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Cell patterning structure, preparation method and application in cell proliferation inhibition

A cell pattern, cell technology, applied in biochemical equipment and methods, immobilized on/in organic carriers, bioreactors/fermenters for specific purposes, etc., to achieve easy access to equipment, convenient operation methods, and simple equipment Effect

Inactive Publication Date: 2014-12-03
JILIN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the study of cells in the pores of this honeycomb film is limited to some selective adsorption experiments (LEON A S D, HERNANDEZ J R, CORTAJARENA A L, Biomaterials 2013, 34, 1453-1460)

Method used

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  • Cell patterning structure, preparation method and application in cell proliferation inhibition
  • Cell patterning structure, preparation method and application in cell proliferation inhibition
  • Cell patterning structure, preparation method and application in cell proliferation inhibition

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Dissolve 30mg PS (purchased from Sigma-Aldrich, molecular weight: 350,000) and 0.5mg DDAB in 5mL dichloromethane to prepare an organic polymer solution, mix 50μL deionized water with 1mL polymer organic solution, and sonicate for 10min Obtain a microemulsion, filter this microemulsion with a 220nm filter head, and drop 20 μL of the obtained filtrate on a smooth glass that has been washed with deionized water, ethanol, and deionized water in sequence at a humidity of 30% and a temperature of 25°C. on the base. After the dichloromethane and deionized water are completely volatilized, an ordered porous film with a pore diameter of about 5.5 μm and a pore depth of about 2 μm is obtained on the surface of the polymer membrane of the substrate.

[0022] attached figure 1 is the scanning electron micrograph of the obtained ordered porous film and the Gaussian distribution diagram of the hole size. figure 1 a is the scanning electron micrograph of the prepared film, which sho...

Embodiment 2

[0029] As shown in Example 1, other conditions remain unchanged, the mass of DDAB is changed to 1.0 mg, the film-making temperature is changed to 30 ° C, and the humidity is changed to 40%, to obtain an organic film with a pore diameter of about 4 μm and a pore depth of about 1.8 μm. Sequenced porous films.

[0030] Then, according to the steps of Example 1, the assembly of polyelectrolyte PSS and PEI in the well, the selective distribution of cells in the well and the inhibition of cell proliferation based on the selective distribution were realized respectively.

Embodiment 3

[0032] As shown in Example 1, other conditions remain unchanged, and the negatively charged polyelectrolyte PSS is replaced by PAA to realize the assembly of PAA and PEI in the well, and then according to the steps in Example 1 to realize the selective distribution and distribution of cells in the well. Inhibition of cell proliferation based on selective distribution.

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Abstract

The invention discloses a cell patterning structure based on an ordered polymer film, a preparation method and an application in cell proliferation inhibition, belonging to the technical field of cell positioning and assembling. The method comprises the following steps: preparing an ordered porous film the inner wall of which is loaded with a positive charge surfactant, selectively assembling polyelectrolyte with charges which is opposite to that of cells on the inner walls of pores of the ordered porous film, selectively adsorbing inside the pores through the cells, and the like. The cell patterning structure disclosed by the invention is simple and easily available, convenient to operate, gentle in condition, short in period and capable of achieving selective distribution of cells in the ordered honeycomb-shaped film pores, and moreover through selection of cells of appropriate sizes, a single cell array in which each pore only has one cell can be prepared. The single cell array has significant meanings in the fields such as biosensors, tissue engineering, single cell carrying and behavior operation. The structure has significant application values in aspects such as cell transferring and transportation, cell proliferation inhibition and treatment on cancer and other diseases.

Description

technical field [0001] The invention belongs to the technical field of cell positioning assembly, patterned structure preparation and cell activities in a limited space, and specifically relates to a cell patterned structure based on an ordered polymer film, a preparation method and the effect of the structure on cell proliferation inhibition application. Background technique [0002] Cell-patterned structures have aroused great interest in the fields of cell biology, drug delivery, biomembrane, biosensor, and cell behavior research because of the precise control of the location of cells on the patterned substrate (FUKUDA J, KHADEMHOSSEINI A, YEH J, ENG G, CHENG J J, FAROKHZAD OC, LANGER R, Biomaterials 2006, 27, 1479-1486). Correspondingly, many methods such as microcontact printing (LAWRENCE N J, WEELS-KINGSBURY J M, IHRIG M M, FANGMAN T E, NAMAVAR F, CHEUNG C L, Langmuir 2012, 28, 4301-4308), photolithography (ROZHOK S, FAN Z F, NYAMJAV D, LIU C, MIRKIN C A, HOLZ R C, L...

Claims

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Application Information

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IPC IPC(8): C12M1/00C08J9/42C08J9/28C08L25/06C08L33/12
CPCC12N11/08
Inventor 马英一梁静吴立新
Owner JILIN UNIV
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