The invention belongs to the scientific field of materials, particularly relates to a preparation method for a multielement dissymmetrical microsphere and a heterogeneous microsphere shell. The method comprises the following steps: a single-layered non-close-packed silicon dioxide colloidal crystal is prepared through an improved micro-contact printing technology, and the dissymmetrical microsphere and the heterogeneous microsphere shell are prepared through the combination with an angle-controllable non-close-packed silicon dioxide colloidal crystal deposition and etching technology. During the whole process, the operation is simple and convenient, the consumption is low, the process is clean, the controllability is high, and high stability of the dissymmetrical microsphere and the microsphere shell are achieved. Various dissymmetrical microspheres compounded by various kinds of materials are simply prepared through controlling the period, the deposition angle and the deposition frequency of the non-close-packed colloidal crystal and the kind of the deposition materials, and the corresponding heterogeneous microsphere shell can be obtained through chemically etching the silicon dioxide microsphere. The dissymmetrical microsphere and the heterogeneous microsphere shell, which are prepared through the invention, have important significance in scientific researches or practical applications.