A microfluidic chip-scale extracorporeal circulation system for mechanobiology research of vascular endothelial cells

Abstract

The invention relates to a microfluidic chip-level extracorporeal circulation system for the mechanobiology research of vascular endothelial cells, which belongs to the technical field of cell mechanobiology experimental devices. The system includes three parts: 1) The microfluidic chip is composed of a "sandwich" structure cell culture chamber and a multi-element hydrodynamic circuit simulating hemodynamic characteristics. 2) The fluid loading device combined with the feedback control system can generate hemodynamic signals such as blood pressure, wall shear stress and circumferential stretch strain borne by different target arterial endothelium in the cell culture chamber. 3) The signal acquisition and processing system can observe the cell mechanobiological response in real time and feed back the detection data to the control system to further adjust the fluid loading device. The system accurately simulates the real target arterial endothelial extrahemodynamic microenvironment, and provides miniaturization, objectification, standardization and quantification for studying the quantitative relationship between hemodynamic signals and the mechanobiological mechanism of vascular endothelial cells experimental platform.

Description

technical field [0001] The invention belongs to the technical field of cell mechanics biology experiment device for health and rehabilitation engineering, and is designed based on hemodynamic principle, microfluidic chip and electronic information technology, and consists of microfluidic chip, peripheral fluid loading device, signal acquisition and processing A microfluidic chip-level in vitro simulated circulatory system composed of a feedback control system for studying the quantitative relationship between hemodynamic signals caused by different physiological and pathological states and mechanical therapy and the mechanobiological effects of vascular endothelial cells. Background technique [0002] The arterial wall is composed of three layers of tissue: intima, media and adventitia. Among them, the intima refers to the single-layer structure of endothelial cells located in the innermost layer of the artery wall, also known as the endothelium. As a barrier between blood ...

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Problems solved by technology

Although the existing microfluidic systems can realize simple hemodynamic signals in the circulatory system, there is still a lack of a set of accurate simulations of the hemodynamic microenvironment of the target artery endothelium under different physiological and pathological conditions and mechanotherapy intervention conditions. Systematic theory and method; at the same time, the hydrodynamic circuit used to characterize the afterload hemodynamic characteristics of the target artery downstream of the cell culture chamber uses off-chip large-scale concentrated parameter components, and there is still room for improvement in terms of integration and consumables ; In addition, most of the dynamic loading of hemodynamic signals in the simulated circulatory system adopts open-loop control technology, which fails to achieve closed-loop control of pressure or flow signals; moreover, the detection of endothelial cell function in the cell culture chamber is mostly done offline Sampling for analysis fails to achieve online and real-time quantitative monitoring of endothelial cell mechanobiological response

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