Calcium carbonate microsphere-extracellular matrix composite material as well as preparation method and application thereof

A composite material, calcium carbonate technology, applied in medical science, block delivery, prosthesis, etc., can solve the problems of unfavorable bone marrow mesenchymal stem cell adhesion and growth, poor biocompatibility, refractory degradation, etc. The effect of adhesion value-added, enhanced strength, and improved mechanical properties

Inactive Publication Date: 2015-05-27
SUN YAT SEN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the single calcium carbonate microsphere material has the disadvantages of poor biocompatibility, refractory and poor hydrophilicity, which is not conducive to the adhesion and growth of bone marrow mesenchymal stem cells.

Method used

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  • Calcium carbonate microsphere-extracellular matrix composite material as well as preparation method and application thereof
  • Calcium carbonate microsphere-extracellular matrix composite material as well as preparation method and application thereof
  • Calcium carbonate microsphere-extracellular matrix composite material as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1-3

[0033] (1) Preparation of calcium carbonate microspheres-extracellular matrix composites

[0034] The types and concentrations of the calcium salt solution and the carbonate solution of Examples 1-3 are shown in Table 1.

[0035] Table 1

[0036] calcium salt carbonate Example 1 Calcium chloride 25mM Sodium carbonate 25mM Example 2 Calcium nitrate 100 mM Sodium carbonate 100mM Example 3 Calcium chloride 250 mM Potassium carbonate 250mM

[0037] According to the type and concentration of Table 1, calcium salt solution and carbonate solution were mixed in equal volumes, and stirred at 1500 rpm / min for 10 minutes. Leave to stand at room temperature for 20 minutes, then freeze-dry for 24 hours. Put the obtained calcium carbonate microspheres into a 6-well plate containing medium (65 mg per well) and soak for 3 hours, and the seeding density per well is 30000 / cm 2 Bone marrow mesenchymal stem cells were placed in 95% CO at 37°C 2 Th...

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Abstract

The invention discloses a calcium carbonate microsphere-extracellular matrix composite material and its preparation method and application. The preparation method of the invention has simple steps and does not require large-scale equipment. The prepared composite material has the advantages of calcium carbonate microspheres and extracellular matrix. Compared with pure calcium carbonate microspheres or extracellular matrix materials, it has good biocompatibility and good mechanical mechanics It has high performance and is easier for cell adhesion and value-added. It has high application prospects and practical value in medical tissue engineering repair, and can effectively promote cell adhesion growth, proliferation, migration and differentiation.

Description

technical field [0001] The invention belongs to the field of tissue engineering, and in particular relates to a calcium carbonate microsphere-extracellular matrix composite material and its preparation method and application. Background technique [0002] Bioscaffold material is a very important part of tissue engineering. It is not only a carrier for cells and biologically active factors to enter the body, but also can support the proliferation and differentiation of cells in vivo and secrete active cytokines. An ideal scaffold for adipose tissue engineering should have: ① a three-dimensional structure suitable for cell adhesion, proliferation and differentiation; ② good mechanical properties, not only matching the characteristics of the host soft tissue, but also supporting the seed cells against the pressure of the soft tissue ③has good composition, non-toxic to tissue, no side effects, no immunogenicity; ④has special biological stimulation, such as promoting cell prolif...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K9/16A61L27/22A61L27/44
Inventor 龚逸鸿许哲武徐勇李燕张志光蒋庆
Owner SUN YAT SEN UNIV
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