Chitosan/polylysine in-situ gel and preparation method thereof

A technology of polylysine and in situ gel is applied in the field of biomedical materials to achieve the effects of controllable reaction, simple preparation process and increased histocompatibility

Inactive Publication Date: 2012-07-18
TIANJIN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] Natural polypeptides with multi-side functionalities are used as cross-linking agents to simulate the protein composition and functional characteristics of the extracellular matrix, and chitosan-based gels are synthesized in situ by Michael addition to enhance the biocompatibility and cell adhesion of the gels. Adhesion, the in situ gel and its preparation method have not been reported yet

Method used

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  • Chitosan/polylysine in-situ gel and preparation method thereof
  • Chitosan/polylysine in-situ gel and preparation method thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0022] Weigh 14mg of succinimide sulfate, dissolve in 8ml of PBS with pH=7.2~7.4, stir until a clear solution is formed; weigh 30mg of e -Polylysine, completely dissolved in 2ml of PBS with pH=7.2~7.4, stirred until a clear solution was formed; at room temperature, the above polylysine solution was added to the succinimide sulfate solution, at a stirring rate of 50rpm Mix well, react for 0.5 hour, dialyze with deionized water at room temperature for 24 hours, and freeze-dry the dialyzed product to obtain maleimidated polylysine with a double bond molar substitution degree of 5%. Dissolving the maleimidated polylysine powder in PBS with a pH value of 7.2 to 7.4 to prepare a maleimidated polylysine solution with a concentration of 40 mg / ml;

[0023] Weigh 1g chitosan ( =1 zero.5×10 5 , the degree of deacetylation is 92%) was dissolved in 90ml of deionized water to form a suspension, 0.8g of 1-hydroxybenzotriazole was added to the above suspension, stirred to obtain a clear so...

Embodiment 2

[0026] Weigh 30mg of succinimide sulfate, dissolve in 16ml of PBS with pH=7.2~7.4, stir until a clear solution is formed; weigh 31mg of e-Polylysine, completely dissolved in 4ml of PBS with pH=7.2~7.4, stirred until a clear solution was formed; at room temperature, the above polylysine solution was added to the succinimide sulfate solution, at a stirring rate of 100rpm Mix well, react for 2 hours, dialyze with deionized water at room temperature for 24 hours, and freeze-dry the dialyzed product to obtain maleimidated polylysine with a double bond molar substitution of 30%. Dissolving the maleimidated polylysine powder in PBS with a pH value of 7.2 to 7.4 to prepare a maleimidated polylysine solution with a concentration of 40 mg / ml;

[0027] Thiolated chitosan with a degree of mercapto substitution of 150 μmol / g ( =1.5×10 5 , deacetylation degree is 92%) chitosan is dissolved in phosphate buffer saline (PBS) with a pH value of 7.2~7.4 to prepare a thiolated chitosan solutio...

Embodiment 3

[0030] Weigh 20mg of succinimide sulfate, dissolve in 11ml of PBS with pH=7.2~7.4, stir until a clear solution is formed; weigh 30mg of e -Polylysine, completely dissolved in 3ml of PBS with pH=7.2~7.4, stirred until a clear solution was formed; at room temperature, the above-mentioned polylysine solution was added to the succinimide sulfate solution, at a stirring rate of 80rpm Mix well, react for 1 hour, dialyze with deionized water at room temperature for 24 hours, and freeze-dry the dialyzed product to obtain maleimidated polylysine with a double bond molar substitution degree of 15%. Dissolving the maleimidated polylysine powder in PBS with a pH value of 7.2 to 7.4 to prepare a maleimidated polylysine solution with a concentration of 40 mg / ml;

[0031] Thiolated chitosan with a degree of mercapto substitution of 160 μmol / g ( =1.2×10 5 , deacetylation degree is 85%) chitosan is dissolved in phosphate buffer saline (PBS) with a pH value of 7.2~7.4 to prepare a thiolated ...

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Abstract

The invention discloses a chitosan/polylysine in-situ gel and a preparation method thereof. The gel is an in-situ gel prepared from 1-5.5 percent by weight of sulfhydrylization chitosan and 0.1- 3.2 percent by weight of maleimide polylysine. The preparation method comprises the following steps of synthetizing the polylysine containing maleimide double bonds, and preparing a maleimide polylysine solution by taking a PBS (phosphate buffer solution) as a solvent; preparing a sulfhydrylization chitosan solution by taking the PBS as a solvent; and uniformly mixing the two solutions under the condition of physiological pH value to obtain the in-situ gel. The invention has the advantages that the preparation process is simple, and the prepared in-situ gel simulates the components, the structure and the functional characteristics of polysaccharide/polypeptide of a natural extracellular substrate, helps to increase histocompatibility of a material, promotes cell adhesion growth and has wide application prospects in the fields of tissue engineering and drug release.

Description

technical field [0001] The invention relates to a chitosan / polylysine in-situ gel and a preparation method thereof, belonging to the technical field of biomedical materials. Background technique [0002] Hydrogels are widely used as biomedical materials due to their similar characteristics to natural extracellular matrix. Among them, the in situ gel can be quickly converted from a liquid to a gel-like substance in situ, which has the incomparable advantages of general hydrogels, and can avoid highly traumatic surgical procedures, reduce patient pain, and reduce medical expenses. Can be used to repair complex, irregularly shaped tissues. If it is directly used in human contact, it can prevent the infection of microorganisms outside the body, inhibit the loss of body fluids, and at the same time promote wound healing. Therefore, the hydrophilic three-dimensional network structure of the in situ gel and the unique solution-gel transition properties make it easy to prepare, e...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08L5/08C08L77/04C08J3/075A61L24/08A61L27/52A61L27/54A61K47/36
Inventor 聂微赵瑾袁晓燕
Owner TIANJIN UNIV
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