Polydimethylsiloxane (PDMS)-based three-dimensional single cell culture chip and controllable preparation method thereof
A single-cell, three-dimensional technology, applied in tissue cell/virus culture devices, biochemical equipment and methods, bioreactors/fermenters for specific purposes, etc., can solve problems such as poor pattern shape and pattern property stability , to achieve long-term stability of pattern properties and pattern structure
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[0023] Example 1:
[0024] The chip in this example is used to culture osteoblast MC3T3-E1 for cell contact connection research. MC3T3-E1 average diameter 20μm, average height h 0 3μm, average synapse length l 0 It is 20μm.
[0025] Refer to Figure 1 ~ Figure 3 . The three-dimensional single-cell culture chip used for MC3T3-E1 culture in this embodiment includes circular cavities 1 distributed in an array. There are microgrooves 3 connected between adjacent cavities 1, and the depth of the microgrooves 3 is the same as that of the cavity. The height h of body 1 is 4μm; the bottom of cavity 1 contains nano-protrusions; the diameter of cavity 1 The distance l between two adjacent cavities 1 is 50 μm; the micro grooves 3 are uniformly distributed at 90°, and the width of the micro grooves 3 is a=2 μm.
[0026] The preparation method of the PDMS-based three-dimensional single cell culture chip in this embodiment is completed by MEMS technology and replication molding technology, an...
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[0040] Example 2:
[0041] The chip in this example is used to cultivate human breast epithelial cells HMEC for cell non-contact connection research. HMEC average diameter 45μm, average height h 0 5μm, average synapse length l 0 It is 10μm.
[0042] Refer to Figure 1 ~ Figure 3 . The three-dimensional single cell culture chip used for HMEC culture in this embodiment includes circular cavities 1 distributed in an array. There are microgrooves 3 connected between adjacent cavities 1. The height h is h=7μm; the bottom of cavity 1 contains nano-protrusions; the diameter of cavity 1 The distance between two adjacent cavities 1 is l=80 μm; the micro grooves 3 are uniformly distributed at 90°, and the width of the micro grooves 3 is a=5 μm.
[0043] The preparation method of the PDMS-based three-dimensional single cell culture chip in this embodiment is completed by MEMS technology and replication molding technology, and specifically includes the following steps:
[0044] Step 1: Mix t...
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