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Oriented polymer nanometer fibrocyte culture plate and preparation method thereof

A nanofiber and cell culture technology, which is applied in biochemical equipment and methods, tissue cell/virus culture devices, and methods of supporting/fixing microorganisms, etc. Industrial application and other issues, to achieve the effect of no cytotoxicity, maintaining cell viability, and facilitating cell adhesion and growth

Active Publication Date: 2015-07-15
广州永翔科技发展有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, other collection devices have problems such as complex devices, low yield of nanofibers, or small area of ​​fiber orientation arrangement, which limits industrial application.

Method used

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  • Oriented polymer nanometer fibrocyte culture plate and preparation method thereof
  • Oriented polymer nanometer fibrocyte culture plate and preparation method thereof
  • Oriented polymer nanometer fibrocyte culture plate and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0068] Preparation of oriented polymer nanofiber 6-well cell culture plates:

[0069] A standard 6-well flat-bottomed cell culture plate made of ordinary polystyrene, which is sterilized as the fixed object of the oriented polymer nanofiber scaffold. First, mix the polymer raw materials PLGA, PCL and collagen at a mass ratio of 7: 2.5: 0.5, add a small amount of NGF (nerve growth factor) as the bioactive component to the polymer raw materials, the mixing ratio is 100:1, add 1:1 The mass fraction of the solute in the dichloromethane / chloroform mixed solvent was about 10 wt%, and it was fully dissolved to prepare the electrospinning solution. Then, the electrospinning solution was sucked into the electrospinning syringe, and the electrospinning was carried out under the condition that the needle tip voltage of the syringe was 30 kV and the pump supply volume was 0.030 ml / min-0.050 ml / min, and the obtained fiber diameter was Between 50nm~300nm. The obtained oriented PLGA / PCL / co...

Embodiment 2

[0076] Preparation of oriented polymer nanofiber 24-well cell culture plates:

[0077]A 24-well standard flat-bottomed cell culture plate made of ordinary polystyrene, which is sterilized and fixed as an orientation polymer nanofiber scaffold. First, the polymer raw material silk fibroin was dissolved in a supersaturated LiBr aqueous solution with a silk fibroin concentration of 10 wt%, and then after successive steps of dilution, centrifugation, filtration, dialysis and concentration, the silk fibroin concentration was about 30 wt%. % concentrated aqueous solution. Add a small amount of calcium chloride (concentration is about 0.3 mol / L) to the concentrated silk fibroin solution, and then add a small amount of BMPs (bone morphogenetic protein) and nano-hydroxyapatite to the polymer as bioactive components Raw materials, the mixing ratios of the two and silk fibroin are 1:50 and 1:200 respectively, fully dissolved and dispersed to obtain an electrospinning solution for use. ...

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Abstract

The invention discloses a cell culture plate based on oriented polymer nanometer fiber and a preparation method thereof. The cell culture plate comprises a standard flat-bottom cell culture plate used as a main body and a polymer nanometer fiber support adhered on the hole bottom of the culture plate. The polymer nanometer fiber support is prepared by adding bioactive components into the main raw material--polymer, carrying out grafting or blending, then carrying out electrostatic spinning and carrying out collection by using a fiber collecting device of a parallel conductive panel; and fiber bundles on the polymer nanometer fiber support are of a regular oriented arrangement structure. The culture plate provided by the invention has good biocompatibility and is applicable to culture of cells or tissue with oriented morphological characteristics, especially to culture and regeneration of cells and related tissue of nerves, bones, muscles, ligaments, etc.

Description

technical field [0001] The invention belongs to the field of cell and tissue culture consumables, and in particular relates to a cell culture plate based on an oriented polymer nanofiber scaffold and a preparation method thereof, which can be used for culturing and regeneration of cells or tissues with oriented morphological characteristics. Background technique [0002] Tissue regeneration has been at the forefront of the biomedical field in recent years, and the cell culture scaffold in vitro is the basis of tissue regeneration. As an artificial extracellular matrix (ECM), it provides a three-dimensional space for cell docking, growth, reproduction, and metabolism to support cell growth. for complete organization. Therefore, scaffold materials for cell culture in vitro have become the focus of tissue engineering research. In addition to good biocompatibility and biodegradability, scaffold materials for in vitro cell culture also need to have certain hydrophilicity and goo...

Claims

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Application Information

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IPC IPC(8): C12M3/00D01D5/00
CPCC12M25/06D01D5/003C12M25/02
Inventor 赵鹏文学军李丘沐刘静
Owner 广州永翔科技发展有限公司
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