Cerebellar decellularized regeneration biological scaffold, preparation method and application thereof

A decellularized scaffold and bio-scaffold technology, applied in medical science, prosthesis, etc., can solve problems such as no bio-scaffold, achieve good biocompatibility, and promote the formation of extracellular matrix.

Inactive Publication Date: 2017-05-24
AFFILIATED HUSN HOSPITAL OF FUDAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

So far, there have been no reports of in vitro regeneration of central nervous system (central nervous system, CNS) tissues and organs at home and abroad, and no reports of ideal CNS biological scaffolds

Method used

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  • Cerebellar decellularized regeneration biological scaffold, preparation method and application thereof
  • Cerebellar decellularized regeneration biological scaffold, preparation method and application thereof
  • Cerebellar decellularized regeneration biological scaffold, preparation method and application thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0047] Example 1 Preparation of Scaffold for Cerebellar Regeneration

[0048] 1. Preparation and Characterization of Scaffolds for Cerebellar Regeneration

[0049] 1) Cerebellum sampling

[0050] Healthy adult C57BL / 6 mice (body weight 20-25g, male or female) were intraperitoneally anesthetized with 10% chloral hydrate, and then perfused with 1.0% SDS (sodium dodecyl sulfonate, Sodium Dodecyl Sulfonate) for 5 minutes (20ml / min). The prone position was fixed, the skin was prepared routinely, and the scalp was incised along the midline to expose the skull. The skull was cut open, the cerebellum was completely removed, and the movements were gentle. In order to ensure sterility, the subsequent operations were performed on a sterile operating table. After cleaning with PBS, carefully separate and remove all non-cerebellar tissues in PBS.

[0051] 2) Decellularization process of mouse cerebellum

[0052] Follow-up is a series of stirred water bath processes at room temperature...

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Abstract

Belonging to the field of biological tissue engineering and medical materials, the invention relates to a decellularized cerebellar extracellular matrix regeneration biological scaffold containing multiple neurotrophic factors and a preparation method thereof. As a cerebellum regeneration scaffold material, the natural cerebellar decellularized scaffold adopted by the invention is a three-dimensional network structure, and the spatial configuration conforms to the cerebellar physiological structure spatial configuration. The regeneration biological scaffold and its biomechanical properties are similar to normal cerebellus, and can provide physiologically similar channels for reconstruction of nerve pathways so as to provide correct guidance for growth. The scaffold surface has cell-membrane receptor recognition sites and partially active factors, thus being conducive to adhesion growth of cells and functioning of physiological functions. Engineered seed cells can secrete a variety of neurotrophic / growth factors, also can promote the formation of new extracellular matrixes, and provides regeneration guidance signals. With good biocompatibility, the regeneration biological scaffold can be used for preparation of neural repair preparations for treatment of nerve injuries.

Description

technical field [0001] The invention belongs to the field of biological tissue engineering and medical materials, and relates to a cerebellum decellularized regeneration biological scaffold and its preparation method and application, in particular to a cerebellum extracellular matrix regeneration biological scaffold containing various neurotrophic factors after removing cells and a preparation method thereof And the application in the preparation of nerve repairing preparation after nerve injury. Background technique [0002] According to reports, organ dysfunction caused by disease, injury and aging of human tissues and organs leads to a huge gap in clinical tissue and organ replacement therapy. In clinical practice, due to the limited source of alternative organs, how to use artificial methods to generate functional human organs in vitro has become the focus of attention all over the world. The research on bioartificial organs has become a hotspot in regenerative medicine...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/36A61L27/50
Inventor 朱侗明朱剑虹
Owner AFFILIATED HUSN HOSPITAL OF FUDAN UNIV
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