Micro-fluidic chip-level extracorporeal circulation system for vascular endothelial cell mechanics and biology research

Abstract

The invention discloses a micro-fluidic chip-level extracorporeal circulation system for vascular endothelial cell mechanics biological research, and belongs to the technical field of cell mechanics biological experiment devices. The system comprises three parts: 1) the micro-fluidic chip is composed of a sandwich structure cell culture cavity and a multi-element fluid mechanics loop for simulating hemodynamic characteristics; 2, the fluid loading device is combined with a feedback control system, and hemodynamic signals such as blood pressure, wall shear stress and circumferential stretchingstrain borne by different target artery endothelium can be generated in the cell culture cavity; 3) the signal acquisition and processing system can be used for observing cell mechanics biological response in real time and feeding back detection data to the control system so as to further adjust the fluid loading device. The system accurately simulates a real target artery vascular endothelial extracellular hemodynamic microenvironment, and provides a miniaturized, objective, standardized and quantified experimental platform for researching a quantitative relationship between hemodynamic signals and a vascular endothelial cell mechanics biological mechanism.

Description

technical field [0001] The invention belongs to the technical field of cell mechanics biology experiment device for health and rehabilitation engineering, and is designed based on hemodynamic principle, microfluidic chip and electronic information technology, and consists of microfluidic chip, peripheral fluid loading device, signal acquisition and processing A microfluidic chip-level in vitro simulated circulatory system composed of a feedback control system for studying the quantitative relationship between hemodynamic signals caused by different physiological and pathological states and mechanical therapy and the mechanobiological effects of vascular endothelial cells. Background technique [0002] The arterial wall is composed of three layers of tissue: intima, media and adventitia. Among them, the intima refers to the single-layer structure of endothelial cells located in the innermost layer of the artery wall, also known as the endothelium. As a barrier between blood ...

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Problems solved by technology

Although the existing microfluidic systems can realize simple hemodynamic signals in the circulatory system, there is still a lack of a set of accurate simulations of the hemodynamic microenvironment of the target artery endothelium under different physiological and pathological conditions and mechanotherapy intervention conditions. Systematic theory and method; at the same time, the hydrodynamic circuit used to characterize the afterload hemodynamic characteristics of the target artery downstream of the cell culture chamber uses off-chip large-scale concentrated parameter components, and there is still room for improvement in terms of integration and consumables ; In addition, most of the dynamic loading of hemodynamic signals in the simulated circulatory system adopts open-loop control technology, which fails to achieve closed-loop control of pressure or flow signals; moreover, the detection of endothelial cell function in the cell culture chamber is mostly done offline Sampling for analysis fails to achieve online and real-time quantitative monitoring of endothelial cell mechanobiological response

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