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Cell culture room, adjustable cell mechanical stimulation culture device and manufacturing method thereof

A technology of cell culture and production method, which is applied in the field of adjustable cell mechanical stimulation culture device and cell culture room, which can solve the problems of difficulty in feeling multi-directional mechanical stimulation and single mechanical loading mode, so as to meet the needs of small-scale cell culture and facilitate different The effect of experimental requirements

Active Publication Date: 2022-07-15
SICHUAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0010] This application provides a cell culture room, an adjustable cell mechanical stimulation culture device and its manufacturing method, which firstly solves the problem that the existing fixed culture room is difficult to experience multi-directional mechanical stimulation; secondly, it solves the first problem of the topological structure of cell culture , and finally solve the problem of a single mechanical loading mode

Method used

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  • Cell culture room, adjustable cell mechanical stimulation culture device and manufacturing method thereof
  • Cell culture room, adjustable cell mechanical stimulation culture device and manufacturing method thereof
  • Cell culture room, adjustable cell mechanical stimulation culture device and manufacturing method thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0065] like figure 1 , figure 2 As shown, the cell culture chamber disclosed in this embodiment includes an elastic box 1 made of elastic material, and the elastic box 1 has at least one culture compartment 10 therein.

[0066] The number of culture compartments 10 in the elastic box 1 and the shape of the elastic box 1 are set as required. like figure 1 As shown, in some embodiments, the flexible box 1 is a rectangular box consisting of four side walls 11 , one bottom plate and only one culture compartment 10 . In other embodiments, a cross-shaped partition is arranged in the rectangular elastic box 1 to separate the inside of the elastic box 1 into four culture compartments 10, such as figure 2 shown.

[0067] In order to facilitate the mechanical stimulation of the cells, the elastic box 1 is provided with a connecting structure for connecting with the mechanical loading device. The connecting structure can be a concave structure or a convex structure, or a concave s...

Embodiment 2

[0075] This embodiment discloses a method for fabricating a cell culture chamber, specifically: the elastic box 1 is formed by perfusion with PDMS.

[0076] The internal stress of the matrix hardness can be adjusted as required. By adjusting the ratio of the crosslinking agent to the base fluid, the culture substrate units with different hardness can be realized, which basically meet the requirements for the hardness of the cell culture substrate in different experimental designs and application scenarios.

[0077] PDMS formulated in some embodiments includes 1 part crosslinker, 10 parts base fluid.

[0078] PDMS formulated in some embodiments includes 1 part crosslinker, 20 parts base fluid.

[0079] PDMS formulated in some embodiments includes 1 part crosslinker, 30 parts base fluid.

[0080] PDMS formulated in some embodiments includes 1 part crosslinker, 40 parts base fluid.

[0081] PDMS formulated in some embodiments includes 1 part crosslinker, 50 parts base fluid. ...

Embodiment 3

[0090] This embodiment provides an adjustable cell mechanical stimulation culture device, such as Figure 10 , Figure 11 As shown, it includes a left loading device, a right loading device, a longitudinal loading device, and a cell culture chamber. The left loading device, the right loading device, and the longitudinal loading device are used to apply force to the cell culture chamber from three different directions, respectively. The left loading device, the right loading device and the longitudinal loading device are respectively connected with the elastic box 1 through the connection structure.

[0091]The connection positions of the left loading device, the right loading device, the longitudinal loading device and the elastic box 1 are respectively located in three directions of the elastic box 1 . In particular, the force application direction of the left loading device and the right loading device is the transverse direction, and the force application direction of the...

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Abstract

The invention relates to a cell culture room, an adjustable cell mechanical stimulation culture device and a manufacturing method thereof, the cell culture room comprises an elastic box made of an elastic material, and at least one culture compartment is arranged in the elastic box. PDMS is poured into the mold, the cell culture room is obtained after molding, and the PDMS comprises 1 part of a cross-linking agent and 10-50 parts of a substrate solution. The adjustable cell mechanical stimulation culture device comprises at least one mechanical loading unit and the cell culture chamber, each mechanical loading unit comprises a longitudinal fixing sheet, a left loading plate fixing sheet, a right loading plate fixing sheet and a longitudinal loading plate fixing sheet; and the left loading plate fixing sheet, the right loading plate fixing sheet and the longitudinal loading plate fixing sheet are connected with driving parts for driving the fixing sheets to move linearly. According to the invention, multi-time-sequence mechanical loading observation can be realized in the same batch, so that the controllability and authenticity of an in-vitro test are improved to a great extent; and the purposes of controllability of a single mechanical loading module, multi-parameter adjustability, flexible assembly of internal and external stress and the like can be achieved.

Description

technical field [0001] The invention relates to the technical field of cell culture, in particular to a cell culture chamber, an adjustable cell mechanical stimulation culture device and a manufacturing method thereof. Background technique [0002] The microenvironment of cells in the living body is very complex and changeable. As a dynamic system, it constantly interacts with the external environment and is continuously stimulated by external mechanical and biological signals. For example, vascular endothelial cells are continuously sheared by blood flow, muscle cells and bone tissue cells are subjected to mechanical stretch stress, periodontal ligament cells are stimulated by chewing force, etc., all of which are regulated by exogenous mechanical stress. biological process. At the same time, different cell matrix stiffness, topological structure of microenvironment, etc. act as endogenous stress to regulate cell morphology and function. It can be seen that both exogenous...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12M3/00C12M1/36C12M1/00C12N5/00
CPCC12M23/34C12M25/06C12M25/14C12M29/10C12M41/48C12N5/0068Y02P60/21
Inventor 蔡靖仪经典温记明赵志河熊瑞平朱冠印尧可
Owner SICHUAN UNIV
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