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Device and co-culture method for different three-dimensional cell cluster pairing

A three-dimensional cell and co-culture technology, which is applied in tissue cell/virus culture devices, 3D culture, tissue culture, etc., to avoid waste of time and money, good cell compatibility and environmental friendliness, and improve work efficiency Effect

Active Publication Date: 2018-11-02
UNIV OF SCI & TECH BEIJING
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0008] The invention of using microwell array microfluidic chip device to prepare heterogeneous three-dimensional cell aggregates and make them 1:1 paired, to study the fusion reaction of heterogeneous aggregates and to apply it to drug screening has not been reported yet.

Method used

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  • Device and co-culture method for different three-dimensional cell cluster pairing
  • Device and co-culture method for different three-dimensional cell cluster pairing
  • Device and co-culture method for different three-dimensional cell cluster pairing

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Embodiment 1

[0041] Such as figure 1 As shown, this embodiment provides a device for rapidly preparing different three-dimensional cell clumps and enabling 1:1 pairing and co-cultivation of heterogeneous cell clumps, including: a microwell array chip device and a cell culture dish or culture plate Substrate; micro-column arrays are made on silicon wafers by photolithography, polydimethylsiloxane is poured on the array and heated to cure it, and after polydimethylsiloxane is peeled off from the silicon wafers, polydimethylsiloxane Arrays of microwells are formed on the base silicone. After microwell array polydimethylsiloxane chips and cofferdams were assembled, they were placed on cell culture dishes, and a layer of surfactant such as pluronic F127 (ethylene oxide-epoxy Propane-ethylene oxide block copolymer) and other substances that prevent cell attachment, and then promote cells to clump together in the microwell.

[0042] figure 1 It is a physical picture of the microwell array chip...

Embodiment 2

[0056] This embodiment is basically the same as Embodiment 1, except that the diameter of the microwell in the second device is 200 μm, and the depth is 200 μm.

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Abstract

The invention belongs to the technical field of microfluidics and cell three-dimensional culture, and particularly relates to a device and a method capable of rapidly preparing different three-dimensional cell clusters based on a simple microwell array microfluidic chip and ensuring 1:1 paring and co-culture among heterogeneous cell clusters. The device can be used for preparing the different three-dimensional cell clusters and realize the 1:1 pairing and the co-culture among the heterogeneous cell clusters; the device comprises microfluidic chips with microwell arrays and cofferdams, the periphery of each microfluidic chip is equipped with one cofferdam, and the two are tightly sealed together; the microfluidic chips with the microwell arrays and the cofferdams are all arranged on cell culture apparatus; the three-dimensional culture of a variety of cells, and the 1:1 pairing and the co-culture among the heterogeneous clusters can be conveniently realized, and microscopic imaging analysis and drug screening on cells can be performed to obtain quantitative results.

Description

technical field [0001] The present invention mainly belongs to the field of microfluidic chip technology and cell 3D culture technology, and specifically relates to a rapid preparation of different three-dimensional cell clusters based on microfluidic chip technology and the 1:1 pairing and co-cultivation of heterogeneous cell clusters devices and methods. Background technique [0002] Cell culture refers to a method of cell biology research that separates tissues or cells from organisms and makes them survive and grow under in vitro conditions that simulate the physiological environment in vivo. A technique commonly used in research in many disciplines. The two-dimensional monolayer cell culture mode of one or more cells is very convenient, and the cells also have good viability. However, tissues and organs in the body are three-dimensional structures, which are continuously perfused by the blood circulation system. Therefore, how to better simulate the physiological env...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12M3/00C12M1/00C12N5/00
CPCC12M23/16C12N5/0062
Inventor 赵亮刘莹莹刘杨张学记
Owner UNIV OF SCI & TECH BEIJING
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