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Electrostatic spinning nanofiber-extracellular matrix composite material as well as preparation method and application thereof

A technology of electrospinning and nanofibers, which is applied in the field of electrospinning nanofibers-extracellular matrix composite materials and its preparation, can solve the problems of ineffective resistance to soft tissue pressure and poor mechanical properties, and achieve high application prospects and Practical value, good mechanical properties, enhanced strength effect

Inactive Publication Date: 2014-06-25
SUN YAT SEN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, purely using extracellular matrix as a tissue engineering repair material has disadvantages such as poor mechanical properties and inability to effectively resist the pressure of soft tissues.

Method used

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  • Electrostatic spinning nanofiber-extracellular matrix composite material as well as preparation method and application thereof
  • Electrostatic spinning nanofiber-extracellular matrix composite material as well as preparation method and application thereof
  • Electrostatic spinning nanofiber-extracellular matrix composite material as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] 1. Preparation of electrospun fiber membranes of biodegradable materials:

[0035] (1) Weigh 160mg of PCL (molecular weight: 100,000), dissolve it in 2ml of a mixed solvent of dichloromethane and N,N-dimethylformamide (8:1, CH 2 Cl 2 / DMF, v / v), to prepare a PCL solution with a concentration of 80 mg / mL, seal it with a parafilm, stir it magnetically for 3 hours, and set aside.

[0036] (2) Put the PCL solution prepared in step (1) into the syringe of the electrospinning device, adjust the parameters of the micro propulsion pump, the capacity is 0.2 ml, the propulsion speed is 1.0 ml / hour, and run. Turn on the rotating device and adjust the speed to make the rotation stable. Turn on the high-voltage power supply, adjust the voltage value to 20 kV, start spinning, and receive the spinning along the direction of the tinfoil receiver.

[0037] (3) After spinning (about ten minutes), remove the tinfoil, and use a glass rod to remove the prepared spun fiber membrane along th...

Embodiment 2

[0044] 1. Preparation of electrospun fiber membranes of biodegradable materials:

[0045] (1) Weigh 200mg of PLGA (molecular weight 150,000, the ratio of lactic acid to glycolic acid is 9:1), dissolve it in 2ml of a mixed solvent of dichloromethane and N,N-dimethylformamide (6:1, CH 2 Cl 2 / DMF, v / v) to prepare a PLGA solution with a concentration of 100 mg / mL, seal it with parafilm, stir it magnetically for 3 hours, and set aside.

[0046] (2) Put the PLGA solution prepared in step (1) into the syringe of the electrospinning device, adjust the parameters of the micro propulsion pump, the capacity is 0.2 ml, the propulsion speed is 0.4 ml / hour, and run. Turn on the rotating device and adjust the speed to make the rotation stable. Turn on the high-voltage power supply, adjust the voltage value to 10 kV, start spinning, and receive the spinning along the direction of the tinfoil receiver.

[0047] (3) After spinning (about ten minutes), remove the tinfoil, and use a glass ro...

Embodiment 3

[0055] 1. Preparation of electrospun fiber membranes of biodegradable materials:

[0056] (1) Weigh 120mg of PLLA (molecular weight: 200,000), dissolve it in 2ml of a mixed solvent of dichloromethane and N,N-dimethylformamide (9:1, CH 2 Cl 2 / DMF, v / v) to prepare a PLLA solution with a concentration of 60 mg / mL, seal it with parafilm, stir it magnetically for 3 hours, and set aside.

[0057] (2) Put the PLLA solution prepared in step (1) into the syringe of the electrospinning device, adjust the parameters of the micro propulsion pump, the capacity is 0.2 ml, the propulsion speed is 0.5 ml / hour, and run. Turn on the rotating device and adjust the speed to make the rotation stable. Turn on the high-voltage power supply, adjust the voltage value to 14 kV, start spinning, and receive the spinning along the direction of the tinfoil receiver.

[0058] (3) After spinning (about ten minutes), remove the tinfoil, and use a glass rod to remove the prepared spun fiber membrane along ...

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Abstract

The invention discloses an electrostatic spinning nanofiber-extracellular matrix composite material as well as a preparation method and application thereof. The preparation method is simple and feasible in steps, and large-sized equipment is not needed. The prepared composite material has the advantages of electrostatic spinning nanofibers and an extracellular matrix. Compared with a pure electrostatic spinning nanofiber material or a pure extracellular matrix, the composite material has very high biocompatibility and very high mechanical property, is easier for cell adhesion and proliferation, has very good application prospect and high practical value in mechanical tissue engineering repair, and can be used for effectively promoting the adhesive growth, proliferation, migration and differentiation and amplifying and culturing stem cells on a large scale. Moreover, the composition material is low in antigenicity, and the risk of disease transmission is eliminated by means of strict screening.

Description

technical field [0001] The invention belongs to the technical field of polymer materials and tissue engineering, and specifically relates to an electrospinning nanofiber-extracellular matrix composite material and its preparation method and application. Background technique [0002] Cell carrier culture is a technology and method that can cultivate adherent cells on a large scale, and is currently the main method for large-scale culture of anchorage-dependent cells. Through the carrier medium that has no toxic side effects on the cells, the cells are attached to the surface or inside of the carrier, which can greatly increase the area and efficiency of cell culture, such as microcarrier culture technology, which has the advantages of homogeneous culture and both plate culture and suspension culture. The culture conditions (Temperature, pH value, carbon dioxide concentration, etc.) are easy to control, and the cultivation process is systematic and automatic, and it is not eas...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/44A61L27/50C12N5/0775D04H1/728
Inventor 龚逸鸿徐勇张栩杰何帆李燕蒋庆
Owner SUN YAT SEN UNIV
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