Arbitrarily shaped, deep sub-wavelength acoustic manipulation for microparticle and cell patterning

Abstract

The present invention relates to a near-field acoustic platform capable of synthesizing high resolution, arbitrarily shaped energy potential wells. A thin and viscoelastic membrane is utilized to modulate acoustic wavefront on a deep, sub wavelength scale by suppressing the structural vibration selectively on the platform. This new acoustic wavefront modulation mechanism is powerful for manufacturing complex biologic products.

Description

[0001] Cross References to Related Applications [0002] This application claims priority to U.S. Provisional Patent Application No. 62 / 837,768, filed April 24, 2019, the contents of which are hereby incorporated by reference in their entirety. [0003] Statement Regarding Federally Sponsored Research or Development [0004] This invention was made with government support under Grant No. 1711507 from the National Science Foundation. The Government has certain rights in this invention. Background technique [0005] Methods for manipulating biological objects on the micrometer to centimeter scale are fundamental to many biomedical applications, including the study of cell-cell interactions (Nilsson J et al., Analytica chimica acta, 649(2), 141-157; Sun J et al., Biomaterials, 35(10), 3273-3280), single cell analysis (Wood DK et al., Proceedings of the National Academy of Sciences, 107(22), 10008-10013; Collins DJ et al., Lab ona Chip, 15 (17), 3439-3459), drug development (Ka...

AI Technical Summary

Problems solved by technology

Other methods based on electrokinetics, such as dielectrophoresis and electroosmosis, are simple to implement but are challenged by buffer incompatibility and electrical interference, which can damage manipulated samples

3D printing (Chia HN et al., Journal of biological engineering, 9(1), 4; Panwar A et al., Molecules, 21(6), 685) provides another means to form complex patterned contours, but has not yet Enables precise control over what it prints, thus limiting resolution

However, due to the nature of standing waves, SAWs face similar problems with limited patterned profiles that are often symmetrical

Furthermore, the rapid decay of SAW due to energy transfer to the fluid makes large-area patterning difficult; typical SAW devices cannot operate in areas larger than 1 mm × 1 mm (Collins DJ et al., Nature communications, 6, 8686)

Images