24 results about "Cell deformation" patented technology

The invention relates to the technical field of microfluidics, in particular to a high-throughput and high-efficiency cell extrusion microfluidic system and a particle introduction method. And a closed-loop control system integrating real-time cell deformation sensing, dynamic parameter decision and physical extrusion and alternating electric field cooperative application is constructed. The chip is provided with an inlet shunting device, a micro-channel and a channel structure of a microelectrode. The method comprises the steps of real-time deformation image acquisition and analysis and dynamic extrusion parameter decision and cooperative application. The invention provides a high-flux and high-efficiency cell extrusion microfluidic system and a particle introduction method, and aims to solve the problems that the drug loading efficiency and the survival rate are difficult to consider due to heterogeneity in the existing cell extrusion technology and a physical extrusion and electroporation coordinated regulation strategy is lacked, so that personalized precise treatment of cells is realized, and the cell quality is improved. The drug loading efficiency, the cell survival rate and the high throughput are obviously improved.

The invention discloses a microfluidic image flow cytometry identification system and a microfluidic image flow cytometry identification method based on multi-modal phase imaging and deep learning, relates to a microfluidic image flow cytometry detection technology, and belongs to the crossing field of microfluidics, optical imaging and artificial intelligence. The cell deformation chip is used for inducing a cell sample to generate controllable deformation through the cell deformation chip and discharging deformed cells; the optical imaging unit is used for scanning the deformed cells and collecting multi-modal phase images of the deformed cells; the data processing and analysis unit is used for preprocessing the multi-modal phase image and performing cell intelligent identification analysis on the preprocessed multi-modal phase image by using a deep learning feature extraction network, so that accurate identification and classification of cells are realized, and a leukocyte subpopulation identification model is constructed; and generating a cell imaging result and a cell mechanical parameter thermodynamic diagram. According to the invention, label-free identification of leukocyte subgroups is realized, and the problems of tedious operation and cell damage of traditional fluorescence labeling are solved.

This invention belongs to the field of biotechnology and discloses a dry film stabilizer, cytoplasmic antigen dry films, their preparation methods, and applications. The dry film stabilizer includes polyethylene glycol, trehalose, BSA, melatonin, and Tween-20. Cytoplasmic antigen dry films are prepared using this stabilizer and applied in CBA detection. This dry film stabilizer has good permeability, penetrating the cell membrane to enter the cell interior, providing protection both inside and outside the cell. It also possesses moisturizing and antioxidant properties, preventing cell deformation and shedding of the cell fixation layer, effectively maintaining the stability and reliability of intracellular antigen proteins. The prepared cytoplasmic antigen dry films can be stored long-term without damaging the antigenicity of cytoplasmic signal indicators. When detecting positive samples, strong and numerous green filamentous fluorescence signals are observed in the cytoplasm, with consistent green and red fluorescence intensities. Software synthesis reveals co-localization of green and red antigen fluorescence.

The present application relates to the technical field of microfluidic chip, and specifically discloses a microstructure spiral inertia microfluidic chip for cell deformation ability sorting and application thereof, the chip comprises a spiral flow channel, an inlet is arranged at the upstream end, a first outlet and a second outlet are arranged at the downstream end, and micro-pillars are arranged on the inner side wall of the spiral flow channel towards the curvature center at equal intervals. The present application strengthens the Dean secondary flow through the micro-pillar structure, and in a wide flow rate range, cells with different deformation abilities are respectively focused on the near inner wall and far inner wall regions of the flow channel based on the force balance difference, and high-throughput sorting without barriers is realized through the corresponding outlets, the sorting flux is more than 2 million cells / min, the migration and invasion ability of the soft cancer cell subpopulation obtained by sorting is 2-3 times that of hard cells, high-speed imaging can be matched to realize online quality control, and technical support is provided for tumor mechanical phenotype research.

The invention discloses a cell multi-modal analysis system and method based on a micro-fluidic chip, and the system comprises a data collection and processing module which is used for inputting a mixed cell sample, a first cell sample screened by a cell classification module and a second cell sample output by a cell three-dimensional reconstruction module into the micro-fluidic chip, respectively acquiring a first bright field image, a second bright field image and a third bright field image; the cell classification module is used for receiving the first bright field image, outputting a cell category probability value through a cell classification model, screening a first cell sample and feeding back the first cell sample to the data acquisition and processing module; the cell three-dimensional reconstruction module is used for receiving the second bright field image, outputting a second cell sample through a cell reconstruction model, and feeding back the second cell sample to the data acquisition and processing module; and the cell deformation analysis module is used for receiving the third bright field image and outputting deformation data through a cell deformation model. According to the invention, cell multi-modal analysis can be accurately and quickly carried out at low cost.

A mesoscopic unit cell analysis method for bending mechanical behaviors of a three-dimensional braided composite material relates to the field of mechanical property analysis, and comprises the following steps: obtaining mesoscopic characteristics and geometric parameters of a material, and establishing a mesoscopic unit cell finite element model with in-plane periodicity and thickness consistent with that of a sample; the method comprises the following steps: enabling a four-point bending sample to be equivalent to an in-plane periodically distributed thin plate structure, establishing association between macroscopic thin plate deformation and mesoscopic unit cell deformation based on a homogenization method, and deducing periodic boundary conditions; applying a boundary condition on the mesoscopic unit cell finite element model to simulate a mesoscopic mechanical response under a four-point bending load; and macroscopic bending performance parameters are obtained through microcosmic stress field homogenization treatment. According to the method, the in-plane periodic mesoscopic unit cell finite element model with the thickness consistent with that of the sample is established, the periodic boundary condition and the homogenization method are combined, macroscopic thin plate deformation and unit cell mesoscopic response are associated, and the applicability problem caused by non-periodicity of a traditional unit cell model in the thickness direction under the bending load is solved.

The utility model discloses a plate structure, a battery device and a power utilization device, the plate structure comprises a first layer plate, a second layer plate and a third layer plate which are sequentially stacked along a first direction, and the second layer plate is a nylon particle foam plate. According to the plate structure, the nylon particle foam plate is clamped between the two layers of plates, the first layer of plate can disperse impact force, so that the foam plate is evenly stressed, the energy absorption characteristic is fully played, local failure is avoided, the internal foam structure of the nylon particle foam plate can effectively cope with impact, and the nylon particle foam plate is excellent in mechanical property, high in strength and high in toughness; when the foam board is impacted, energy is absorbed through foam hole deformation and nylon particle effect, the structure is kept stable, the third layer board provides supporting and restraining, excessive deformation of the foam board is prevented, and the integrity of the whole structure is guaranteed. The characteristics of the three layers of materials are complementary, an energy transfer path is optimized, the compression deformation capacity and the impact resistance capacity of the whole plate structure are greatly improved, structural safety and stability can be effectively guaranteed, the service life is prolonged, and the maintenance cost is reduced.

The application belongs to the technical field of biology, and discloses a stabilizing protective agent, a cell antigen dry sheet, and a preparation method and application of the dry sheet. The stabilizing protective agent comprises 0.4% glycerol in volume fraction, 0.1% gelatin in mass volume fraction, 0.1 mM EDTA, 0.03% trehalose in mass volume fraction, and 100 muM resveratrol. The stabilizing protective agent is used to prepare the cell antigen dry sheet and is applied in CBA detection. The stabilizing protective agent has good permeability, penetrates into the cell interior through the cell membrane, provides protection inside and outside the cell, has moisturizing and antioxidant properties, can prevent cell deformation and cell fixation layer from falling off, well maintains the stability and reliability of antigen protein, and the prepared cell antigen dry sheet can be stored for a long time without damaging the antigenicity of the membrane signal index. When a positive sample is detected, obvious green fluorescent cells can be seen, and red fluorescent quenching does not occur, so that the co-localization of red fluorescence and green fluorescence can be observed.

The application relates to the field of artificial intelligence medical technology and discloses an early esophageal cancer screening method and system based on artificial intelligence, which comprises the following steps: preparing an obtained esophageal epithelial cell sample of a subject into a single-layer cytological smear, generating an original digital pathology image through optical scanning; extracting independent cells therefrom, calculating local spatial moment parameters of the independent cells; determining the geometric principal axis direction and stretching degree of the independent cells based on the local spatial moment parameters; judging whether the stretching degree exceeds the length-width ratio benchmark constant of normal esophageal squamous epithelial cells, if yes, applying a local two-dimensional affine transformation to the independent cells along the geometric principal axis direction to offset the mechanical stretching deformation caused by sampling, and generating calibrated cell image data; inputting the calibrated cell image data into an artificial intelligence classification module, extracting cell pathological features based on a deep convolutional neural network, and outputting a screening diagnosis result. The application offsets cell deformation interference and improves screening accuracy.

The application discloses a kind of bionic fish ladder microfluidic chip and integrated cell sorting and drug screening platform thereof.The fish ladder microfluidic chip adopts fish ladder-shaped main channel of tapering-dilating alternation, internally set micro barrier array with gap gradually decreasing, and vortex stop zone is formed in dilating section, overall imitates the ladder-ramp pool structure of "fish ladder" in fish migration channel.Based on cell deformation difference, it realizes label-free, mild, high-throughput sorting;After sorting, cell directly flows into downstream 3D culture chamber, can in situ carry out drug screening.The application integrates sorting, screening and culture in single chip, avoids damage and state change caused by cell transfer in traditional method.The "fish ladder microfluidic" concept defined in the application has clear bionic source and identifiable structural characteristics, and can be used as an iconic term in the technical field.Especially suitable for efficient screening of active ingredients in complex system of traditional Chinese medicine and tumor stem cell research.

The invention provides a quantitative loading method and device for a cell lamella, the quantitative loading device for the cell lamella comprises a pressure applying part, a pressure bearing part and a plurality of buffer springs, and the method comprises the following steps: injecting a cell culture medium containing cells into a cell bearing hole, and waiting for the cells to adhere to the wall; the pressure applying component is controlled to descend until the other end of the pressure applying sub-component is in contact with the cells; the position, making contact with the cells, of the other end of the pressure applying sub-component serves as an initial zero point, and displacement-time data and a microscope image sequence are synchronously collected; carrying out multiple times of compression loading on the cells, and collecting cell images after each time of compression loading; according to the collected cell image sequence and the preset displacement, speed and loading mode, analysis of the cell deformation quantitative detection operation system is carried out, and cell deformation data is obtained. At least the defects of easy channel blockage, fixed extrusion degree, low loading control precision, difficulty in real-time quantitative analysis of deformation and the like in the existing cell extrusion technology can be overcome.

This utility model discloses an external frame-type bulge monitoring power battery pack, including a cell module, a housing, a cover, a battery control system, and a cell bulge detection mechanism. The cell module is composed of multiple square cells arranged linearly and closely together, covered by an insulating plate and fixed by an external frame. The cell module is located inside the housing, and the cover covers the battery control system and closes to the housing to encapsulate the cell module inside the battery box. The cell bulge detection mechanism is set on the external frame and is used to detect the shape changes of the square cells caused by bulging in real time. This utility model achieves stable cell assembly and cell deformation monitoring through the cell bulge detection mechanism integrated on the external frame, improving the early safety warning capability of the battery pack.

The application provides a method, device and product for realizing four-dimensional scene interactive playing of a browser, which organizes dynamic display content into static base resources, dynamic base resources and Gaussian cell deformation attribute label maps corresponding to each video frame, and combines the label maps into a Gaussian rendering object after loading at the browser end; during playing, a time axis determines a current target frame, only the dynamic Gaussian cells are written back according to frame attributes, and the static Gaussian cells remain constant and are not repeatedly updated; a user can also rotate, zoom, translate and switch a view angle through a virtual camera. Thus, the dynamic process can be continuously played like a video, and the view angle can be freely viewed like a three-dimensional model, and the complete three-dimensional scene is avoided to be loaded frame by frame, the loading, memory and rendering pressure of the browser end is reduced, and the application is suitable for dynamic interactive display scenes such as commodity display, handicraft process playback, teaching demonstration and live playback.

The present application relates to a kind of real-time monitoring method for realizing signal molecule release in single cell deformation process by optical tweezers manipulation combined microelectrode sensing, comprising the following steps: obtaining ITO film microelectrode of electrically active area that can only accommodate one cell size;Preparation of working electrode that can specifically respond to signal molecule concentration change;With working electrode as bottom, build electrochemical chamber of three electrode system;With biomolecule modification microsphere carrier, and place it in electrochemical chamber;Two microsphere carriers are captured respectively by double optical trap optical tweezers and contacted with cell, and cell is stretched or extruded in working electrode area to deform, and the force spectrum information and deformation variable in cell deformation process are determined;Signal molecule concentration change released in cell deformation process is monitored in real time using electrochemical workstation.The present application can be used to study the relationship between the change of single cell release chemical information and cell mechanical information under mechanical force stimulation.

The invention belongs to the related technical field of micro-fluidic cell sorting, and discloses a micro-fluidic flow cell sorting system and method based on a cell deformation image, and the micro-fluidic flow cell sorting system comprises a micro-fluidic chip, the micro-fluidic chip comprises an inertia focusing structure, a cross-shaped cell stress deformation runner structure, a cross-shaped liquid drop generation structure, a liquid drop electrostatic separation structure and a liquid drop collection structure which are connected in sequence; the inertial focusing structure is used for focusing cells, so that the cells orderly enter the cross-shaped cell stress deformation runner structure; the cross-shaped cell stress deformation flow channel structure is used for enabling cells to deform through tensile shear force of fluid in a flow channel on the cells; the cross-shaped liquid drop generation structure is used for completely wrapping cells in liquid drops to form a completely closed liquid environment; the liquid drop electrostatic sorting structure is used for charging liquid drops as required, so that cell sorting is realized through a deflection electric field; and the liquid drop collecting structure is used for collecting the separated liquid drops. According to the invention, the integration level is improved.

The present application provides a kind of cell precision dialing method based on moment balance, belongs to the field of micro-operation technology at cell level, comprising the following steps: S1: by analyzing the moment balance condition of cell critical holding state, determine the minimum holding force required for fixed cell not to separate from holding needle;S2: by force analysis to the cell in dialing process, determine the minimum dialing force expression required for cell moment balance;S3: friction push experiment determines the friction coefficient between holding needle-cell and dialing needle-cell;S4: dialing based on moment balance, reduce the damage to cell and improve dialing precision.The present application uses minimum holding force to fix cell, applies minimum dialing force to target cell by force position control of dialing needle, so that cell is in equilibrium state, reduces the average cell deformation in dialing process to less than 15% of artificial operation, reduces cell dialing error to within 1°.

This application relates to a support member, support device, and battery pack for the bottom of a battery pack, belonging to the technical field of battery packs. It absorbs impact energy and reduces cell deformation through the deformation of the support member; the support device consists of a sandwich structure of a bottom protective plate, the support member, and a liquid cooling plate, possessing high rigidity and cavity buffering energy absorption function; the liquid cooling plate is bonded to the battery module, and the casing is integrated with bolts for connection; additional reinforcing members further enhance local rigidity. This application, through structural optimization and layered collaborative design, absorbs impact energy through cross-sectional deformation, effectively reducing cell deformation and avoiding the energy absorption attenuation problem caused by plastic deformation in traditional rigid supports.

The application relates to a system and method for analyzing and detecting cell deformation characteristics in microdroplets, belonging to the field of microfluidic technology. The system comprises a microscopic imaging module, a data processing module, a capillary microchannel, a sample injection pump and a collection device; the microchannel is placed above a microscope object platform, a coaxial microchannel is adopted, a cell suspension is introduced into an inner tube, a continuous phase fluid is introduced into an outer tube, cells are wrapped in the suspension, the size of the droplet and the number of wrapped cells are controlled by changing the flow ratio, an outlet is connected with the collection device, and the collection device is used for collecting the detected cells and waste liquid; the capillary microchannel realizes mechanical extrusion of the cells in the microdroplets. The data processing module is connected with a microscope camera and is used for identifying the size change of the cells and analyzing the deformation characteristics according to characteristic parameters. The application generates cells wrapped by droplets in the microchannel and makes the cells pass through a narrow contraction area, analyzes the influence of the contraction area on the deformation of the cells in the microdroplets, and can realize efficient and accurate detection of the deformation of the cells in the microdroplets.

The utility model relates to a kind of lithium battery cell thickness measuring fixture, to solve current manual measuring tool, error is larger, and traditional measuring tool is usually needed to hold down cell in the measuring process, this not only leads to the cell is more troublesome to take and place, also possibly due to improper clamping force leads to the technical problem of cell deformation, including base and vertical board, the surface of vertical board is fixedly connected with two lifting frames, and the upper and lower inner walls of lifting frame are fixedly connected with slide bar, two slide bars are vertically through the front and rear surfaces of lifting plate and are slidably connected with lifting plate, the bottom of lifting plate is fixedly connected with abutting head, the utility model is driven abutting head to move by setting lifting plate between lifting frame, to let abutting head freely abut cell when measuring, avoid the problem that cell deformation can be caused by the clamping of traditional measuring appliance to cell, it is convenient and fast to take and place cell, only need to lift abutting head, without repeatedly releasing clamping, convenience is improved.

In a method of investigating cell deformations, a sample fluid including cells suspended in a suspending medium is provided. A flow of the sample fluid through a focusing microchannel is established. The suspending medium is a non-Newtonian fluid having viscoelastic properties such that cells that enter the focusing microchannel are focused towards a center of the focusing microchannel due to the viscoelastic properties of the suspending medium, causing the cells to exit the focusing microchannel in single file. Subsequently, the sample fluid that has exited the focusing microchannel is caused to flow through a deformation microchannel arranged downstream of the focusing microchannel to cause a deformation of cells that have exited the focusing microchannel and have entered the deformation microchannel, the deformation being caused by a flow pattern created by interaction of the fluid flow with the deformation microchannel.

The invention relates to a method for characterising the deformability of cells or a portion of cells in a sell sample, the method comprising: culturing the cells on a microstructured plate having microgrooves with a predetermined width and depth for at least partially engaging the nuclei of the cells in one or more microgrooves, at least one portion of the surface of the microgrooves being a cell adhesion surface; measuring a fluorescence signal of the nuclei, which nuclei are pre-treated so as to emit fluorescence radiation; on the basis of the fluorescence signal measured for each nucleus, determining a fluorescence intensity profile and at least one morphological parameter of the nucleus; on the basis of the fluorescence intensity profile and the at least one morphological parameter, determining a deformation class of the nucleus in the depth of the microgrooves.

The invention belongs to the technical field of biology, and discloses a dry slice stabilizer, a cytoplasm antigen dry slice and a preparation method and application of the cytoplasm antigen dry slice, the dry slice stabilizer comprises polyethylene glycol, trehalose, BSA, melatonin and Tween-20, the dry slice stabilizer is adopted to prepare the cytoplasm antigen dry slice, and the cytoplasm antigen dry slice is applied to CBA detection. The dry sheet stabilizer has good permeability, can penetrate through cell membranes to enter cells, provides a protection effect inside and outside the cells, has moisture retention and oxidation resistance, can prevent the cells from deforming and a cell fixing layer from falling off, well maintains the stability and reliability of antigen protein in cytoplasm, and can be used for preparing the dry sheet. The prepared cytoplasm antigen dry sheet can be stored for a long time, and the antigenicity of cytoplasm signal indexes is not damaged. When a positive sample is detected, green filamentous fluorescence signals in cytoplasm are strong and large in number, green fluorescence and red fluorescence are consistent in intensity, and co-localization of green fluorescence and red antigen fluorescence can be observed through software synthesis.

The present invention relates to the technical field of cell-level microscopic operations. Provided is a method for precise cell manipulation based on torque balance, the method comprising the following steps: S1, analyzing a torque balance condition at a critical cell holding state, and determining the minimum holding force required to keep a cell fixed without detaching from a holding pipette; S2, performing a stress analysis on the cell that is being manipulated, and determining the expression for the minimum manipulation force required to balance the torque acting on the cell; S3, determining the friction coefficients between the holding pipette and the cell, and between a manipulation needle and the cell; and S4, carrying out manipulation on the basis of torque balance so as to reduce damage to the cell and to improve the manipulation precision. The cell is fixed by using the minimum holding force, and the minimum manipulation force is applied to a target cell by the force site control of the manipulation needle, such that the cell is in a torque-balanced state. The average cell deformation during the manipulation is reduced to be 15% or less of that observed during manual operations, and the error in cell manipulation is reduced to be within 1°.

The invention generally relates to the field of intracellular delivery In an aspect of the invention, there is provided a method for delivering a cargo into a cells, the method comprising providing a mixture comprising of the cell and the cargo suspended in a viscoelastic fluid, and deforming the cells in the mixture, the deformation creates transient pores in the cell's membrane allowing delivery of the cargo into the cells.