Degradation controlled tissue engineering comea fibrous scaffold and preparation method thereof

Abstract

The invention relates to a degradation controlled tissue engineering comea fibrous scaffold and a preparation method thereof. The basic compositions of the scaffold are gelatin and aliphatic polyesters. The preparation method comprises the following steps: dissolving the gelatin in a mixed solvent to prepare gelatin solution, dissolving the aliphatic polyesters in the gelatin solution, and evenlystirring to prepare mixed polymer spinning solution; and preparing the orderly and disorderly arranged fibrous scaffold through electrostatic spinning, and performing vacuum drying to prepare the degradation controlled tissue engineering comea fibrous scaffold. The gelatin and aliphatic polyesters compounded electrostatic spinning is adopted, crosslinking stabilization is not needed, and the degradation speed of the scaffold and growth speed of corneal cells can be controlled to be synchronous. The product has transmittance of 97 percent, wet tensile strength of 4.91MPa, elongation at ruptureof 956.2 percent, water content of 154.1 percent, and performance parameters similar to human comea, and has good bioactivity and biocompatibility, has controlled degradation and no toxicity, is favorable for adhesion and growth of the cells, and has certain strength and toughness, simple preparation process, low cost and easy wide application.

Description

technical field

[0001] The invention relates to a biological material in biological tissue engineering, in particular to a tissue engineering corneal fiber scaffold with controllable degradation and a preparation method. Background technique

[0002] The cornea is the "sweeping lens" of the eye, which gathers light and creates an image on the retina at the back of the eye. Severe vision loss or blindness can result if the cornea is swollen or damaged from disease, physical injury, etc. Because corneal endothelial cells cannot repair automatically, corneal transplantation is currently an effective method for treating corneal blindness. At present, the commonly used treatment method is allogeneic cornea or animal corneal transplantation, which has the problems of insufficient donor sources, immune rejection between allogeneic and new wounds. Therefore, manufacturing a precise and effective artificial cornea that will not be rejected by the human body is a major challenge in ...

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