3results about How to "Solve detection difficulties" patented technology

The invention relates to the technical field of computer vision, and discloses an object detection and reconstruction method and device, electronic equipment and a storage medium, and the method comprises the steps: obtaining an image sequence of an image source, carrying out the preprocessing, and dividing a preprocessed image into image combinations; training an initial object detection model based on the sample image combination, and adding a motion state prediction branch to the initial object detection model; detecting the image combination by using a target object detection model to obtain a detection result; and screening out a target detection result consistent with the object category and the motion state of the target object from the detection results of all image combinations representing the same target time, and performing reconstruction based on the target detection result to obtain a reconstruction coordinate. By adding a motion state prediction branch, the problem of difficult detection caused by smear of a high-speed moving object can be solved, the motion / static state can be accurately distinguished, and the detection accuracy and comprehensiveness are improved. And detection results are screened through time, categories and motion states, so that the reconstruction precision and efficiency are improved.

The application discloses a kind of for electron irradiation accelerator beam under gas environment monitoring and processing method, it is related to electron irradiation accelerator technical field, including the following steps: ozone generated by electron beam irradiation is through shielding body upper side ventilation system first gas collection port, is transported to shielding body outer exhaust duct by exhaust duct in shielding body, most of probe is excluded from beam under high-dose irradiation environment, ensure stable operation, while ozone concentration and smoke state of the probe can be detected in beam under irradiation space.The application combines the design structure of shielding body and gas drainage method, realizes the real-time monitoring of electron beam irradiation space environment gas.According to the structural characteristics of shielding body, the ventilation and beam under environment detection system are integrated, the beam under gas environment detection problem is solved, the service life is prolonged, the irradiation accelerator operation stability is improved, the humidity is monitored by humidity sensor and the air supply amount is adjusted, the real-time monitoring is realized, and the pollution of harmful gas to irradiation space environment is reduced.

The application discloses a method for semi-quantitative analysis of expression level of secreted protein of human joint cartilage cells by using immunoblotting technology. The method comprises the following steps: 1) preparing a secreted protein culture solution; 2) collecting a sample of the secreted protein culture solution; 3) performing dialysis desalting treatment on the sample; 4) concentrating the secreted protein culture solution to prepare a powder; 5) re-dissolving the secreted protein culture solution powder and quantifying the protein; 6) separating the protein components in the re-dissolved culture solution; and 7) detecting the expression level of the secreted protein in the re-dissolved culture solution sample by using immunoblotting method. The low-temperature dialysis and vacuum concentration technology can significantly improve the concentration of the secreted protein in the human joint cartilage tissue culture solution sample under the condition of maintaining the integrity of the protein structure, and is suitable for detecting the chemical fluorescence signal of the secreted protein in the sample by using the immunoblotting technology. The application provides an important cell reaction detection tool for studying the pathogenesis of bone and joint diseases caused by degenerative changes of cartilage tissue and researching and developing biological small-molecule drugs for treating the diseases.