105results about How to "Promotes Adhesive Growth" patented technology

The invention relates to a bracket material used for an osseous tissue project, and a preparation method thereof. Firstly, the bracket of a type I collagen is extracted from a small fresh pigskin by a mature extracting technique. Then, the bracket is further expanded in a Tris cushion liquid, the pH of which is equal to 8.8 to obtain a natural porous collagen bracket by the treatments of freezing and drying. The bracket is respectively and repeatedly mineralized in a CaCl2 liquid and in (NH4)2HPO4 liquid or mineralized in simulated body fluid for a long period to lead the weakly crystallized HA to be uniformly settled into the collagen bracket; and then a pigskin collagen-hydroxyapatite ossein is obtained by the treatments of freezing and drying to replace the natural bracket material. The invention not only maintains the natural bracket structure of the collagen in an organism, but also has the advantages of low material cost, simple devices, short period and easy operation. The obtained compound bracket material used for the pigskin collagen-hydroxyapatite osseous tissue project has the characteristics of high intensity, large toughness, non-antigenicity, higher biological activity as well as degradation and releasing control.

The invention discloses a three-dimensional single cell culture chip realized based on a micro-fabrication technology, and belongs to bio micro electro mechanical systems (Bio-MEMS). The chip comprises cavities distributed in an array; adjacent cavities are connected with each other through a micro groove; the depth of the micro groove is consistent with the height of the cavities; and the bottoms of the cavities are provided with nano projections. According to difference of adhesion of cells on the surface of blank polydimethylsiloxane (PDMS) and on the surface of PDMS with a nano micro morphology structure and by combining a surface topology pattern, the chip realizes cell patterning culture. The chip can be used for cell culture without chemical or biological reagents, overcomes a defect of low stability of the conventional patterning method, has the characteristics of durable and stable pattern, controllable preparation process and the like, and can meet the requirement of batch production. Meanwhile, a patterning design that the cavities are connected through micro grooves is adopted, and the research on the interaction of cells can be realized.

The invention discloses a chitosan/polylysine in-situ gel and a preparation method thereof. The gel is an in-situ gel prepared from 1-5.5 percent by weight of sulfhydrylization chitosan and 0.1- 3.2 percent by weight of maleimide polylysine. The preparation method comprises the following steps of synthetizing the polylysine containing maleimide double bonds, and preparing a maleimide polylysine solution by taking a PBS (phosphate buffer solution) as a solvent; preparing a sulfhydrylization chitosan solution by taking the PBS as a solvent; and uniformly mixing the two solutions under the condition of physiological pH value to obtain the in-situ gel. The invention has the advantages that the preparation process is simple, and the prepared in-situ gel simulates the components, the structure and the functional characteristics of polysaccharide/polypeptide of a natural extracellular substrate, helps to increase histocompatibility of a material, promotes cell adhesion growth and has wide application prospects in the fields of tissue engineering and drug release.

The present invention discloses an injectable-porous-drug loaded polymethyl methacrylate-based composite scaffold bone transplant material and a preparation method thereof, and belongs to the field of organic functional materials preparation. The polymethyl methacrylate-based composite scaffold bone transplant material uses polymethyl methacrylate (PMMA) as a scaffold for providing mechanical supporting, and a chitosan-based thermosensitive hydrogel as a pore forming agent and an osteoconductive material and a drug carrier, and the polymethyl methacrylate (PMMA) and the chitosan-based thermosensitive hydrogel are mixed with each other to form an injectable-porous three-dimensional structural bone cement composite. The scaffold bone transplant material is simple in preparation method, suitable in reaction temperature, and good in biocompatibility, has matched mechanical properties, good biological mineralization and corresponding anti-bacterial, anti-inflammatory or anti-tumor capabilities, and has broad prospects in clinical application of reconstruction of bone tissues in future.

The invention discloses a cell culture plate based on oriented polymer nanometer fiber and a preparation method thereof. The cell culture plate comprises a standard flat-bottom cell culture plate used as a main body and a polymer nanometer fiber support adhered on the hole bottom of the culture plate. The polymer nanometer fiber support is prepared by adding bioactive components into the main raw material--polymer, carrying out grafting or blending, then carrying out electrostatic spinning and carrying out collection by using a fiber collecting device of a parallel conductive panel; and fiber bundles on the polymer nanometer fiber support are of a regular oriented arrangement structure. The culture plate provided by the invention has good biocompatibility and is applicable to culture of cells or tissue with oriented morphological characteristics, especially to culture and regeneration of cells and related tissue of nerves, bones, muscles, ligaments, etc.

The invention discloses a composite artificial cornea and a preparation method thereof. The composite artificial cornea comprises a central optical pillar prepared from polyhydroxyethyl methacrylate (PHEMA), a corneal skirt structure prepared from an acellular stromal hydrogel, and an intermediate sandwich grid scaffold connecting the central optical pillar and the corneal skirt structure, whereinthe corneal skirt structure is provided with a nerve cell scaffold layer to which cells are adhered and a corneal stromal cell scaffold layer. The composite artificial cornea is simple in structure,good in biocompatibility, easy to process, can well simulate the environment of cornea in vivo, and is favorable for transplantation.

Provided is a cervical pedicle screw feeding guiding formwork. The formwork surface of the guiding formwork is a profiling faying surface of a spinous process area on a single section cervical vertebra; the back side of the faying surface of the guiding formwork is provided with a handle; corresponding to a spinous process bump of the spinous process area, a limiting through hole penetrates the handle and the guiding formwork, a limiting tail pin is movably installed in the limiting through hole, and the tip of the limiting tail pin is embedded into and penetrates the spinous process bump; corresponding to pedicles on the two sides of the spinous process area, bosses are protruded on the back side of the faying surface of the guiding formwork respectively, the bosses are provided with hollow access through holes, and sleeves are movably installed in the hollow access through holes. The sleeves are medical resin sleeves. The guiding formwork has a sufficient faying area and faying stability, the shaking of the guiding formwork relative to a vertebral plate during an operation is effectively prevented, the pin feeding accuracy can be guaranteed for different patients, and the operation difficulty and the operation risk are lowered. The medical resin sleeves are high in consistency degree with the human tissue, pin feeding guiding for double-side or single-side vertebral body guiding can be achieved.

The invention discloses a method for preparing a biomimetic blood vessel stent by composite technology, a blood vessel stent with three-layer structure is prepared by adopting coaxial electrospinningtechnology and coaxial extrusion hollow fiber technology in the field of biological manufacture, and each layer of the blood vessel scaffold can be inoculated with different types of cells between thethree layers of the blood vessel stent, so that the blood vessel stent has a true biomimetic human blood vessel structure; the inner and outer layers can be loaded with drugs, and the middle layer can provide oxygen, nutrients and exclude metabolites for cells. It is helpful to the growth of blood vessel and shortens the period of blood vessel growth, so it has a broad prospect of clinical application.

The invention relates to the field of biomaterials, and particularly relates to a method for preparing PEEK (Polyetheretherketone) biological coatings and a PEEK biomaterial. The PEEK, of which the surface is adhered with polydopamine, is put in a titanium fluoride solution; heat insulation is carried out for 10 hours to 15 hours at 45 DEG C to 55 DEG C and titanium fluoride is deposited; after the surface of the PEEK is adhered with the polydopamine, a titanium fluoride compounded coating is deposited; by using phenolic hydroxyl in the polydopamine as an anchoring point of titanium dioxide, the hybrid compounded coating is prepared through liquid phase deposition. Compared with the prior art, the compounded coating prepared by adopting the method disclosed by the invention has the advantages that the polydopamine and TiO2 are bonded through powerful chemical bonds, so that the bonding effect is very good. The titanium dioxide has an antibacterial effect, so that the finally prepared compounded coating has excellent antibacterial performance. Due to the polydopamine, adhesive growth of the fibroblasts can be promoted, and adhesion, growth and differentiation of the fibroblasts canbe effectively promoted; and finally, the sealing ability of soft tissues is achieved.

The invention belongs to the technical field of medical biological materials and discloses a compound biological patch for repairing abdominal wall defects. The compound biological patch is prepared from a biological patch layer and a bletilla striata fiber mesh layer which covers the surface of one side of the biological patch layer, wherein the bletilla striata fiber mesh layer is an electrospunnano-grade bletilla striata fiber mesh. According to the compound biological patch disclosed by the invention, the bletilla striata fiber mesh layer is combined with a traditional biological patch coating film, so that the anti-infection capability and the wound repairing capability of the compound biological patch are effectively improved; meanwhile, after the compound biological patch is implanted into an organism, the mechanical strength change is higher than needed mechanical strength all the time and tissue reconstruction can be accelerated; existing technical bottlenecks of the biological patch are conducive to solving and the treatment effect of repairing the abdominal wall defects is effectively improved.

The invention aims to provide an artificial soft tissue braided fabric which has excellent mechanical properties, such as dynamic properties and facilitates cell growth and a preparation method for the braided fabric. The artificial soft tissue braided fabric disclosed by the invention comprises a first surface layer, a first middle layer, an inner layer, a second middle layer and a second surface layer in turn; the first surface layer and the second surface layer are locally or wholly prepared from silks; the first middle layer and the second middle layer are locally or wholly prepared from PET; and the inner layer is locally or wholly prepared from PEHM.

The invention relates to a method for preparing polypeptide copolymer porous nanofiber by using electrostatic spinning. The method comprises the following steps: firstly, carrying out hydrophilic modification on a hydrophobic polypeptide homopolymer to prepare a poly(gamma-benzyl L-glutamate-hydroxyethyl glutamine) or poly(gamma-benzyl L-glutamine)-g-polyethylene glycol grafted copolymer; secondly, preparing a compound solvent by using solvents with different volatilization properties, and then preparing into a spinning solution; thirdly, spinning by using a high voltage electrostatic spinning method; and finally, placing spun yarns into a vacuum drying chamber and drying for more than 3 hours at room temperature to obtain the porous nanofiber with high specific surface area. A cytotoxicity test indicates that the prepared porous nanofiber with high specific surface area is free of cytotoxicity and good in biocompatibility, can be used as a porous scaffold for cell growth, has the function of promoting cell migration and amplification and has a relatively good application value in the aspect of preparation of scaffolds for tissue engineering; besides, the polypeptide copolymer porous nanofiber has an application prospect in the fields of medical dressing, medicine released control, artificial organs, sewage treatment and the like.

The invention provides biodegradable polymer microparticles and a preparation method thereof. The polymer microparticles is a copolymer of lactic acid and/or glycolic acid repeating unit, and the particle sizes of the polymer microparticles are 10 micrometers to 150 micrometers. The PLLA microparticles disclosed by the invention has an irregular, unsmooth or rough microscopic shape, so that the contact area of the PLLA particles and cells is increased, the adhesion ability and the retention time of the cells on a porous structure or a scaffold structure are improved and prolonged, and the cellaffinity performance of the PLLA particles is obviously improved; the biodegradable polymer microparticles are beneficial for stimulating collagenocyte to feel physical and mechanical micro-environment stimulation and make a response, so that growth of collagen of a human body is further stimulated, and the effects of removing wrinkles, maintaining beauty and delaying aging are natural and lasting.

The invention discloses a 3D printing scaffold material for icariin (ICA)-loaded PLGA microspheres, wherein the 3D printing scaffold material can be used for skull defect repairing. An ICA functionaldrug is adopted, endogenous osteoblasts are collected to a skull defect area to prompt osteoblast differentiation of the endogenous osteoblasts, meanwhile based on 3D printing design microspheres anda nano-hydroxyapatite micro-nano structure composite scaffold, an individualized bionic degradable polymer scaffold is prepared and can be absorbed and degraded in human bodies to avoid side effects of second operation and implantation materials, the effects of bone conduction and bone induction are achieved, bone tissue regeneration is prompted, and bone healing is prompted.

The invention discloses a preparation method of a polyhydroxyalkanoate/polypyrrole composite electrospun membrane and an electrospinning membrane; the preparation method comprises the steps of preparing a polyhydroxyalkanoate fiber electrospun membrane through an electrospinning process, and then preparing the polyhydroxyalkanoate/polypyrrole composite electrospun membrane through a surface modification process. The preparation method is simple and rapid, raw materials are easy to obtain, and the prepared polyhydroxyalkanoate/polypyrrole composite electrospun membrane has good hydrophilicity and cell compatibility, can provide durable and stable skeleton structure supporting after being implanted into tissues, can regulate cell attachment and cell migration and induce tissue differentiation under electrical stimulation, and has wide application prospects in the bioengineering fields such as oriented repair of tissue.

The invention relates to new use of carbonized cuttlebones, in particular to application of carbonized cuttlebones in preparation of medical materials for treating diseases of bone defect, bone tumor and cyst. The medical materials can be bone tissue engineering scaffold materials or bone grafting substitution materials. The invention has the advantages that: the carbonized cuttlebones have excellent microporous structure and biocompatibility, is beneficial to osteoblast adherence growth and promotes bone differentiation to a certain extent. The microwave carbonized cuttlebones can be used as the bone tissue engineering scaffold materials or bone grafting substitution materials, therefore, the invention is hopeful to be used for clinical treatment and repairing of dead space after the cutting of bone defect, bone tumor and cyst, promotes combination of vertebral column and joints, and can be used as a cell carrier to construct tissue-engineered bones in vitro in a compounding way.