3D printing scaffold material for icariin (ICA)-loaded PLGA microspheres and application of 3D printing scaffold material

A technology of icariin and 3D printing, which can be used in additive processing, medical science, tissue regeneration, etc., can solve the problems that limit the wide development and application of stem cell transplantation, the difficulty of separation and purification, and the lack of cell sources, so as to promote adhesion The effect of growth, simple operation, and no side effects

Inactive Publication Date: 2019-12-17
FUZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the difficulty in obtaining materials, the lack of cell sources, and the difficulty in separation and purification all severely limit the extensive development and application of stem cell transplantation in periodontal treatment in the future.

Method used

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  • 3D printing scaffold material for icariin (ICA)-loaded PLGA microspheres and application of 3D printing scaffold material
  • 3D printing scaffold material for icariin (ICA)-loaded PLGA microspheres and application of 3D printing scaffold material
  • 3D printing scaffold material for icariin (ICA)-loaded PLGA microspheres and application of 3D printing scaffold material

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] (1) Prepare 250ml of polyvinyl alcohol (PVA) solution with a concentration of 0.3% and 10ml with a concentration of 4%;

[0043] The preparation method is as follows: Weigh the powder in a certain volume of deionized water, let it stand for 2 hours, heat it at 45°C for 2 hours, heat it at 60°C for 2 hours, and heat it at 90°C for 4 hours to completely dissolve it.

[0044] (2) Dissolve 0.5g PLGA in 5ml dichloromethane;

[0045] (3) Add 250ul to (2) with a concentration of 10 -5 mol / L ICA solution, ultrasonic treatment emulsion;

[0046] (4) Add 10ml of 4% PVF to (3) to form a second emulsion, vortex and sonicate to obtain a homogeneous emulsion;

[0047] (5) Add the prepared homogeneous emulsion into 250ml of 0.3% PVF solution, stir to evaporate the solvent, centrifuge, wash, and freeze-dry;

[0048] (6) Put the PLGA microspheres loaded with icariin at a mass fraction of 5%, polycaprolactone at a mass fraction of 85%, and nano-hydroxyapatite at a mass fraction of 10%...

Embodiment 2

[0050] (1) Prepare 250ml of polyvinyl alcohol (PVA) solution with a concentration of 0.3% and 10ml with a concentration of 4%;

[0051] The preparation method is as follows: Weigh the powder in a certain volume of deionized water, let it stand for 2 hours, heat it at 45°C for 2 hours, heat it at 60°C for 2 hours, and heat it at 90°C for 4 hours to completely dissolve it.

[0052] (2) Dissolve 0.5g PLGA in 5ml dichloromethane;

[0053] (3) Add 250ul to (2) with a concentration of 10 -5 mol / L ICA solution, ultrasonic treatment emulsion;

[0054] (4) Add 10ml of 4% PVF to (3) to form a second emulsion, vortex and sonicate to obtain a homogeneous emulsion;

[0055] (5) Add the prepared homogeneous emulsion into 250ml of 0.3% PVF solution, stir to evaporate the solvent, centrifuge, wash, and freeze-dry;

[0056] (6) Put the PLGA microspheres loaded with icariin with a mass fraction of 8%, polycaprolactone with a mass fraction of 82%, and nano-hydroxyapatite with a mass fraction ...

Embodiment 3

[0058] (1) Prepare 250ml of polyvinyl alcohol (PVA) solution with a concentration of 0.3% and 10ml with a concentration of 4%;

[0059] The preparation method is as follows: Weigh the powder in a certain volume of deionized water, let it stand for 2 hours, heat it at 45°C for 2 hours, heat it at 60°C for 2 hours, and heat it at 90°C for 4 hours to completely dissolve it.

[0060] (2) Dissolve 0.5g PLGA in 5ml dichloromethane;

[0061] (3) Add 250ul to (2) with a concentration of 10 -5 mol / L ICA solution, ultrasonic treatment emulsion;

[0062] (4) Add 10ml of 4% PVF to (3) to form a second emulsion, vortex and sonicate to obtain a homogeneous emulsion;

[0063] (5) Add the prepared homogeneous emulsion into 250ml of 0.3% PVF solution, stir to evaporate the solvent, centrifuge, wash, and freeze-dry;

[0064] (6) Put the PLGA microspheres loaded with icariin at a mass fraction of 10%, polycaprolactone at a mass fraction of 80%, and nano-hydroxyapatite at a mass fraction of 10...

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Abstract

The invention discloses a 3D printing scaffold material for icariin (ICA)-loaded PLGA microspheres, wherein the 3D printing scaffold material can be used for skull defect repairing. An ICA functionaldrug is adopted, endogenous osteoblasts are collected to a skull defect area to prompt osteoblast differentiation of the endogenous osteoblasts, meanwhile based on 3D printing design microspheres anda nano-hydroxyapatite micro-nano structure composite scaffold, an individualized bionic degradable polymer scaffold is prepared and can be absorbed and degraded in human bodies to avoid side effects of second operation and implantation materials, the effects of bone conduction and bone induction are achieved, bone tissue regeneration is prompted, and bone healing is prompted.

Description

technical field [0001] The invention belongs to the field of composite materials, and in particular relates to a 3D printing support material of PLGA microspheres loaded with icariin and its application. Background technique [0002] Bone is the main organ of the movement system of the human body and animals. It is mainly composed of bone tissue (including bone cells, collagen fibers and matrix, etc.), has a certain shape and structure, is covered with periosteum, contains bone marrow, and contains rich blood vessels and lymphatic vessels. And nerves, constantly undergoing metabolism and growth and development, have the ability to repair, regenerate and rebuild after trauma. As a bone scaffold, it endows the bone with good toughness and elasticity and can effectively regulate the crystallization and growth of inorganic minerals; the inorganic matter accounts for about 65% of the bone weight, and the main component is nano-hydroxyapatite (Ca 10 (PO 4 ) 6 (OH) 2 , nHAP) gr...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/18A61L27/12A61L27/54A61L27/58B33Y10/00B33Y70/00
CPCA61L27/12A61L27/18A61L27/54A61L27/58A61L2300/232A61L2300/412A61L2300/602A61L2430/02B33Y10/00B33Y70/00C08L67/04
Inventor 陈景帝邹琳耿玉生徐敢陈立
Owner FUZHOU UNIV
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