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A kind of electrospinning nanofiber-extracellular matrix composite material and its preparation method and application

A technology of electrospinning and nanofibers, which is applied in the field of electrospinning nanofibers-extracellular matrix composite materials and its preparation, can solve the problems of ineffective resistance to soft tissue pressure and poor mechanical properties, and achieve high application prospects and Practical value, good mechanical properties, and the effect of improving mechanical properties

Inactive Publication Date: 2016-04-20
SUN YAT SEN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, purely using extracellular matrix as a tissue engineering repair material has disadvantages such as poor mechanical properties and inability to effectively resist the pressure of soft tissues.

Method used

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  • A kind of electrospinning nanofiber-extracellular matrix composite material and its preparation method and application
  • A kind of electrospinning nanofiber-extracellular matrix composite material and its preparation method and application
  • A kind of electrospinning nanofiber-extracellular matrix composite material and its preparation method and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] 1. Preparation of electrospun fiber membranes of biodegradable materials:

[0035] (1) Weigh 160mg of PCL (molecular weight: 100,000), dissolve it in 2ml of a mixed solvent of dichloromethane and N,N-dimethylformamide (8:1, CH 2 Cl 2 / DMF, v / v) to prepare a PCL solution with a concentration of 80mg / mL, seal it with a parafilm, stir it magnetically for 3 hours, and set aside.

[0036](2) Put the PCL solution prepared in step (1) into the syringe of the electrospinning device, adjust the parameters of the micro propulsion pump, the capacity is 0.2 ml, the propulsion speed is 1.0 ml / hour, and run. Turn on the rotating device and adjust the speed to make the rotation stable. Turn on the high-voltage power supply, adjust the voltage value to 20 kV, start spinning, and receive the spinning along the direction of the tinfoil receiver.

[0037] (3) After spinning (about ten minutes), remove the tinfoil, and use a glass rod to remove the prepared spun fiber membrane along the...

Embodiment 2

[0043] 1. Preparation of electrospun fiber membranes of biodegradable materials:

[0044] (1) Weigh 200mg of PLGA (molecular weight 150,000, the ratio of lactic acid to glycolic acid is 9:1), dissolve it in 2ml of a mixed solvent of dichloromethane and N,N-dimethylformamide (6:1, CH 2 Cl 2 / DMF, v / v) to prepare a PLGA solution with a concentration of 100 mg / mL, seal it with parafilm, stir it magnetically for 3 hours, and set aside.

[0045] (2) Put the PLGA solution prepared in step (1) into the syringe of the electrospinning device, adjust the parameters of the micro propulsion pump, the capacity is 0.2 ml, the propulsion speed is 0.4 ml / hour, and run. Turn on the rotating device and adjust the speed to make the rotation stable. Turn on the high-voltage power supply, adjust the voltage value to 10 kV, start spinning, and receive the spinning along the direction of the tinfoil receiver.

[0046] (3) After spinning (about ten minutes), remove the tinfoil, and use a glass ro...

Embodiment 3

[0053] 1. Preparation of electrospun fiber membranes of biodegradable materials:

[0054] (1) Weigh 120mg of PLLA (molecular weight: 200,000), dissolve it in 2ml of a mixed solvent of dichloromethane and N,N-dimethylformamide (9:1, CH 2 Cl 2 / DMF, v / v) to prepare a PLLA solution with a concentration of 60 mg / mL, seal it with a parafilm, stir it magnetically for 3 hours, and set aside.

[0055] (2) Put the PLLA solution prepared in step (1) into the syringe of the electrospinning device, adjust the parameters of the micro propulsion pump, the capacity is 0.2 ml, the propulsion speed is 0.5 ml / hour, and run. Turn on the rotating device and adjust the speed to make the rotation stable. Turn on the high-voltage power supply, adjust the voltage value to 14 kV, start spinning, and receive the spinning along the direction of the tinfoil receiver.

[0056] (3) After spinning (about ten minutes), remove the tinfoil, and use a glass rod to remove the prepared spun fiber membrane alon...

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Abstract

The invention discloses an electrospinning nanofiber-extracellular matrix composite material and its preparation method and application. The preparation method of the invention has simple steps and does not require large-scale equipment. The prepared composite material has both the advantages of electrospun nanofibers and extracellular matrix. Compared with pure electrospun nanofibers or extracellular matrix materials, it has good biocompatibility and good biocompatibility. Mechanical properties, easier for cell adhesion and value-added, have high application prospects and practical value in medical tissue engineering repair, can effectively promote cell adhesion growth, proliferation, migration and differentiation, and can expand and culture stem cells on a large scale. In addition, the composite material also has low antigenicity and has been strictly screened, so there is no risk of disease transmission.

Description

technical field [0001] The invention belongs to the technical field of polymer materials and tissue engineering, and specifically relates to an electrospinning nanofiber-extracellular matrix composite material and its preparation method and application. Background technique [0002] Cell carrier culture is a technology and method that can cultivate adherent cells on a large scale, and is currently the main method for large-scale culture of anchorage-dependent cells. Through the carrier medium that has no toxic side effects on the cells, the cells are attached to the surface or inside of the carrier, which can greatly increase the area and efficiency of cell culture, such as microcarrier culture technology, which has the advantages of homogeneous culture and both plate culture and suspension culture. The culture conditions (Temperature, pH value, carbon dioxide concentration, etc.) are easy to control, and the cultivation process is systematic and automatic, and it is not eas...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N5/071A61L27/44A61L27/50C12N5/0775D04H1/728
Inventor 龚逸鸿徐勇张栩杰何帆李燕蒋庆
Owner SUN YAT SEN UNIV
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