Full liquid phase organ chip and preparation method thereof

Abstract

The invention discloses a full liquid phase organ chip and a preparation method thereof. The full liquid phase organ chip comprises a super hydrophobic porous membrane with a hydrophilic channel, theporous membrane is sealed in an oil phase, the hydrophilic channel is provided with a fluid inlet and a fluid outlet, and the fluid inlet and the fluid outlet communicate with the hydrophilic channel;the polymer layer porous membrane is placed in a functional modification solution to obtain a hydrophobic polymer layer porous membrane; hydrophilic patterns are treated on the hydrophobic polymer layer porous membrane; then, polymer surface superhydrophobic treatment is carried out on the surface of the porous membrane with the hydrophilic channel; and then the hydrophilic channel on the surfaceof the porous membrane is wetted and sealed in the oil phase, and an inlet and an outlet which communicate with the hydrophilic channel are formed. According to the full liquid phase organ chip and the preparation method thereof, the preparation conditions are mild, the whole liquid phase organ chip can be constructed by constructing cell patterned culture on a prepared substrate, and the in-situdetection and sampling of all micro environments in the organ chip can be realized, and the spatial editing of the function and geometric layout of a fluid device can be realized as required.

Description

technical field [0001] The invention relates to the field of biochips and preparation methods thereof, in particular to an all-liquid phase organ chip and a preparation method thereof. Background technique [0002] New drug research and development is a time-consuming and huge investment industry. At present, the average research and development cost of a new drug is about 1 billion US dollars, and it takes an average of 10 years, making the development of new drugs extremely difficult. At present, 90% of drugs that have passed cell experiments and animal experiments in drug screening will be rejected in the clinical testing stage. The main reason is that there are large differences between cell experiments and animal models and human models, so the development is closer to the human body Model drug testing platform is extremely important. In recent years, with the rapid development of biotechnology and information technology, organ-on-a-chip has become a distinctive and d...

AI Technical Summary

Problems solved by technology

In addition, some tests, such as cell sequencing, need to take samples from the inside of the chip, so the organ chip needs to integrate sampling flow channels and interfaces, which makes the whole system very complicated, which greatly restricts the development of the organ chip

In addition, the current organ chip is difficult to change its structure after construction, which makes it unable to simulate the dynamic changes of human tissue, and in some cases cannot achieve bionic functions well.

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