The invention discloses a preparation method of a large-area surface enhancement
raman scattering substrate. The preparation method comprises the following steps: at first, the surface of a hard
solid material is irradiated by
femtosecond laser to prepare a large-scale hard microstructural mother set; then,
polydimethylsiloxane (PDMS) is poured on the hard microstructural mother set by the
nanoimprint lithography technology, and the structure on the hard microstructural mother set is copied to prepare a PDMS framework; and finally, a
gold film is plated on the PDMS framework to prepare the PDMS surface enhancement
raman scattering substrate. The experiment results show that the preparation method has the advantages that the enhancement factors are high, enhancement factors in different positions of the substrate have good uniformity, the large-area surface enhancement
raman scattering substrate can be prepared, the mother set can be repeatedly imprinted, the production cost is reduced, and in addition, god is taken as raman enhancement
metal, so that the defect that silver is oxidized very easily is avoided.