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Method for preparing polypeptide copolymer porous nanofiber by using electrostatic spinning

A technology of electrospinning and nanofibers, which is applied in the direction of spinning solution preparation, single-component copolyamide artificial filament, fiber treatment, etc., can solve problems such as constraints and poor cell adhesion performance, and achieve good biophase Capacitance, effect of promoting cell adhesion and growth

Inactive Publication Date: 2014-02-19
EAST CHINA UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, since most synthetic polypeptides are hydrophobic substances, their cell adhesion performance is not good, so their application in the field of tissue engineering is restricted.

Method used

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  • Method for preparing polypeptide copolymer porous nanofiber by using electrostatic spinning
  • Method for preparing polypeptide copolymer porous nanofiber by using electrostatic spinning
  • Method for preparing polypeptide copolymer porous nanofiber by using electrostatic spinning

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] A method for preparing polypeptide copolymer porous nanofibers by electrospinning, the preparation steps are:

[0032] (1) Hydrophilic modification of homopolypeptide

[0033] For hydrophobic peptides—poly( -Benzyl L-glutamate) (PBLG) for hydrophilic modification:

[0034] In a 250mL reaction bottle, add 2g of poly( -benzyl L-glutamate) (PBLG), 100mL refined dioxane and 3mL ethanolamine (ETA), reacted with electromagnetic stirring at 60°C for 11 hours, then precipitated, filtered, and dried in vacuo to obtain white poly( -Benzyl L-glutamate-hydroxyethylglutamine) [P(BLG-HEG)] solid 1.8 g; degree of substitution with ethanolamine (ETA) 5%.

[0035] (2) Preparation of spinning dope

[0036] 0.16g of poly( -Benzyl L-glutamate-hydroxyethylglutamine)[P(BLG-HEG)] random copolymer was dissolved in 2 mL of volatile organic solvent tetrahydrofuran / dichloromethane with volume ratio of 80:20 (v:v) In a compound solvent of methane (THF / DCM), a solution with a concentratio...

Embodiment 2

[0045] A method for preparing polypeptide copolymer porous nanofibers by electrospinning, the preparation steps are:

[0046] (1) Hydrophilic modification of homopolypeptide

[0047] Basic content is the same as embodiment 1, and difference is:

[0048] Poly( The molecular weight of -benzyl L-glutamate) (PBLG) is 230000g / mol, ethanolamine (ETA) is 6mL, the reaction time of electromagnetic stirring under 60 ℃ is 20 hours, and the substitution degree of ethanolamine (ETA) is 11%.

[0049] (2) Preparation of spinning dope

[0050] 0.14g of poly( -Benzyl L-glutamate-hydroxyethylglutamine) [P(BLG-HEG)] random copolymer is dissolved in the volatile organic solvent trichloromethane / tri In the compound solvent of fluoroacetic acid (TCM / TFA), stir evenly, and prepare the electrospinning stock solution with a concentration of 5% (w / v).

[0051] (3) Perform high-voltage electrospinning

[0052] The electrospinning stock solution in step (2) was subjected to high-voltage electrosp...

Embodiment 3

[0058] A method for preparing polypeptide copolymer porous nanofibers by electrospinning, the preparation steps are:

[0059] (1) Hydrophilic modification of the homopolypeptide (same as Example 2).

[0060] (2) Preparation of spinning dope

[0061] 0.26g of poly( -Benzyl L-glutamate-hydroxyethylglutamine) [P(BLG-HEG)] random copolymer is dissolved in the volatile organic solvent trichloromethane / tri In a compound solvent of fluoroacetic acid (TCM / TFA), stir evenly to prepare an electrospinning stock solution with a concentration of 13% (w / v).

[0062] (3) Perform high-voltage electrospinning

[0063] The electrospinning stock solution in step (2) was subjected to high-voltage electrospinning under the conditions of a spinning voltage of 13kV, a receiving distance from the spinneret to the aluminum foil of 13cm, and an extrusion flow rate of 1mL / h.

[0064] (4) vacuum drying

[0065] (Same as Example 1), the target product—porous nanofibers with high specific surface are...

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Abstract

The invention relates to a method for preparing polypeptide copolymer porous nanofiber by using electrostatic spinning. The method comprises the following steps: firstly, carrying out hydrophilic modification on a hydrophobic polypeptide homopolymer to prepare a poly(gamma-benzyl L-glutamate-hydroxyethyl glutamine) or poly(gamma-benzyl L-glutamine)-g-polyethylene glycol grafted copolymer; secondly, preparing a compound solvent by using solvents with different volatilization properties, and then preparing into a spinning solution; thirdly, spinning by using a high voltage electrostatic spinning method; and finally, placing spun yarns into a vacuum drying chamber and drying for more than 3 hours at room temperature to obtain the porous nanofiber with high specific surface area. A cytotoxicity test indicates that the prepared porous nanofiber with high specific surface area is free of cytotoxicity and good in biocompatibility, can be used as a porous scaffold for cell growth, has the function of promoting cell migration and amplification and has a relatively good application value in the aspect of preparation of scaffolds for tissue engineering; besides, the polypeptide copolymer porous nanofiber has an application prospect in the fields of medical dressing, medicine released control, artificial organs, sewage treatment and the like.

Description

technical field [0001] The invention relates to the technical field of preparation of functional fibers, in particular to a method for preparing porous nanofibers of polypeptide copolymers by electrospinning. Background technique [0002] After the 1990s, with the continuous development of nanotechnology, electrospinning has become one of the main methods for preparing nanofibers. Electrospinning is a method in which a polymer solution or melt is sprayed and stretched under electrostatic action to obtain nano or micron fibers. The materials that can be used for electrospinning are very wide, including most synthetic polymers and some natural polymer materials, such as chitosan and gelatin. Synthetic polypeptides are a class of excellent biodegradable materials. They have no toxic side effects on organisms, have the same main chain structure as natural proteins, and have good biocompatibility. They are widely used in drug sustained release and medical adhesives. and other b...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): D01F6/80D01D5/00D01D1/02C08G69/48
Inventor 陈涛张帆林嘉平林绍粱
Owner EAST CHINA UNIV OF SCI & TECH
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