemical information and cell mechanical information under mechanical force stimulation.

The invention belongs to the related technical field of micro-fluidic cell sorting, and discloses a micro-fluidic flow cell sorting system and method based on a cell deformation image, and the micro-fluidic flow cell sorting system comprises a micro-fluidic chip, the micro-fluidic chip comprises an inertia focusing structure, a cross-shaped cell stress deformation runner structure, a cross-shaped liquid drop generation structure, a liquid drop electrostatic separation structure and a liquid drop collection structure which are connected in sequence; the inertial focusing structure is used for focusing cells, so that the cells orderly enter the cross-shaped cell stress deformation runner structure; the cross-shaped cell stress deformation flow channel structure is used for enabling cells to deform through tensile shear force of fluid in a flow channel on the cells; the cross-shaped liquid drop generation structure is used for completely wrapping cells in liquid drops to form a completely closed liquid environment; the liquid drop electrostatic sorting structure is used for charging liquid drops as required, so that cell sorting is realized through a deflection electric field; and the liquid drop collecting structure is used for collecting the separated liquid drops. According to the invention, the integration level is improved.

This application relates to a support member, support device, and battery pack for the bottom of a battery pack, belonging to the technical field of battery packs. It absorbs impact energy and reduces cell deformation through the deformation of the support member; the support device consists of a sandwich structure of a bottom protective plate, the support member, and a liquid cooling plate, possessing high rigidity and cavity buffering energy absorption function; the liquid cooling plate is bonded to the battery module, and the casing is integrated with bolts for connection; additional reinforcing members further enhance local rigidity. This application, through structural optimization and layered collaborative design, absorbs impact energy through cross-sectional deformation, effectively reducing cell deformation and avoiding the energy absorption attenuation problem caused by plastic deformation in traditional rigid supports.

The application relates to a system and method for analyzing and detecting cell deformation characteristics in microdroplets, belonging to the field of microfluidic technology. The system comprises a microscopic imaging module, a data processing module, a capillary microchannel, a sample injection pump and a collection device; the microchannel is placed above a microscope object platform, a coaxial microchannel is adopted, a cell suspension is introduced into an inner tube, a continuous phase fluid is introduced into an outer tube, cells are wrapped in the suspension, the size of the droplet and the number of wrapped cells are controlled by changing the flow ratio, an outlet is connected with the collection device, and the collection device is used for collecting the detected cells and waste liquid; the capillary microchannel realizes mechanical extrusion of the cells in the microdroplets. The data processing module is connected with a microscope camera and is used for identifying the size change of the cells and analyzing the deformation characteristics according to characteristic parameters. The application generates cells wrapped by droplets in the microchannel and makes the cells pass through a narrow contraction area, analyzes the influence of the contraction area on the deformation of the cells in the microdroplets, and can realize efficient and accurate detection of the deformation of the cells in the microdroplets.

The invention relates to a method for characterising the deformability of cells or a portion of cells in a sell sample, the method comprising: culturing the cells on a microstructured plate having microgrooves with a predetermined width and depth for at least partially engaging the nuclei of the cells in one or more microgrooves, at least one portion of the surface of the microgrooves being a cell adhesion surface; measuring a fluorescence signal of the nuclei, which nuclei are pre-treated so as to emit fluorescence radiation; on the basis of the fluorescence signal measured for each nucleus, determining a fluorescence intensity profile and at least one morphological parameter of the nucleus; on the basis of the fluorescence intensity profile and the at least one morphological parameter, determining a deformation class of the nucleus in the depth of the microgrooves.