Device and method for measuring erythrocyte mechanical properties

Abstract

The invention discloses an erythrocyte mechanical property measuring device and method. The erythrocyte mechanical property measuring device comprises an injection pump, a power-adjustable signal generator, a surface acoustic wave driving array module, a temperature control module, a microscope, a CCD camera and a computer. The surface acoustic wave driving array module comprises a piezoelectric substrate, a first interdigital transducer, a second interdigital transducer, a third interdigital transducer, a fourth interdigital transducer, a fifth interdigital transducer, a sixth interdigital transducer and a micro-channel bonded on the piezoelectric substrate, wherein the first interdigital transducer, the second interdigital transducer, the third interdigital transducer, the fourth interdigital transducer, the fifth interdigital transducer and the sixth interdigital transducer are deposited on the piezoelectric substrate. The piezoelectric substrate is tightly attached to the upper surface of the temperature control module. Erythrocytes flowing into the micro-channel are deformed under the drive of acoustic surface waves generated by the interdigital transducer, and mechanical properties are calculated by using a mathematical model by analyzing erythrocyte deformation parameters shot by the CCD camera. The device and the method can be used for measuring the mechanical propertyof a single red blood cell with the particle size of less than 10 microns and simultaneously reflecting the elastic mechanical property and the tension property.

Description

technical field [0001] The invention relates to the fields of signal detection and physical parameter measurement, in particular to a device and method for measuring mechanical properties of red blood cells. Background technique [0002] Red blood cells are primarily composed of phospholipid bilayers, membrane contractile proteins, and a cytoskeletal network. The lipid bilayer is highly resistant to changes in local surface area, but the elasticity of the cytoskeleton enables RBCs to withstand large tensile deformations while maintaining the structural integrity of the membrane. RBC deformation can affect the cells' ability to release various molecules and regulate their concentrations in the blood. In particular, when RBCs are strongly deformed by the surrounding fluid, they release adenosine triphosphate (ATP) and adenosine diphosphate (ADP). ATP is the energy source for intracellular functions, and extracellular ATP as a signaling molecule plays an important role in a va...

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Problems solved by technology

It is difficult for the above-mentioned methods to measure the surface tension of droplets below the scale of 10 microns, and the size of red blood cells is much smaller than that of ordinary droplets, generally around 7 microns

In addition, the erythrocyte membrane is very fragile, and the above-mentioned methods are easy to cause membrane rupture, so it is difficult to measure the tension of the erythrocyte membrane, and it cannot reflect the elastic mechanical properties at the same time

Peng Hange (Peng Hange, Zhou Tie'an, Tan Chengfang, etc. QCM monitoring the viscoelastic response of neonatal rat primary cardiomyocytes under the action of different concentrations of inotropic drugs [J]. Acta Laser Biology, 2019, 28(3): 239-244.) proposed to use Quartz crystal microbalance (QCM) can monitor mechanical indicators such as cell viscoelastic parameters, but cannot detect single cells, and can only measure viscoelasticity, but cannot reflect cell membrane tension

Yuan Yumo (Yuan Weimo, Xue Chundong, Liu Bo, etc. A microfluidic chip for high-throughput measurement of single-cell elastic modulus [J]. Beijing Biomedical Engineering, 2019, 38(5): 450-456.) Although microfluidic chips can be used The fluidic chip can measure the elastic modulus of a single cell, but it cannot reflect the tension characteristics of the cell membrane

Yang Qian (Yang Qian, Sun Runguang, Zhang Jing et al. Combining AFM to detect the biomechanical properties of Salmonella paratyphi B-infected erythrocyte membrane[J]. Chinese Science: Life Science, 2014,44(2):197-207.) Measuring mechanical properties of red blood cell membranes using atomic force microscopy and diffraction microscopy, but atomic force microscopy is too expensive

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