Degradation controlled tissue engineering comea fibrous scaffold and preparation method thereof

A fibrous scaffold and tissue engineering technology, which is applied in the field of degradable and controllable tissue engineered corneal fibrous scaffold and its preparation, can solve the problems of low mechanical strength and poor stability, and achieve low cost, simple preparation process, good biological activity and biophase capacitive effect

Inactive Publication Date: 2010-04-21
JILIN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

For example, the shortcomings of collagen are poor stability and low mechanical strength, and are easily degraded by collagenase. It needs to be cross-linked by various physical or chemical methods, such as thermal cross-linking, UV and lambda ray cross-linking, polycyclic Oxide cross-linking, etc., to improve mechanical strength and resistance to degradation
[0004] There are few reports on the preparation method of directly using biomaterial fibers as tissue engineering corneal scaffolds and achieving controllable degradation

Method used

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  • Degradation controlled tissue engineering comea fibrous scaffold and preparation method thereof
  • Degradation controlled tissue engineering comea fibrous scaffold and preparation method thereof
  • Degradation controlled tissue engineering comea fibrous scaffold and preparation method thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0027] In a water bath at 37°C, 7 mg of gelatin was dissolved in 10 mL of a mixed solvent of 9.9 g of hexafluoroisopropanol and 0.1 g of N,N-dimethylformamide, and then 3 mg of polylactic acid PLLA was dissolved in the above gelatin solution to prepare The spinning liquid is obtained, and the tissue engineering corneal fiber scaffold is prepared through the electrospinning forming equipment. The working voltage of the electrospinning forming equipment is 18KV; 2cm parallel plate electrodes. The obtained scaffold was vacuum-dried at 40° C. for 3 days to obtain a tissue engineered corneal fiber scaffold with controllable degradation. After performance test: light transmittance 97.83%, wet tensile strength 2.19MPa, elongation at break 956.2%, water content 154.1%, contact angle 76.5°.

[0028] figure 1 It is the scanning electron micrograph of the degradable tissue engineering corneal fiber scaffold prepared by the present embodiment; from figure 1 It can be seen that the degr...

Embodiment 2

[0030] In a water bath at 32°C, dissolve 9 mg of gelatin in 10 mL of a mixed solvent of 9.8 g of hexafluoroisopropanol and 0.2 g of N, N-dimethylformamide, and then dissolve 1 mg of polylactic acid PLLA in the above gelatin solution to obtain Spinning liquid, through electrospinning forming equipment, prepares tissue engineering corneal fiber scaffold, the operating voltage of electrospinning forming equipment is 15KV; the receiving plate distance is 12cm, the sampling rate is 1.5mL / h, and the receiving device is 20cm in diameter Round foil. The obtained scaffold was vacuum-dried at 37° C. for 2 days to obtain a tissue engineered corneal fiber scaffold with controllable degradation. After performance test: the light transmittance is 97.26%, the wet tensile strength is 1.99MPa, the elongation at break is 816.6%, the water content is 95.09.%, and the contact angle is 109.21°.

[0031] figure 2 It is the scanning electron micrograph of the degradable tissue engineering corneal...

Embodiment 3

[0033] In a water bath at 30°C, dissolve 8 mg of gelatin in 10 mL of a mixed solvent of 9.8 g of hexafluoroisopropanol and 0.2 g of N, N-dimethylformamide, and then dissolve 2 mg of polylactic acid PLA in the above gelatin solution to obtain Spinning liquid, through electrospinning forming equipment, prepare tissue engineering corneal fiber scaffold, the working voltage of electrospinning forming equipment is 20KV; the receiving plate distance is 15cm, the sampling rate is 2.0mL / h, and the receiving device is 18cm in diameter Round foil. The obtained scaffold was vacuum-dried at 30° C. for 4 days to obtain a tissue engineered corneal fiber scaffold with controllable degradation. After performance test: the light transmittance is 90.22%, the wet tensile strength is 4.72MPa, the elongation at break is 505.4%, the moisture content is 100.42.%, and the contact angle is 104.1°.

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Abstract

The invention relates to a degradation controlled tissue engineering comea fibrous scaffold and a preparation method thereof. The basic compositions of the scaffold are gelatin and aliphatic polyesters. The preparation method comprises the following steps: dissolving the gelatin in a mixed solvent to prepare gelatin solution, dissolving the aliphatic polyesters in the gelatin solution, and evenlystirring to prepare mixed polymer spinning solution; and preparing the orderly and disorderly arranged fibrous scaffold through electrostatic spinning, and performing vacuum drying to prepare the degradation controlled tissue engineering comea fibrous scaffold. The gelatin and aliphatic polyesters compounded electrostatic spinning is adopted, crosslinking stabilization is not needed, and the degradation speed of the scaffold and growth speed of corneal cells can be controlled to be synchronous. The product has transmittance of 97 percent, wet tensile strength of 4.91MPa, elongation at ruptureof 956.2 percent, water content of 154.1 percent, and performance parameters similar to human comea, and has good bioactivity and biocompatibility, has controlled degradation and no toxicity, is favorable for adhesion and growth of the cells, and has certain strength and toughness, simple preparation process, low cost and easy wide application.

Description

technical field [0001] The invention relates to a biological material in biological tissue engineering, in particular to a tissue engineering corneal fiber scaffold with controllable degradation and a preparation method. Background technique [0002] The cornea is the "sweeping lens" of the eye, which gathers light and creates an image on the retina at the back of the eye. Severe vision loss or blindness can result if the cornea is swollen or damaged from disease, physical injury, etc. Because corneal endothelial cells cannot repair automatically, corneal transplantation is currently an effective method for treating corneal blindness. At present, the commonly used treatment method is allogeneic cornea or animal corneal transplantation, which has the problems of insufficient donor sources, immune rejection between allogeneic and new wounds. Therefore, manufacturing a precise and effective artificial cornea that will not be rejected by the human body is a major challenge in ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/40A61L27/56A61L27/58A61L27/22A61L27/18
Inventor 王洪艳颜静崔学军李俊峰
Owner JILIN UNIV
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