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Device and method for cell patterning culture based on 3D printing technique

A 3D printing and cell culture technology, applied in the field of 3D printing technology and cell culture, to achieve the effect of low technical requirements, avoiding waste of time and money, reducing the requirements of clean space and complex equipment

Pending Publication Date: 2017-08-18
UNIV OF SCI & TECH BEIJING
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] The invention of using 3D printing to manufacture micron-scale precision devices and apply it to cell biology, especially cell micropatterning, has not been reported yet

Method used

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  • Device and method for cell patterning culture based on 3D printing technique
  • Device and method for cell patterning culture based on 3D printing technique
  • Device and method for cell patterning culture based on 3D printing technique

Examples

Experimental program
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Effect test

Embodiment 1

[0033] Such as figure 1 As shown, a multi-cell co-culture device is provided, including: a 3D printed device and a cell culture dish or a culture plate base; the required three-dimensional figure is designed through a three-dimensional design software and a corresponding model is produced by a 3D printer as an experimental device , the device mainly has a tubular structure, and the inner diameter can be realized from the micron level to the centimeter level; the 3D printing model is set on the base of the cell culture dish or the culture plate; the base of the cell culture dish or the base of the culture plate The surface was not treated, or a layer of rat tail collagen and other substances that promoted cell adhesion were incubated. Its production process does not require complicated photolithography technology, is simple, convenient and easy to operate.

[0034] figure 1 It is a schematic diagram of a 3D printing device, which is mainly composed of two parts, including an ...

Embodiment 2

[0059] This embodiment is basically the same as Embodiment 1, except that the device prepared by 3D printing technology includes two inner hollow tubes.

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Abstract

The invention belongs to the technical field of a 3D printing technique and cell culture and specifically relates to a device and method for quickly performing cell patterning co-culture based on 3D printing. The invention provides a micro device technique for the cell culture and cell patterning co-culture by utilizing 3D printing to quickly prepare. The device can be conveniently compatible with the present cell biological technique, such as, micro-imaging and cell immune staining. The patterning of various cells can be conveniently realized, the cells can be subjected to micro-imaging analysis and immunohistochemical analysis, and a quantification result can be obtained. The device and the method are supplied for researchers to quickly and conveniently realize the co-culture of multiple cells.

Description

technical field [0001] The invention belongs to the field of 3D printing technology and cell culture technology. Specifically, the invention relates to a cell patterned co-culture device and method that can be quickly prepared on the basis of 3D printing. Background technique [0002] The main purpose of cell patterning is to make cells grow in a predetermined area and form a specific pattern. Micropatterning technology can control the spatial distribution of cells at the micron scale, so it is of great significance to the research of tissue engineering, biosensor technology, and basic biological problems. Micropatterning technology can directly regulate the size of cell colonies, the distance between colonies, and the interaction between homogeneous or heterogeneous cells, and the control of these spatial parameters can directly affect cell metabolism and cell function. For example, the size and space occupied by human umbilical vein endothelial cells directly affects thei...

Claims

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Application Information

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IPC IPC(8): C12M3/00C12N5/02C12N5/077C12N5/09
CPCC12M25/06C12M33/00C12N5/0656C12N5/0693
Inventor 赵亮刘杨刘莹莹潘文杰张学记
Owner UNIV OF SCI & TECH BEIJING
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